Biosynthesis of Heterocyclic Antibiotics in Actinomycetes and an Approach to Synthesize the Natural Compounds

Onaka, Hiroyasu

doi:10.3209/saj.20.62

Cited by 14 publications

(17 citation statements)

References 25 publications

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“…NRPS gene clusters have been detected recently in Lechevalieria species (Ayuso-Sacido and Genilloud 2005) that are involved in the synthesis of antitumor (Onaka 2006;Sanchez et al 2006) and antifungal (Lee et al 2004) compounds. Our own preliminary survey based on a comparison of gene sequence identities with those involved in the synthesis of known bioactive compounds promises that among those encoded by Atacama actinomycetes are novel NRP synthases.…”

Section: Discussionmentioning

confidence: 99%

Diversity of culturable actinomycetes in hyper-arid soils of the Atacama Desert, Chile

Okoro

Brown

Jones

et al. 2008

Antonie van Leeuwenhoek

200

177

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The Atacama Desert presents one of the most extreme environments on Earth and we report here the first extensive isolations of actinomycetes from soils at various locations within the Desert. The use of selective isolation procedures enabled actinomycetes to be recovered from arid, hyper-arid and even extreme hyper-arid environments in significant numbers and diversity. In some cases actinomycetes were the only culturable bacteria to be isolated under the conditions of this study. Phylogenetic analysis and some phenotypic characterisation revealed that the majority of isolates belonged to members of the genera Amycolatopsis, Lechevalieria and Streptomyces, a high proportion of which represent novel centres of taxonomic variation. The results of this study support the view that arid desert soils constitute a largely unexplored repository of novel bacteria, while the high incidence of non-ribosomal peptide synthase genes in our isolates recommend them as promising material in screening for new bioactive natural products.

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Section: Discussionmentioning

confidence: 99%

Diversity of culturable actinomycetes in hyper-arid soils of the Atacama Desert, Chile

Okoro

Brown

Jones

et al. 2008

Antonie van Leeuwenhoek

200

177

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“…204, 205 Putative membrane-associated proteases (GodB and GodC) cleave the modified precursor peptide (GodA), and an N-acetyltransferase (GodH) modifies the newly formed N-terminus. 206, 207 Transcription of the goadsporin BGC is controlled by GodR, an activator, 208 whose overexpression results in increased production of goadsporin, although the best titers were achieved through heterologous expression in S. lividans and co-culturing with Tsukamurella pulmonis . 208, 209 In order to prevent inhibition of its own growth, the BGC encodes the immunity protein GodI (see below).…”

Section: Linear Azole-containing Peptides (Laps)mentioning

confidence: 99%

YcaO-Dependent Posttranslational Amide Activation: Biosynthesis, Structure, and Function

et al. 2017

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With advances in sequencing technology, uncharacterized proteins and domains of unknown function (DUFs) are rapidly accumulating in sequence databases and offer an opportunity to discover new protein chemistry and reaction mechanisms. The focus of this review, the formerly enigmatic YcaO superfamily (DUF181), has been found to catalyze a unique phosphorylation of a ribosomal peptide backbone amide upon attack by different nucleophiles. Established nucleophiles are the side chains of Cys, Ser, and Thr which gives rise to azoline/azole biosynthesis in ribosomally synthesized and posttranslationally modified peptide (RiPP) natural products. However, much remains unknown about the potential for YcaO proteins to collaborate with other nucleophiles. Recent work suggests potential in forming thioamides, macroamidines, and possibly additional post-translational modifications. This review covers all knowledge through mid-2016 regarding the biosynthetic gene clusters (BGCs), natural products, functions, mechanisms, and applications of YcaO proteins and outlines likely future research directions for this protein superfamily.

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“…Interestingly, only K252c was reported to be accumulated in the ΔstaI mutant of Streptomyces sp. TP-A0274 [31]. Therefore, we proposed that inactivation of spcI led to accumulation of both TDP-sugar and ICZ ring, and further, spcG may use TDP- L -rhamnose as a sugar donor to catalyze its attachment onto the N -13 atom of K252c to afford K252d (Scheme 2).…”

Section: Resultsmentioning

confidence: 99%

Cloning, Characterization and Heterologous Expression of the Indolocarbazole Biosynthetic Gene Cluster from Marine-Derived Streptomyces sanyensis FMA

Cui

et al. 2013

Marine Drugs

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The indolocarbazole (ICZ) alkaloids have attracted much attention due to their unique structures and potential therapeutic applications. A series of ICZs were recently isolated and identified from a marine-derived actinomycete strain, Streptomyces sanyensis FMA. To elucidate the biosynthetic machinery associated with ICZs production in S. sanyensis FMA, PCR using degenerate primers was carried out to clone the FAD-dependent monooxygenase gene fragment for ICZ ring formation, which was used as a probe to isolate the 34.6-kb DNA region containing the spc gene cluster. Sequence analysis revealed genes for ICZ ring formation (spcO, D, P, C), sugar unit formation (spcA, B, E, K, J, I), glycosylation (spcN, G), methylation (spcMA, MB), as well as regulation (spcR). Their involvement in ICZ biosynthesis was confirmed by gene inactivation and heterologous expression in Streptomyces coelicolor M1152. This work represents the first cloning and characterization of an ICZ gene cluster isolated from a marine-derived actinomycete strain and would be helpful for thoroughly understanding the biosynthetic mechanism of ICZ glycosides.

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Biosynthesis of Heterocyclic Antibiotics in Actinomycetes and an Approach to Synthesize the Natural Compounds

Cited by 14 publications

References 25 publications

Diversity of culturable actinomycetes in hyper-arid soils of the Atacama Desert, Chile

Diversity of culturable actinomycetes in hyper-arid soils of the Atacama Desert, Chile

YcaO-Dependent Posttranslational Amide Activation: Biosynthesis, Structure, and Function

Cloning, Characterization and Heterologous Expression of the Indolocarbazole Biosynthetic Gene Cluster from Marine-Derived Streptomyces sanyensis FMA

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