Peroxidase-like activity and antimicrobial properties of curcumin-inorganic hybrid nanostructure

Abstract

For the first time in this study, curcumin was utilized as an organic component reacting with Cu (II) ion (Cu 2+ ) as an inorganic component for fabrication of curcumin based Cu hybrid nanostructure (Cu-hNs). We also systematically examined the catalytic effect towards guaiacol and antimicrobial activities of Cu-hNs towards fish pathogen bacteria. For the characterization of Cu-hNs, Scanning Electron Microscopy (SEM), Energy Dispersive X-ray spectroscopy (EDX) and Fourier transform infrared spectrometry (FT-IR) analysis were used. We claimed that hydroxyl group might react with Cu 2+ in phosphate solution (PO 4 -3 ) to form Cu-hNs. However, more uniform and spherical Cu-hNs were not seen owing to absence of more reactive functional groups like amine and carboxyl groups on structure of curcumin. In addition to our findings, synthesis of Cu-hNs were carried out in the various pH values to evaluate the effect of pHs on formation of Cu-hNs. The Cu-hNs exhibited remarkable catalytic activity throught the Fenton reaction in the presence of hydrogen peroxide (H 2 O 2 ) and effective antimicrobial activities against Gram-positive/negative fish pathogen bacteria. In this study, cheap and efficient synthesis of nanoflowers (NFs) using plant extracts is proposed for biomedical applications rather than expensive molecules such as amino acids and DNA.

Peroxidase Mimicking of Binary Polyacrylonitrile-CuO Nanoflowers and the Application in Colorimetric Detection of H2O2 and Ascorbic Acid

“…The ideal supports should possess a well-dispersed microspherical structure, which can provide a great opportunity for adsorbing colorimetric substrates and growing catalytic sites. A recent encouraging breakthrough in the preparation of immobilized enzymes, called “enzyme nanoflowers,” which greatly enhanced activities, was discovered by Dr. Zare and co-workers and several researchers. − At present, some nanomaterials with peroxidase activity were prepared using organic molecules instead of enzymes and proteins. − To maximize the peroxide-like activity, a structure of three-dimensional multiple-layered polyacrylonitrile (PAN) nanoflower with well-dispersed nanomaterials could be beneficial. Copper oxide (CuO), with the advantages of strong chemical stability, low cost, high catalytic activity, and good adsorption of substrates, has been largely applied as a peroxidase mimic in the field of colorimetric biosensing.…”

Self‐Synthesized POM‐Chitosan Material with Enzyme Mimetic Activities for Antioxidant Applications

“…The SEM image was further used to determine the morphology of the hybrids synthesized, which is very important or significant in the catalytic and antimicrobial activity. [21,35] The SEM images of CS and PMo 9 V 3 /CS as shown in Figure 2 revealed different surface appearances. The SEM image of CS (Figure 2a) showed a smooth and spherical morphology.…”

Prospective Application of Nanoparticles Green Synthesized Using Medicinal Plant Extracts as Novel Nanomedicines

“… 4 Allicin from Allium sativum L. and curcumin from Curcuma longa L. were successfully used as organic components in the preparation of copper hybrid nanostructures. 73 , 74 In addition, heavy molecules such as proteins, glycoproteins and lipids were also found. However, there is evidence in the literature of the involvement of some flavonoids such as rutin, quercetin, etc as reducing or capping agents.…”