Biosynthesis and mobilization of a novel polyhydroxyalkanoate containing 3-hydroxy-4-methylvalerate monomer produced by Burkholderia sp. USM (JCM15050)

Lau, Nyok-Sean; Chee, Jiun-Yee; Tsuge, Takeharu; Sudesh, Kumar

doi:10.1016/j.biortech.2010.05.049

Cited by 23 publications

(20 citation statements)

References 26 publications

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“…droxyvalerate-co-4-hydroxybutyrate) P(3HB-co-3HV --co-4HB), have been produced by batch fermentation. [40][41][42][43][44][45][46][47][48][49][50] Production of mcl-PHAs and other interesting PHA copolymers including scl-copolymers and scl-mcl copolymers has also been reported using unconventional substrates, such as lignocellulosics e.g., grass mass hydrolysate, rice bran, rapeseed meal hydrolysate, and industrial waste e.g., whey, biodiesel waste, vinnase etc. 35,48,[51][52][53][54][55][56] Utilization of waste whey for PHA production is particularly useful as only 50 % of the whey produced is used for production of food ingredients and animal feed, while the rest remains as a pollutant owing to its high biological oxygen demand.…”

Section: Ta B L E 1 -Summary Of Results Of Batch Fermentation For Promentioning

confidence: 99%

Strategies for Large-scale Production of Polyhydroxyalkanoates

Kaur

Roy

2015

Chem.Biochem.Eng.Q.

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Polyhydroxyalkanoates (PHAs) are biodegradable and biocompatible intracellular polyesters that are accumulated as energy and carbon reserves by bacterial species, under nutrient limiting conditions. Successful large-scale production of PHAs is dependent on three crucial factors, which include the cost of substrate, downstream processing cost, and process development. In this respect, design and implementation of bioprocess strategies for efficient PHA bioconversions enable high PHA concentrations, yields and productivities. Additionally, development of PHA fermentation processes using inexpensive substrates, such as agro-industrial wastes, facilitates further cost reduction, thus benefitting large-scale PHA production. Thus, the aim of this review is to highlight various bioprocess strategies for high production of PHAs and their novel copolymers in relatively large quantities. This review also discusses the application of kinetic analysis and mathematical modelling as important tools for process optimization and thus improvement of the overall process economics for large-scale production of PHAs.

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Section: Ta B L E 1 -Summary Of Results Of Batch Fermentation For Promentioning

confidence: 99%

Strategies for Large-scale Production of Polyhydroxyalkanoates

Kaur

Roy

2015

Chem.Biochem.Eng.Q.

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“…Inoculum was prepared by cultivating the bacterium in 50 mL of nutrient rich (NR) broth at 30 C, 200 rpm and an initial pH 7.0. The components of NR broth (per liter) were 10.0 g peptone, 10.0 g meat extract and 2.0 g yeast extract. After 5 h, 3% (v/v) of inoculum (OD 600nm ¼ 4.5) was transferred into 200 mL of nitrogen-limiting mineral salts medium (MM) in 1 L conical flask and was cultivated at 30 C, 200 rpm for 48 h and an initial (pH 7.0).…”

Section: Pha Synthesis and Purificationmentioning

confidence: 99%

“…Isocaproic acid is a new structurally related carbon source or a precursor in PHA production. Recent studies reported that novel 3-hydroxy-4-methylvalerate (3H4MV) monomer can be synthesized by feeding isocaproic acid to various strains of bacteria [8,10]. The material properties and stability of the copolymer containing 3H4MV monomer shows clear improvement.…”

Section: Introductionmentioning

confidence: 98%

Biosynthesis and characterization of novel polyhydroxyalkanoate polymers with high elastic property by Cupriavidus necator PHB−4 transformant

Chia

Ooi

Saika

et al. 2010

Polymer Degradation and Stability

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“…Novel PHAs with different monomer constituents have been identifi ed by natural PHA-producing bacteria such as the production of poly(3-hydroxybutyrate-co-3-mercaptopropionate) [P(3HB-co-3MP)] from Cupriavidus necator (Lütke-Eversloh et al, 2002), as well as the production of poly(3-hydroxybutyrate-co-3-hydroxy-4-methylvalerate) [P(3HB-co-3H4MV)] from Burkholderia sp. (Lau et al, 2010) and Chromobacterium sp. USM2 (Ling et al, 2011).…”

Section: Introductionmentioning

confidence: 99%

Screening and identification of polyhydroxyalkanoates producing bacteria and biochemical characterization of their possible application

Sangkharak

Prasertsan

2012

J. Gen. Appl. Microbiol.

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Polyhydroxyalkanoates (PHAs) accumulating bacteria were isolated under various selective conditions such as pH, salt concentrations and types of heavy metal. Fifty strains of bacterial isolates were found to belong to Bacillus, Proteus, Pseudomonas, Aeromonas, Alcaligenes and Chromobacterium, based on phenotypical features and genotypic investigation. Only twenty fi ve bacterial isolates were selected and observed for the production of PHAs. Interestingly, bacteria belonging to Firmucutes Bacillus sp. produced a high amount of PHAs. The maximum PHAs were accumulated by B. licheniformis PHA 007 at 68.80% of dry cell weight (DCW). Pseudomonas sp., Aeromonas sp., Alcaligenes sp. and Chromobacterium sp. were recorded to produce a moderate amount of PHAs, varying from 10.00 44.32% of DCW. The enzymatic activity was preliminarily analyzed by the ratio of the clear zone diameter to colony diameter. Bacillus gave the highest ratio of hydrolysis zone which corresponds to the highest hydrolytic enzyme activities. Bacillus licheniformis PHA 007 had the highest lipase and protease activity at 2.1 and 5.1, respectively. However, the highest amylase activity was observed in Bacillus sp. PHA 023 at 1.4. Determination of metabolic characteristics was also investigated to check for their ability to consume a wide range of substrates. Bacillus, Aeromonas sp. and Alcaligenes sp. had great ability to utilize a variety of substrates. To decrease high PHA cost, different sources of cheap substrates were tested for the production of PHAs. Bacillus cereus PHA 008 gave the maximal yield of PHA production (64.09% of DCW) when cultivated in anaerobically treated POME. In addition, the accumulation of PHA copolymers such as 3-hydroxyvalerate and 3-hydroxyhexanoate was also observed in Bacillus and Pseudomomas sp. strain 012 and 045, respectively. Eight of the nine isolates accumulated a signifi cant amount of PHAs when inexpensive carbon sources were used as substrates. Here it varied from 1.69% of DCW by B. licheniformis PHA 007 to 64.09% of DCW by B. cereus PHA 008.

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Biosynthesis and mobilization of a novel polyhydroxyalkanoate containing 3-hydroxy-4-methylvalerate monomer produced by Burkholderia sp. USM (JCM15050)

Cited by 23 publications

References 26 publications

Strategies for Large-scale Production of Polyhydroxyalkanoates

Strategies for Large-scale Production of Polyhydroxyalkanoates

Biosynthesis and characterization of novel polyhydroxyalkanoate polymers with high elastic property by Cupriavidus necator PHB−4 transformant

Screening and identification of polyhydroxyalkanoates producing bacteria and biochemical characterization of their possible application

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