Biosynthesis of lovastatin using agro-industrial wastes as carrier substrates

Javed, Sadia; Meraj, Munazzah; Mahmood, S.; Hameed, Arruje; Naz, Farah; Hassan, S A M; Irfan, Rao

doi:10.4314/tjpr.v16i2.1

Cited by 8 publications

(3 citation statements)

References 14 publications

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“…Therefore, on the basis of results observed on the bioassay plates and HPLC, it was considered that the strain A. flavus as a potential lovastatin producing candidate for further studies. In accordance with our results, A. flavus was considered as the best producer of lovastatin in addition to A. terreus and other fungi [5,[46][47][48]. Similar results have been reported by other investigators for different fungal species [49][50][51].…”

Section: Discussionsupporting

confidence: 92%

Purification and Cytotoxicity Study of Lovastatin From Soil Fungi

Balraj

Saibaba

Archunan

et al. 2017

Asian J Pharm Clin Res

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Objective: The objective of the present study is to evaluate the anticancer potential of lovastatin obtained from fungal source.Methods: About 15 fungal cultures were isolated from soil samples collected from Bharathiar University, India, and all are identified and characterized through microscopic characterization. Lovastatin producing capability was confirmed through bioassay against Saccharomyces cerevisiae, and the ability of selected fungus to produce lovastatin was further confirmed by high-performance liquid chromatography. Maximum lovastatin producing fungi were further selected for purification (overloaded elution chromatography) and characterization done using inhibition rate (IR). 5-diphenyl tetrazolium bromide (MTT) assay using A549 cell line was performed for antitumor activity evaluation.Results: Among the 15 isolates, Aspergillus flavus exhibited the maximum zone of inhibition (1.5 cm) against the test organism through solid-state fermentation. The resemblance in retention time (RT) of peak shown in chromatograms of standard lovastatin (RT=25.1 minutes) and sample (RT=25.1 minutes) were similar. This confirmed the presence of lovastatin in the selected fungal isolate (A. flavus). The presence of two functional groups in lovastatin C=O and O-H was confirmed by IR spectrum 50% of cell lysis was observed in MTT assay.Conclusion: Lovastatin obtained from soil fungi is capable of producing lovastatin in good proportions. Obtained fungal lovastatin exhibited significant antitumor activity against A549 cell line. Like other biological derivatives, lovastatin from soil fungi had greater potential in anticancer activity, and further biosynthetic pathway insights in their production can improve the yield which aid in large scale production.

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Section: Discussionsupporting

confidence: 92%

Purification and Cytotoxicity Study of Lovastatin From Soil Fungi

Balraj

Saibaba

Archunan

et al. 2017

Asian J Pharm Clin Res

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“…Mukhtar and Haq, 2008). All these findings indicated that the survivability of parent strain depended on the nature of the microorganism, treatment period and the type of mutagens (Javed et al, 2013).…”

Section: Ntg Mutagenesis Bioassaymentioning

confidence: 88%

“…Agro industrial residues are composed of complex polysaccharides that support the microbial growth for the production of useful products (enzymes, organic acids, drugs, etc.) (Javed et al, 2013). The antimicrobial resistance is presently an urgent focus of research and new bioactive compounds are necessary to combat the pathogens (Freire-Moran et al 2011).…”

Section: Introductionmentioning

confidence: 99%

Screening Of Antibiotic Producing Actinomycetes From The Sediments Of Undisturbed Forest Areas And Its Hyper Activity After Mutation

2016

IJACEBS

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Abstract-Wide and uncontrolled usage of antibiotics has made the pathogens to become resistant to currently used antibiotics. There is an urgent need for development of a new drug or a highly active molecule for controlling antibiotic resistant strains. In this study 32 strains of Actinomycetes were isolated and subjected to primary screening by giant colony method against Bacillus subtilis, Escherichia coli, Pseudomonas aeruginosa and Aeromonas hydrophilia. The secondary screening was carried out by fermentation process. Antibacterial activity was evaluated by well plate method. The extract of isolates was subjected to well plate method against pathogenic bacteria. B. subtilis and E. coli were highly inhibited by AK1 isolate. Other isolates showed limited inhibition of bacteria. The AK1 isolate was mutated by UV irradiation. The mutants differed from the wild parent in reduced growth rates, changes in the shape and size of the colony, sporulation level, antibiotic activity and variation in the color of the mycelium. The zone of inhibition was higher with Actinomycetes AK1 inoculated plates. The secondary metabolite production was enhanced by UV mutagenesis when compared to wild type. Actinomycetes may produce different molecule that can inhibit different types of pathogens, however efforts like strain development can be done to produce new bioactive components against multidrug resistant bacteria.

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