Biosynthesis and secretion of tropoelastin by chick aorta cells

Rosenbloom, Joel; Cywinski, Anita

doi:10.1016/0006-291x(76)90920-7

Cited by 51 publications

(18 citation statements)

References 13 publications

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“…1). The identity of this peak as soluble elastin has been previously confirmed by us (4,9) and others (5)(6)(7)(8). The total radioactivity under the peak can be summed, corrected for the size of aliquot taken for SDS gel electrophoresis, and the synthesis of soluble elastin expressed as dpm per 100 mg (wet weight) of tissue per hour of incubation.…”

Section: Hntrodncgansupporting

confidence: 73%

The synthesis of soluble and Insoluble elastin in chicken aorta as a function of development and age. Effect of a high cholesterol diet

Fw¹

1979

Can. J. Biochem.

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The synthesis of soluble elastin, newly synthesized insoluble elastin and total accumulated insoluble elastin was measured in aortic tissue of chickens ranging in age from the 11-day embryo to the adult chicken. Synthesis of soluble elastin reached a maximum in the 1st week after hatching, then decreased rapidly with a second transient increase between 4 and 6 weeks and thereafter decreased continuously until synthesis could no longer be detected in the 35-week-old adult. A portion of this newly synthesized soluble elastin was insolubilized even during 1 h of incubation by a β-aminopropionitrile-inhibited mechanism. Total insoluble elastin accumulated rapidly in aortic tissue in the late embryonic stages and reached a plateau about 1 week after hatching, after which time the proportion of the protein in the tissue remained constant. Synthesis of soluble elastin and total insoluble elastin was also determined in aortic tissue of chickens raised on a diet containing 2% cholesterol for 14 weeks after hatching. This cholesterol-rich diet had an early, but transient effect on the synthesis of soluble elastin, shifting the age of maximal synthesis to 1 to 2 weeks after hatching. However, by 6 to 8 weeks on the diet there was no difference in soluble elastin synthesis between normal and cholesterol-fed groups. Although prolonged cholesterol feeding resulted in serum cholesterol levels 10 times normal, aortic tissue cholesterol levels 3 times normal and grossly visible atherosclerotic lesions, no reinitiation of soluble elastin synthesis or alteration in the quantity and character of insoluble aortic elastin could be detected.

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Section: Hntrodncgansupporting

confidence: 73%

The synthesis of soluble and Insoluble elastin in chicken aorta as a function of development and age. Effect of a high cholesterol diet

Fw¹

1979

Can. J. Biochem.

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“…The asymmetric form of acetylcholinesterase is composed of catalytic subunits attached to a collagenous, triple-helical domain (Rosenbloom and Cywinski, 1976b). Acetylcholinesterase hydrolyzes the neurotransmitter acetylcholine, which ends signal transmission at neuromuscular junctions.…”

Section: Prolyl 4-hydroxylasementioning

confidence: 99%

“…A prototypical elastin sequence is Val–Pro–Gly–Val–Gly, and peptides composed of (Val–Pro–Gly–Val–Gly) n repeats are substrates for P4H (Bhatnagar et al , 1978). The creation of Hyp in elastin is catalyzed by the collagen P4H, but there is less Hyp in elastin than in collagen (Rosenbloom and Cywinski, 1976b) and Hyp is not required for elastin biosynthesis or secretion (Rosenbloom and Cywinski, 1976a). The accumulation of elastin is, however, affected by levels of ascorbic acid.…”

Section: Prolyl 4-hydroxylasementioning

confidence: 99%

Prolyl 4-hydroxylase

Gorres

Raines

2010

Critical Reviews in Biochemistry and Molecular Biology

542

591

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Posttranslational modifications can cause profound changes in protein function. Typically, these modifications are reversible, and thus provide a biochemical on–off switch. In contrast, proline residues are the substrates for an irreversible reaction that is the most common posttranslational modification in humans. This reaction, which is catalyzed by prolyl 4-hydroxylase (P4H), yields (2S,4R)-4-hydroxyproline (Hyp). The protein substrates for P4Hs are diverse. Likewise, the biological consequences of prolyl hydroxylation vary widely, and include altering protein conformation and protein–protein interactions, and enabling further modification. The best known role for Hyp is in stabilizing the collagen triple helix. Hyp is also found in proteins with collagen-like domains, as well as elastin, conotoxins, and argonaute 2. A prolyl hydroxylase domain protein acts on the hypoxia inducible factor α, which plays a key role in sensing molecular oxygen, and could act on inhibitory κB kinase and RNA polymerase II. P4Hs are not unique to animals, being found in plants and microbes as well. Here, we review the enzymic catalysts of prolyl hydroxylation, along with the chemical and biochemical consequences of this subtle but abundant posttranslational modification.

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“…Many of these are involved in innate and adaptive immune responses, as well as in the recognition/presentation of multivalent immune epitopes (46). Elastin (51) and argonaute2, which is involved in RISC complexes (52), are also substrates of P4H.…”

Section: Chemistry Of Proline Hydroxylasesmentioning

confidence: 99%

Oxygen, Metabolism, and Regeneration: Lessons from Mice

Heber‐Katz

2017

Trends in Molecular Medicine

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The discovery that the Murphy Roths Large (MRL) mouse strain is a fully competent, epimorphic tissue regenerator, proved that the machinery of regeneration was preserved through evolution from hydra, to salamanders, to mammals. Such concepts have allowed translation of the biology of amphibians -- and their ability to regenerate -- to a mammalian context. In particular, the ancient hypoxia inducible factor (HIF-1α) pathway, operating through prolyl hydroxylase domain proteins (PHDs), was identified as a central player in mouse regeneration. Thus, the possibility of targeting PHDs or other HIF-1α modifiers to effectively recreate the amphibian regenerative state has emerged. We posit that these regenerative pathways are critical in mammals. Moreover, the current approved use of PHD inhibitors in the clinic should allow fast-track translation from mouse studies to drug-based regenerative therapy in humans.

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Biosynthesis and secretion of tropoelastin by chick aorta cells

Cited by 51 publications

References 13 publications

The synthesis of soluble and Insoluble elastin in chicken aorta as a function of development and age. Effect of a high cholesterol diet

The synthesis of soluble and Insoluble elastin in chicken aorta as a function of development and age. Effect of a high cholesterol diet

Prolyl 4-hydroxylase

Oxygen, Metabolism, and Regeneration: Lessons from Mice

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