Biosynthesis of Cervimycin C, an Aromatic Polyketide Antibiotic Bearing an Unusual Dimethylmalonyl Moiety.

Isolation of the lysolipin gene cluster of Streptomyces tendae Tü 4042

“…It has been shown experimentally that the amidotransferase OxyD, a homologue of LlpA (54% amino acid identity), is necessary and sufficient to produce amidated polyketide precursors (Zhang et al, 2006). Another example of a similar starting mechanism was postulated for cervimycin biosynthesis (Herold et al, 2004).…”

Metabolic Engineering of Microorganisms for Biosynthesis of Antibiotics

“…In some cases, drug precursors themselves might also have other industrial or medicinal values, justifying their production on large scale. There are precursors of several antibiotics that include monensin (Vrijbloed et al 1999), cervimycin C (Herold et al 2004), and valanimycin (Garg et al 2008). There are engineered microbes for efficient production of antibiotics was given in Table 18.1.…”

Antibiotics

“…In essence, the labeled precursors are added to the growing culture of antibiotic-producing organism, which allows for their full or partial incorporation into the antibiotic molecule in the course of the biosynthesis. Antibiotic is then extracted, purified, and investigated with regard to incorporation of the labeled precursors (or parts thereof) by means of LC-MS/MS or NMR (48,49). In some cases, stereochemically labeled precursors shall be used to determine the biosynthetic origin of certain "building blocks" (50).…”