Biosynthesis of Flower-Shaped CuO Nanostructures and Their Photocatalytic and Antibacterial Activities

Abstract

HIGHLIGHTS• Eugenol (4-allyl-2-methoxyphenol) extracted from O. sanctum leaves is used as a natural reducing agent for the synthesis of CuO nanoflowers (NFs).• CuO-NFs can degrade methylene blue with an efficiency of 90%. • CuO-NFs offer a new vision to deactivate multi-drug microorganisms. A B S T R AC T C o pper oxide nanoflowers (CuO-NFs) have been synthesized through a novel green route using Tulsi leaves-extracted eugenol (4-allyl-2-methoxyphenol) as reducing agent. Characterizations results reveal the growth of crystalline singlephase CuO-NFs with monoclinic structure. The prepared CuO-NFs can effectively degrade methylene blue with 90% efficiency. They also show strong barrier against E. coli (27 ± 2 mm) at the concentration of 100 µg mL −1 , while at the concentration of 25 µg mL −1 weak barrier has been found against all examined bacterial organisms. The results provide important evidence that CuO-NFs have sustainable performance in methylene blue degradation as well as bacterial organisms.

Peroxidase-like activity and antimicrobial properties of curcumin-inorganic hybrid nanostructure

“…The free radicals formed as a result of the reaction described above cause oxidative damage to the bacterial membrane ( Baldemir et al, 2017 ). Similar to our findings, in another study, application of 25 µg / mL eugenol-Cu hNF has been reported to have an inhibitory effect against Escherichia coli , Staphylococcus aureus and Pseudomonas fluorescens strains ( Sidduqui et al, 2020 ). Celik et al (2020) reported that the catalytic and antimicrobial activity of NFs depends on the Fenton mechanism and the morphology of NFs.…”

Antibacterial and Antiviral Functional Materials: Chemistry and Biological Activity toward Tackling COVID-19-like Pandemics

“…Recently, Siddiqui et al synthesized copper oxide nanoflowers (CuO-NFs) and reported excellent performance for the degradation of methylene blue as well as inactivation of microorganisms ( E. coli ). 306 …”

Synthesis, microbial susceptibility and anti-cancerous properties of copper oxide nanoparticles- review

“…Flower shaped Copper Oxide Nanostructures obtained by Siddiqui et al [35] using O. sanctum and eugenol as capping agent exhibited Anti-microbial propensity against P vulgaris, P fluorescens, S. aureus and E. coli via agar diffusion method. E. coli exhibited maximum propensity when subjected to different concentrations ranging from 25 ug ml −1 to 100 ug ml −1 of Copper Oxide nanostructures using agar diffusion method.…”

“…Green synthesis of Copper Oxide Nanoparticles using CuCl 2 .2H 2 O (Cupric Chloride dihydrate) and aqueous leaf extract of Malva sylvestris along with sodium hydroxide yielded Copper Oxide Nanoparticles of 5 nm to 30 nm as analysed by SEM [25]. Floral extract of Magnolia champaca [26] and leaf extract of O. Sanctum [35] (Tulsi leaves) with Eugenol as capping and stabilizing agent was used to prepare Copper Oxide Nanoparticles from copper acetate as starting material. Copper Oxide Nanoparticles with average crystalline size of 35±6 nm was obtained using the floral extract of Magnolia champaca [26] and were studied not only for its toxicity effect using DPPH ABST Test, but also evaluated for toxicity on aquatic life form using zebra fish embryos as study model [26].…”

“…Flower shaped Copper Oxide Nanoparticles with particle size of 250 nm were obtained using O. sanctum leaf extract and eugenol, with eugenol playing major role in obtaining flower shaped structure. The Copper Oxide Nanoparticles thus obtained were further studied for photocatalytic and antimicrobial activity [35]. P Yugandhar et al [36] in their green synthesis used S. alternifolium stem bark extract to prepare Copper Oxide Nanoparticles from Copper Sulfate.…”