Biosynthesis of Fatty Acids in Obese Mice in Vivo. I. Studies with Glucose-1-3H(1-14C), Glucose-6-3H(6-14C), DL-Lactate-2-3H(2-14C), and Glycerol-2-3H(1,3-14C)*

Lamdin, Ezra; Oji, Nobuyoshi; Slavinski, R

doi:10.1021/bi00856a028

Cited by 30 publications

(30 citation statements)

References 18 publications

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“…Lactateformation in perfused mouse liver. Because of the increased rates of synthesis of liver lipid from glucose in obese-hyperglycaemic mice (Shigeta & Shreeve, 1964; Winand, Furnelle, Georis & Christophe, 1968;Jansen, Zanetti & Hutchison, 1967;Shreeve, Lamdin, Oji & Slavinski, 1967;Zomzely & Mayer, 1959;Lamdin, Shreeve, Slavinski & Oji, 1969), we assessed the capacity of the glycolytic pathway in liver. This capacity may be reflected in the rate of lactate release, perhaps especially in anaerobic conditions.…”

Section: Discussionmentioning

confidence: 99%

Carbohydrate metabolism of the isolated perfused liver of normal and genetically obese–hyperglycaemic (ob/ob) mice

Elliott

Hems

Beloff-Chain

1971

Biochemical Journal

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1. A technique for perfusion of the mouse liver has been developed, and aspects of carbohydrate metabolism have been investigated in the perfused liver of normal and genetically obese mice, homozygous for the recessive gene ob. 2. Rates of gluconeogenesis in perfused mouse liver were faster than those reported for slices of mouse liver, particularly from lactate and pyruvate. 3. The rate of glycogen breakdown to glucose, but not to lactate, was faster in liver from fed obese mice. 4. The capacity for glycogen synthesis from glucose was enhanced in liver from 20h-starved obese mice. 5. The capacity for gluconeogenesis from a number of substrates was not significantly altered in livers from fed or starved obese mice when compared with that of lean mice. 6. These results suggest that the liver contributes to the hyperglycaemia of the obese mice by increased glycogenolysis, and that liver glycogen in obese mice is maintained by synthesis from dietary glucose.

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Section: Discussionmentioning

confidence: 99%

Carbohydrate metabolism of the isolated perfused liver of normal and genetically obese–hyperglycaemic (ob/ob) mice

Elliott

Hems

Beloff-Chain

1971

Biochemical Journal

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“…These workers showed that tritium disappeared more rapidly than 14C from circulating glucose and it was reported that these differences were largely due to less recycling of tritium than 14C Schaeffer 1967, 1968;Katz and Dunn 1967;Hetenyi and Mak 1970). Formation of water appears to be the predominant early fate of the hydrogen from metabolized glucose (Katz and Dunn 1967;Shreeve et al 1967) and the dilution of tritium from glucose in a large pool of body water minimized recycling of this tracer to the glucose pool.…”

Section: Introductionmentioning

confidence: 99%

Estimation of the Total Entry Rate and Resynthesis of Glucose in Sheep Using Glucoses Uniformly Labelled With 14C and Variously Labelled With 3H

Judson¹,

Leng²

1972

Aust. Jnl. Of Bio. Sci.

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Comparisons have been made of [6_ 3H]-, [3-3H]-, and [2-3H]glucose with [U-14C]glucose for measuring parameters of glucose metabolism in sheep given their daily ration in 24 equal amounts at hourly intervals. The specific radioactivity (R) of plasma glucose was measured at frequent intervals from 0 to 10 hr and 0 to 32 hr after the start of a constant infusion. or single injection, of mixtures of these isotopes respectively.Estimates of glucose pool size by consideration of either the initial rectilinear slope (monoexponential analysis) or the multiexponential components of the log R-time curve after single injections of [3H} and [14C]glucose were similar.The mean irreversible loss of plasma glucose estimated by using [3-3H}, and [2-3H]glucose was approximately 79, 70, and 103 respectively. Further release of tritium to body water apparently occurred between 7 and 32 hr following injections of [6-3H} and [3-3H]glucose.Resynthesis of glucose from blood bicarbonate was equivalent to approximately 2% of the irreversible loss of glucose carbon, whereas resynthesis of carbonbound hydrogen of plasma glucose from body water was negligible, although about 91 % of this hydrogen was apparently derived from body water.It was suggested that 20-30% of the intermediates of glucose metabolism were utilized in synthetic reactions and that [2-3H]glucose with [U-14C]glucose was the most useful mixture of labelled glucoses for studies of glucose metabolism, particularly for estimating the extent of glucose resynthesis.

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“…In studying lipid metabolism the obese mutant, commonly referred to as the obese-hyperglycaemic syndrome, has been the subject of much research (Mayer, 1960;Jansen, Zanetti & Hutchison, 1967;Mayer & Thomas, 1967;Schreeve et al 1967). The primary alteration in gene product in this mutant is, however, not yet known.…”

Section: Introductionmentioning

confidence: 99%

Genetic control of metabolism: enzyme studies of theobeseandadiposemutants in the mouse

Bulfield

1972

Genet. Res.

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The activity of several enzymes has been determined in the livers of homozygous obese and adipose mice, their normal litter-mates, and phenocopies induced in normal mice by aurothioglucose (ATG) injections.Obese, adipose and ATG mice had higher activities of ATP citrate lyase, malic enzyme (NADP malate dehydrogenase) and pyruvate kinase than normal mice. Heterozygote activities are indistinguishable from wildtype. There was no difference between normal and fat litter-mates in the activity of malate dehydrogenase (NAD-linked), lactate dehydrogenase, isocitrate dehydrogenase and fumarase.Crosses between mice doubly heterozygous for both the ad and ob genes produced offspring that were only ' fat' or ' normal' and no offspring could be phenotypically recognized as the double mutant, either physically or in terms of ATP citrate lyase activity.Gas-liquid chromatography of the fatty acids of the depot fat showed no differences between any of the types of litter-mate.The alterations found in enzyme activity in obese and adipose mice are compared to several other enzyme activity differences reported in the literature for obese mice. These are discussed in relation to genetical criteria that may be established to assess, from quantitative data, whether an enzyme is the site of the primary lesion in a mutant phenotype. Some general observations are made on genetics and the control of metabolism.

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Biosynthesis of Fatty Acids in Obese Mice in Vivo. I. Studies with Glucose-1-³H(1-¹⁴C), Glucose-6-³H(6-¹⁴C), DL-Lactate-2-³H(2-¹⁴C), and Glycerol-2-³H(1,3-¹⁴C)^*

Cited by 30 publications

References 18 publications

Carbohydrate metabolism of the isolated perfused liver of normal and genetically obese–hyperglycaemic (ob/ob) mice

Carbohydrate metabolism of the isolated perfused liver of normal and genetically obese–hyperglycaemic (ob/ob) mice

Estimation of the Total Entry Rate and Resynthesis of Glucose in Sheep Using Glucoses Uniformly Labelled With 14C and Variously Labelled With 3H

Genetic control of metabolism: enzyme studies of theobeseandadiposemutants in the mouse

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