Biosynthesis, characterization of magnetic iron oxide nanoparticles and evaluations of the cytotoxicity and DNA damage of human breast carcinoma cell lines

Abstract

Magnetic iron oxide nanoparticles (MNPs) were synthesized using Albizia adianthifolia leaf extract as reducing and protecting agent. Colour changing, UV-Vis spectrum, X-ray diffraction (XRD), Fourier transform infrared (FT-IR) spectroscopy and scanning electron microscopy (SEM) confirmed the biosynthesis and characterization of MNPs. The XRD pattern revealed that MNPs are crystalline in nature. FT-IR spectral analysis showed that MNPs was capped with plant constituents. From SEM analysis, the MNPs were generally found to be spherical in shape and the size was ranged 32-100 nm. Free radical scavenging potentials of the MNPs against DPPH were confirmed based on its stable anti-oxidant effects. The synthesized MNPs were used to capture Staphylococcus aureus under the magnetic field effect. Further, it was observed that the MNPs are able to exert cytotoxic effect towards human breast (AMJ-13) and (MCF-7) cancer cells. The anti-proliferative effect of this treatment is due to cell death and inducing apoptosis. Mitochondrial membrane potential, acridine orange-propidium iodide staining assays as well as single cell and DNA gel electrophoresis analyses indicated that MNPs induce cell death only by apoptosis. The findings of present study suggest that the MNPs might be used for medicinal applications particularly for cancer therapeutics.

Biosynthesis and Characterization of Iron Oxide Nanoparticles via Syzygium aromaticum Extract and Determination of Its Cytotoxicity against Human Breast Cancer Cell Lines

“…The XRD peaks of the samples was consistent with standard XRD pattern of Fe-O (No: 19-0629) which confirmed the crystalline of iron oxide nanoparticles. The finding result was also confirmed with previous report [32][33][34][35].…”

Catalytic degradation of methylene blue by iron nanoparticles synthesized using Galinsoga parviflora, Conyza bonariensis and Bidens pilosa leaf extracts

“…From the SEM micrographs, GpNPs, CbNPs and BpNPs nanoparticles were agglomerated due to presence of various biomolecules, chloride, and potassium compounds present in the leaf extracts which influence the morphology of the nanoparticles [14] and the gold used in sample preparation for SEM analysis [58]. Polyphenols present in the aqueous extracts of Conyza bonariensis, Bidens pilosa, and Galinsoga parviflora play a vital role as reducing agents and control the agglomeration of the nanoparticles through van der Waals interaction [48,59].…”

Albizia Adianthifolia: Botany, Medicinal Uses, Phytochemistry, and Pharmacological Properties

“…Over the years, pharmacological studies on A. adianthifolia extracts and compounds extracted from the species showed potent in vitro and in vivo pharmacological activities including acetylcholinesterase enzyme inhibitory [ 69 , 72 , 73 ], anthelmintic [ 70 , 74 ], antiamoebic [ 74 ], antibacterial [ 27 , 53 , 68 , 73 , 75 ], antimycobacterial [ 76 ], anti-sexually transmitted infections [ 77 ], antifungal [ 27 , 68 ], anti-inflammatory [ 73 , 78 ], antioxidant [ 27 , 28 , 69 , 79 , 80 ], anxiolytic and antidepressant [ 79 ], cognitive-enhancing [ 28 ], haemolytic [ 66 , 81 ], hypoglycemic and antihyperglycemic [ 45 ], immunomodulatory [ 66 ], cytotoxicity [ 77 , 80 – 84 ].…”

“…The increased activities of superoxide dismutase, glutathione peroxidase, catalase, and glutathione level and the decreased levels of protein carbonyl and malondialdehyde induced by administration of the aqueous extract of A. adianthifolia leaves implied that this plant extract possesses strong antioxidant property [ 79 ]. Sulaiman et al (2017) [ 80 ] evaluated the antioxidant activities of magnetic iron oxide nanoparticles synthesized using A. adianthifolia leaf extracts by using the DPPH free radical scavenging assay. The free radical scavenging potential of the magnetic iron oxide nanoparticles was confirmed based on its stable antioxidant effects [ 80 ].…”

“…Sulaiman et al (2017) [ 80 ] evaluated the antioxidant activities of magnetic iron oxide nanoparticles synthesized using A. adianthifolia leaf extracts by using the DPPH free radical scavenging assay. The free radical scavenging potential of the magnetic iron oxide nanoparticles was confirmed based on its stable antioxidant effects [ 80 ].…”