Biosynthesis, biotechnological production, and application of teicoplanin: current state and perspectives

Jung, Hae Woon; Jeya, Marimuthu; Kim, Sang-Yong; Moon, Hee-Jung; Singh, Raushan Kumar; Zhang, Ye-Wang; Lee, Jung-Kul

doi:10.1007/s00253-009-2107-4

Cited by 36 publications

(29 citation statements)

References 101 publications

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“…Teicoplanin is produced by fermenting A. teichomyceticus ATCC 31121, as a complex of structurally related molecules differing in the length and branching of the fatty acid moiety linked to the glucosamine residue on the heptapeptide scaffold [4]. The common core of teicoplanin (Figure 1A) consists of seven aromatic amino acids, which, apart from the tyrosine residue at position 2, are nonproteinogenic amino acids: three p -hydroxyphenylglycine residues at positions 1, 4, and 5; two dihdroxyphenylglycine at positions 3 and 7; and one β-hydroxytyrosine at position 6.…”

Section: Introductionmentioning

confidence: 99%

Actinoplanes teichomyceticus ATCC 31121 as a cell factory for producing teicoplanin

Taurino¹,

Frattini

Marcone

et al. 2011

Microb Cell Fact

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BackgroundTeicoplanin is a glycopeptide antibiotic used clinically in Europe and in Japan for the treatment of multi-resistant Gram-positive infections. It is produced by fermenting Actinoplanes teichomyceticus. The pharmaceutically active principle is teicoplanin A2, a complex of compounds designated T-A2-1-A2-5 differing in the length and branching of the fatty acid moiety linked to the glucosamine residue on the heptapeptide scaffold. According to European and Japanese Pharmacopoeia, components of the drug must be reproduced in fixed amounts to be authorized for clinical use.ResultsWe report our studies on optimizing the fermentation process to produce teicoplanin A2 in A. teichomyceticus ATCC 31121. Robustness of the process was assessed on scales from a miniaturized deep-well microtiter system to flasks and 3-L bioreactor fermenters. The production of individual factors T-A2-1-A2-5 was modulated by adding suitable precursors to the cultivation medium. Specific production of T-A2-1, characterized by a linear C10:1 acyl moiety, is enhanced by adding methyl linoleate, trilinoleate, and crude oils such as corn and cottonseed oils. Accumulation of T-A2-3, characterized by a linear C10:0 acyl chain, is stimulated by adding methyl oleate, trioleate, and oils such as olive and lard oils. Percentages of T-A2-2, T-A2-4, and, T-A2-5 bearing the iso-C10:0, anteiso-C11:0, and iso-C11:0 acyl moieties, respectively, are significantly increased by adding precursor amino acids L-valine, L-isoleucine, and L-leucine. Along with the stimulatory effect on specific complex components, fatty acid esters, oils, and amino acids (with the exception of L-valine) inhibit total antibiotic productivity overall. By adding industrial oils to medium containing L-valine the total production is comparable, giving unusual complex compositions.ConclusionsSince the cost and the quality of teicoplanin production depend mainly on the fermentation process, we developed a robust and scalable fermentation process by using an industrial medium in which a complex composition can be modulated by the combined addition of suitable precursors. This work was performed in the wild-type strain ATCC 31121, which has a clear genetic background. This is important for starting a rational improvement program and also helps to better control teicoplanin production during process and strain development.

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Section: Introductionmentioning

confidence: 99%

Actinoplanes teichomyceticus ATCC 31121 as a cell factory for producing teicoplanin

Taurino¹,

Frattini

Marcone

et al. 2011

Microb Cell Fact

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“…Following the advent of MRSA and penicillin resistant Streptococcus pneumoniae in the 1980s, interest in the glycopeptide class of antibiotics has been renewed (Levine, 2006;Schilling et al, 2011). Other glycopeptides which are used today are teicoplanin, a glycopeptide compound isolated from Actinoplanes teichomyceticus in the 1970s (Jung et al, 2009) and telavancin (technically part of the lipoglycopeptide class of antibiotics), a newly approved synthetic derivate of vancomycin (Damodaran et al, 2011).…”

Section: History and Mechanism Of Action Of Glycopeptide Antibioticsmentioning

confidence: 99%

Deliberations on the impact of antibiotic contamination on dissemination of antibiotic resistance genes in aquatic environments

Berglund¹

2014

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The great success of antibiotics in treating bacterial infectious diseases has been hampered by the increasing prevalence of antibiotic resistant bacteria. Not only does antibiotic resistance threaten to increase the difficulty in treating bacterial infectious diseases, but it could also make medical procedures such as routine surgery and organ transplantations very dangerous to perform. Traditionally, antibiotic resistance has been regarded as a strictly clinical problem and studies of the problem have mostly been restricted to a clinical milieu. Recently, non-clinical environments, and in particular aquatic environments, have been recognised as important factors in development and dissemination of antibiotic resistance. Elevated concentrations of antibiotics in an environment are likely to drive a selection pressure which favours resistant bacteria, and are also believed to promote horizontal gene transfer among the indigenous bacteria. Antibiotic resistance genes are often located on mobile genetic elements such as plasmids and integrons, which have the ability to disseminate among taxonomically unrelated species. The environmental bacteria can thus serve as both reservoirs for resistance and hot spots for the development of new antibiotic resistance determinants.There is still a lack of data pertaining to how high antibiotic concentrations are necessary to drive a selection pressure in aquatic environments. The aim of this thesis is to determine the effect of high and low concentrations of antibiotics on environmental bacterial communities from different aquatic environments. In the studies performed, antibiotics were measured using liquid chromatography-mass spectrometry. Bacterial diversity and evenness were assessed using molecular fingerprints obtained with 16S rRNA gene-based denaturing gradient gel electrophoresis, and antibiotic resistance genes and class 1 integrons were quantified using real-time PCR.Water and sediment samples were collected from different rivers and canals in Pakistan. The environments differed in anthropogenic exposure from undisturbed to heavily contaminated. A general trend could be observed of high concentrations of antibiotics correlating to elevated concentrations of antibiotic resistance genes and integrons. Extremely high concentrations of antibiotic resistance genes and integrons were found in the sediments downstream of an industrial drug formulation site, which likely correlated to the high load of antibiotics found in the water. Antibiotic and antibiotic resistance gene concentrations were also shown to increase downstream of Ravi river, which flows through Lahore, a city of more than 10 million inhabitants. Rivers not impacted by anthropogenic contamination were found to contain antibiotics and resistance gene concentrations of similar levels as in Europe and the U.S. Similar measurements were performed in the Swedish river Stångån. The concentrations of antibiotic resistance genes and class 1 VI integrons were shown to increase in the river after it had passed...

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“…TN is two- to four-fold more active than VM against both methicillin-susceptible and methicillin-resistant isolates of S. aureus with minimum inhibitory concentration (MIC90) typically in the range of 0.51 and 1-2 mg/L, respectively (7). Chitosan [β (1, 4) 2-amino-2-D-glucose] is a cationic biopolymer, produced by alkaline N-deacetylation of chitin, which is the constituting element of the shells of crustaceans such as crabs and shrimps (8, 9).…”

Section: Introductionmentioning

confidence: 99%

The Use of Natural Biopolymer of Chitosan as Biodegradable Beads for Local Antibiotic Delivery: Release Studies

Movaffagh

Ghodsi

Bazzaz

et al. 2013

Jundishapur J Nat Pharm Prod

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A B S T R A C T Background:Chitosan is a naturally occurring biopolymer which has been widely used in a variety of biomedical applications including local antibiotic delivery due to its excellent mechanical properties, biodegradability and biocompatibility. Beads are spherical, porous carriers which are prepared from various materials including chitosan. Objectives: The current study aimed to fabricate a new controlled delivery system for local anti-infective treatment and to study its release behavior. Materials and Methods: Twenty beads were prepared from 1% or 2% chitosan solutions and immersed in vancomycin (VM) or teicoplanin (TN) solutions. The antibiotic release kinetics was determined by linear regression analysis supposing first order kinetics. Results: Immersion for 3 h resulted in significant increase in the total TN release that differed from 0.5 h of immersion, except for the 1% beads immersed in VM. Increasing the chitosan concentration significantly increased the total release and antibiotic load of beads. The release of TN was more delayed compared to that of VM, which allowed a gradual release beyond 3 days. The half-life (mean ± SEM) of both types of TNcontaining beads was significantly extended for 3 h immersion in comparison to 0.5 h immersion (26.1 ± 5.9 vs 10.9 ± 1.0 and 17.0 ± 2.1 vs 5.1 ± 1.9; P < 0.001). However, neither increasing the chitosan concentration, nor immersion time did result in any significant increase in the release of VM. Conclusions:The current study demonstrated an improved control of TN release impregnated in beads. It can be concluded that chitosan beads might be considered as a novel carrier for TN delivery to infected bone for local anti-infective therapy.

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Biosynthesis, biotechnological production, and application of teicoplanin: current state and perspectives

Cited by 36 publications

References 101 publications

Actinoplanes teichomyceticus ATCC 31121 as a cell factory for producing teicoplanin

Actinoplanes teichomyceticus ATCC 31121 as a cell factory for producing teicoplanin

Deliberations on the impact of antibiotic contamination on dissemination of antibiotic resistance genes in aquatic environments

The Use of Natural Biopolymer of Chitosan as Biodegradable Beads for Local Antibiotic Delivery: Release Studies

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