Biosynthesis of nitric oxide from l-arginine

Moncada, Salvador; Palmer, Richard; Higgs, E. A.

doi:10.1016/0006-2952(89)90403-6

Cited by 1,221 publications

(303 citation statements)

References 51 publications

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“…The coefficients could be approximated by fitting the curve using a special programme which incorporates the equations [1] or [2] and is applied to the measuring points. These coefficients represent the reaction rate constants and the initial concentration at the time t = 0.…”

Section: Hplc Analysis and Sample Preparationmentioning

confidence: 99%

“…These coefficients represent the reaction rate constants and the initial concentration at the time t = 0. Contrary to equation [1] no available programme succeeded in calculating realistic coefficients for equation [2], thus the reaction rate constant k B could be estimated only by comparison of the position of the plotted points and the shape of the curve.…”

Section: Hplc Analysis and Sample Preparationmentioning

confidence: 99%

“…These curves can be calculated with equations [1] and [2] which is the result of integrating the kinetic equations [3] for substance A, and [4] for substance B (14, 15).…”

Section: Figurementioning

confidence: 99%

“…In some samples only TV^-nitro-L-arginine was added. Thus, equation [1] (in this case being valid for W°-nitro-L-arginine) could be used not only to estimate but also to calculate the constant.…”

Section: Arg(no)mentioning

confidence: 99%

“…Nitric oxide, also known as endothelium-derived relaxing factor, plays an important role as a regulator of cell functions and of communication in the body (1)(2)(3). It is produced in the body from the terminal guanidino nitrogen of I-arginine (3)(4)(5) and has a half life of only a few seconds.…”

Section: Introductionmentioning

confidence: 99%

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Kinetics of the Degradation of N ^GNitro-L-Arginine and Its Methyl Ester in Human Umbilical Vein Blood and Amniotic Fluid

Klein¹,

Dudenhausen²

1996

CCLM

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Summary:The kinetics of the degradation of the inhibitors of the nitric oxide synthesis, A/°-nitro-L-arginine methyl ester and A^-nitro-Zx-arginine, were examined in human amniotic fluid and umbilical vein blood. The reaction rate constants were calculated or estimated using the time-controlled concentration course of both substances. These concentrations were measured by high-performance liquid chromatography with two different separation systems: ion-exchange chromatography and ion-pair chromatography. Using this method, either A/°-nitro-L-arginine methyl ester and/or M^nitro-L-arginine were added to 18 samples of amniotic fluid, 33 samples of plasma and 21 samples of uncentrifuged umbilical vein blood samples; subsequently these samples were used for measurement. The degradation of the two individual study substances can be described by a uni-unimolecular two-step consecutive reaction. Thereby, M'-nitro-L-arginine methyl ester decomposes to A/°-nitro-L-arginine. Although 7V°-nitro-£-arginine decomposed further, the decomposition product could not be identified. The average of the reaction rate constants for A/°-nitro-L-arginine methyl ester/7V G -nitro-L-arginine was determined, yielding the following values: 0.032 h~V 0.00047h-1 in amniotic fluid, 0.029 rrVO.00384 h" 1 and 0.00074h" 1 in plasma and O.SOlrVO.OOOOO IT 1 in uncentrifuged umbilical vein blood. During the first hours after sampling, these reaction rate constants could be used to approximate the concentrations of M^-nitro-L-arginine methyl ester and 7V°-nitro-L-arginine at the time of sampling.

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Section: Hplc Analysis and Sample Preparationmentioning

confidence: 99%

Section: Hplc Analysis and Sample Preparationmentioning

confidence: 99%

“…These curves can be calculated with equations [1] and [2] which is the result of integrating the kinetic equations [3] for substance A, and [4] for substance B (14, 15).…”

Section: Figurementioning

confidence: 99%

Section: Arg(no)mentioning

confidence: 99%

Section: Introductionmentioning

confidence: 99%

See 3 more Smart Citations

Kinetics of the Degradation of N ^GNitro-L-Arginine and Its Methyl Ester in Human Umbilical Vein Blood and Amniotic Fluid

Klein¹,

Dudenhausen²

1996

CCLM

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Oxygen Radicals and Neuropsychiatric Illness

Lohr¹

1991

Arch Gen Psychiatry

223

100

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Decreased nitric oxide production in chronic viral hepatitis B and C

et al. 1997

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Nitric oxide is a free radical gas molecule which may be implicated in antiviral defense. However, there is no information about its possible role in chronic viral hepatitis B and C. In this study we have analyzed the serum levels of NO2- (as an index of nitric oxide generation) from patients with chronic viral hepatitis B and C and relationship of same with the response to interferon therapy. Serum samples were analysed from 61 patients with chronic hepatitis B, 60 patients with chronic hepatitis C, 11 with chronic liver disease of nonviral origin, and 23 healthy controls. Levels of NO2- were statistically higher in healthy controls (P < 0.001) than in patients with chronic liver disease. No relation was found between NO2- and viremia or response to interferon therapy in patients with chronic hepatitis B. In contrast in chronic hepatitis C, responder patients had significantly higher NO2- than nonresponders (P < 0.01). With respect to the relation between NO2- levels and liver damage, patients with cirrhosis had lower NO2- levels than the rest of the patients (P < 0.001). In conclusion, patients with chronic viral hepatitis have low serum NO2- levels.

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Biosynthesis of nitric oxide from l-arginine

Cited by 1,221 publications

References 51 publications

Kinetics of the Degradation of N ^GNitro-L-Arginine and Its Methyl Ester in Human Umbilical Vein Blood and Amniotic Fluid

Kinetics of the Degradation of N ^GNitro-L-Arginine and Its Methyl Ester in Human Umbilical Vein Blood and Amniotic Fluid

Oxygen Radicals and Neuropsychiatric Illness

Decreased nitric oxide production in chronic viral hepatitis B and C

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Biosynthesis of nitric oxide from l-arginine

Cited by 1,221 publications

References 51 publications

Kinetics of the Degradation of N G Nitro-L-Arginine and Its Methyl Ester in Human Umbilical Vein Blood and Amniotic Fluid

Kinetics of the Degradation of N G Nitro-L-Arginine and Its Methyl Ester in Human Umbilical Vein Blood and Amniotic Fluid

Oxygen Radicals and Neuropsychiatric Illness

Decreased nitric oxide production in chronic viral hepatitis B and C

Contact Info

Product

Resources

About

Kinetics of the Degradation of N ^GNitro-L-Arginine and Its Methyl Ester in Human Umbilical Vein Blood and Amniotic Fluid

Kinetics of the Degradation of N ^GNitro-L-Arginine and Its Methyl Ester in Human Umbilical Vein Blood and Amniotic Fluid