Biosynthesis of monoethylene glycol in Saccharomyces cerevisiae utilizing native glycolytic enzymes

Uranukul, Boonsom; Woolston, Benjamin M.; Fink, Gerald R.; Stephanopoulos, Gregory

doi:10.1016/j.ymben.2018.09.012

Cited by 28 publications

(17 citation statements)

References 51 publications

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“…This procedure, with a yield of 98%, could be considered a promising alternative for the synthesis of bio-EG. Other approaches, such as through glucose in Saccharomyces cerevisiae using glycolytic enzymes [53], through the synthesis of serine in an engineered pathway in E. coli [54], using synthesis gas through the Wood-Ljungdahl pathway of carbon fixation present in acetogenic bacterial species such as Moorella thermoacetica and Clostridium ljungdahlii [55], and through gaseous alkenes using a strain of E. coli [56] could be used in a similar way.…”

Section: Bio-based Polyethylene Terephthalate (Bio-pet)mentioning

confidence: 99%

Bio-Polyethylene (Bio-PE), Bio-Polypropylene (Bio-PP) and Bio-Poly(ethylene terephthalate) (Bio-PET): Recent Developments in Bio-Based Polymers Analogous to Petroleum-Derived Ones for Packaging and Engineering Applications

2020

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In recent year, there has been increasing concern about the growing amount of plastic waste coming from daily life. Different kinds of synthetic plastics are currently used for an extensive range of needs, but in order to reduce the impact of petroleum-based plastics and material waste, considerable attention has been focused on “green” plastics. In this paper, we present a broad review on the advances in the research and development of bio-based polymers analogous to petroleum-derived ones. The main interest for the development of bio-based materials is the strong public concern about waste, pollution and carbon footprint. The sustainability of those polymers, for general and specific applications, is driven by the great progress in the processing technologies that refine biomass feedstocks in order to obtain bio-based monomers that are used as building blocks. At the same time, thanks to the industrial progress, it is possible to obtain more versatile and specific chemical structures in order to synthetize polymers with ad-hoc tailored properties and functionalities, with engineering applications that include packaging but also durable and electronic goods. In particular, three types of polymers were described in this review: Bio-polyethylene (Bio-PE), bio-polypropylene (Bio-PP) and Bio-poly(ethylene terephthalate) (Bio-PET). The recent advances in their development in terms of processing technologies, product development and applications, as well as their advantages and disadvantages, are reported.

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Section: Bio-based Polyethylene Terephthalate (Bio-pet)mentioning

confidence: 99%

Bio-Polyethylene (Bio-PE), Bio-Polypropylene (Bio-PP) and Bio-Poly(ethylene terephthalate) (Bio-PET): Recent Developments in Bio-Based Polymers Analogous to Petroleum-Derived Ones for Packaging and Engineering Applications

2020

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“…A recent article by Uranukul et al ( 2018 ) described the application of R1P pathway for EG production in S. cerevisiae . The pathway was expressed in a D-xylose-utilizing strain that had been evolutionally engineered to have increased growth rates on D-xylose, and from which all copies of D-xylulose kinase genes had been deleted (adaptive evolution had resulted in multiple copies of P. stipitis XYL3 and endogenous XKS1 genes in the genome).…”

Section: Synthetic Pathways For Glycolic Acid and Ethylene Glycol Promentioning

confidence: 99%

“…The discovery of GA as a by-product of the EG-producing strains also indicates that this pathway could be modified for GA production in yeast. The authors speculate that for the endogenous pathway to work, the strain has to have sufficient D-xylose isomerase activity but no D-xylulose kinase activity (Uranukul et al 2018 ).…”

Section: Synthetic Pathways For Glycolic Acid and Ethylene Glycol Promentioning

confidence: 99%

Biotechnological production of glycolic acid and ethylene glycol: current state and perspectives

Salusjärvi

Havukainen

Koivistoinen

et al. 2019

Appl Microbiol Biotechnol

108

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Glycolic acid (GA) and ethylene glycol (EG) are versatile two-carbon organic chemicals used in multiple daily applications. GA and EG are currently produced by chemical synthesis, but their biotechnological production from renewable resources has received a substantial interest. Several different metabolic pathways by using genetically modified microorganisms, such as Escherichia coli , Corynebacterium glutamicum and yeast have been established for their production. As a result, the yield of GA and EG produced from sugars has been significantly improved. Here, we describe the recent advancement in metabolic engineering efforts focusing on metabolic pathways and engineering strategies used for GA and EG production.

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“…The engineered xylose pathway is not the only way of obtaining EG. It can also be produced from glucose in Saccharomyces cerevisiae using glycolytic enzymes [92] and via the synthesis of serine in an engineered pathway in E. coli [93]. Serine is transformed into ethanolamine by a plant serine decarboxylase.…”

Section: Biosynthesis Of Egmentioning

confidence: 99%

Genes for a Circular and Sustainable Bio-PET Economy

Salvador¹,

Abdulmutalib²,

González³

et al. 2019

Preprint

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Plastics have become an important environmental concern due to their durability and resistance to degradation. Out of all plastic materials, polyesters such as polyethylene terephthalate (PET) are amenable to biological degradation due to the action of microbial polyester hydrolases. The hydrolysis products obtained from PET can thereby be used for the synthesis of novel PET as well as becoming a potential carbon source for microorganisms. In addition, microorganisms and biomass can be used for the synthesis of the constituent monomers of PET from renewable sources. The combination of both biodegradation and biosynthesis would enable a completely circular bio-PET economy beyond the conventional recycling processes. Circular strategies like this could contribute to significantly decrease the environmental impact of our dependence on this polymer. Here we review the efforts made towards turning PET into a viable feedstock for microbial transformations. We highlight current bottlenecks in the degradation of the polymer and the metabolism of the monomers and we showcase fully biological or semisynthetic processes leading to the synthesis of PET from sustainable substrates.

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Biosynthesis of monoethylene glycol in Saccharomyces cerevisiae utilizing native glycolytic enzymes

Cited by 28 publications

References 51 publications

Bio-Polyethylene (Bio-PE), Bio-Polypropylene (Bio-PP) and Bio-Poly(ethylene terephthalate) (Bio-PET): Recent Developments in Bio-Based Polymers Analogous to Petroleum-Derived Ones for Packaging and Engineering Applications

Bio-Polyethylene (Bio-PE), Bio-Polypropylene (Bio-PP) and Bio-Poly(ethylene terephthalate) (Bio-PET): Recent Developments in Bio-Based Polymers Analogous to Petroleum-Derived Ones for Packaging and Engineering Applications

Biotechnological production of glycolic acid and ethylene glycol: current state and perspectives

Genes for a Circular and Sustainable Bio-PET Economy

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