Biosynthesis of Fluorescent Silver Nanoparticles From Leea Coccinea Leaves and Their Antibacterial Potentialities Against Xanthomonas Phaseoli Pv Phaseoli

Abstract

The synthesis of Silver Nanoparticles (SNP) from plants is a simple, fast and environmentally safe route. In the present study, the aqueous extract of fresh leaves from Leea coccinea L. was evaluated as a possible source of reducing and stabilizing agents to obtain SNP. The synthesized SNP were characterized by spectroscopic techniques such as UV-Visible spectrophotometry and Fourier Transform Infrared spectroscopy (FTIR), Scanning Electron and Confocal microscopies and the antimicrobial activity was evaluated against to Xanthomonas phaseoli pv. phaseoli using the agar diffusion methods. The results showed that the evaluated extract was promising for the green synthesis of the SNP, which were visually identified by the formation of a dark brown complex and by the presence of a peak of maximum absorption at 470 nm in a UV-VIS spectrum. FTIR spectrum of SNP showed main characteristic signals of aromatic compounds, carboxylic group between others confirmed through phytochemical screening that evidenced the presence of flavonoids, phenols, leucoanthocyanidins, terpenes and steroids groups. Fluorescents SNP with high degree of agglomeration were visualized by microscopies technical used. A promissory antibacterial activity of SNP was demonstrated by a zone of inhibition of the microbial growing. These results suggest the need to go deeper in the physical-chemical characterization and kinetic studies and the biological evaluation to make possible the use of this vegetable source to future develop of antibacterial formulations to the bean seeds protection.

Multitherapeutic Efficacy of Curly Kale Extract Fabricated Biogenic Silver Nanoparticles

“…This consequence or result is similar to earlier reports. 2 , 16 , 17 , 72 A hypothesis on the possible mode of action of the BroL-AgNPs could be that when the NPs interacted with the bacteria, it might have bind to the bacterial cell membrane and could have resulted in the modification of the chemical and physical nature of the bacterial cell membrane resulting in the malfunction of the normal biological process such as permeability, respiration, etc. of cells leading to the death of the bacteria.…”