Biosynthesis of silver nanoparticle from leaf extract of Desmodium gangeticum (L.) DC. and its biomedical potential

Thirunavoukkarasu, M.; Balaji, Umapathi; Behera, Sujit Kumar; Panda, Prasanna Kumar; Mishra, B.K.

doi:10.1016/j.saa.2013.07.033

Cited by 122 publications

(53 citation statements)

References 14 publications

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“…A large number of plant leaf extracts like Cinnamomum camphora [16], Chenopodium album [17], Stevia rebaudiana [18], Murraya koenigii [19], Annona squamosal [20], Desmodium gangeticum [21], Pulicaria glutinosa [22], Brucea javanica [23], Artocarpus heterophyllus [24], Caesalpinia coriaria [25], Abutilon indicum [26], Rosa indica [27], Tephrosia tinctoria [28] have been employed by several researchers for the synthesis of silver nanoparticles.…”

Section: Introductionmentioning

confidence: 99%

Green synthesis of silver nanoparticles using phlomis leaf extract and investigation of their antibacterial activity

et al. 2016

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In recent years, the green synthesis of silver nanoparticles using various plant extracts has attracted great attention. This is because, these methods are simple, inexpensive and, eco-friendly. In this study, it was observed that silver ions were reduced by phlomis leaf extract after 5 min, leading to the formation of crystalline silver nanoparticles. Phlomis species is known as a rich source of flavonoids, phenylpropanoids and other phenolic compounds. The silver nanoparticles produced by the phlomis extract were characterized by different techniques including UV-vis spectrophotometry, X-ray diffraction, scanning electron microscopy (SEM), transmission electron microscopy (TEM), and FT-IR. The SEM and TEM results indicated that AgNPs were predominantly spherical in shape with an average particle size of 25 nm. In addition, the antibacterial activity of biologically synthesized nanopartilcles against Gram-positive (Staphyloccocus aureus and Bacillus cereus) and Gram-negative (Salmonella typhimurium and Escherichia coli) bacteria was proved. This study, therefore, showed that the phlomis leaf extract could be used for the green synthesis of silver nanoparticles with the appropriate antibacterial activity.

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Section: Introductionmentioning

confidence: 99%

Green synthesis of silver nanoparticles using phlomis leaf extract and investigation of their antibacterial activity

et al. 2016

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“…(Thirunavoukkarasu et al 2013) Citrus sinensis (Kaviya et al 2011), Mangifera indica (Philip 2011), Alternanthera dentata ) Murrayya koenigii , Magnolia kobus (Kim et al 2014), Malva parviflora (Mervat et al 2012) and Ocimum Tenuiflorum (Patil et al 2012a). Zizyphus xylopyrus (Family: Rhamnaceae) is found throughout northwestern India, Pakistan and China, a large, straggling shrub or a small tree, armed with spines.…”

Section: Introductionmentioning

confidence: 99%

Synthesis of silver nanoparticles using medicinal Zizyphus xylopyrus bark extract

et al. 2014

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In the present paper, biosynthesis of silver nanoparticles using Zizyphus xylopyrus bark extract is reported. Z. xylopyrus bark extract is efficiently used for the biosynthesis of silver nanoparticles. UV-Visible spectroscopy showed surface plasmon resonance peaks in the range 413-420 nm confirming the formation of silver nanoparticles. Different factors affecting the synthesis of silver nanoparticles like methodology for the preparation of extract, concentration of silver nitrate solution used for biosynthesis and initial pH of the reaction mixture were studied. The extract prepared with 10 mM AgNO 3 solution by reflux extraction method at optimum initial pH of 11, resulted in higher conversion of silver ions to silver nanoparticles as compared with those prepared by open heating or ultrasonication. SEM analysis showed that the biosynthesized nanoparticles are spherical in nature and ranged from 60 to 70 nm in size. EDX suggested that the silver nanoparticles must be capped by the organic components present in the plant extract. This simple process for the biosynthesis of silver nanoparticles using aqueous extract of Z. xylopyrus is a green technology without the usage of hazardous and toxic solvents and chemicals and hence is environment friendly. The process has several advantages with reference to cost, compatibility for its application in medical and drug delivery, as well as for large-scale commercial production.

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“…In biologically mediated nanoparticle synthesis, the microbial enzymes or plant phytochemicals having reducing or antioxidant properties are usually responsible for the reduction of metal ions into their respective metal nanoparticles (Keat et al 2015;Nath and Banerjee 2013;Chaturvedi and Verma 2015). Biosynthesis of silver nanoparticles using bacteria, fungi, and plants is already well documented (Durán et al 2011;Chaturvedi and Verma 2015;Ingle et al 2009;Thirunavoukkarasu et al 2013). In fungal community, various fungal strains have been well reported, as an efficient bio-factory for the synthesis of metal nanoparticles Jaidev and Narasimha 2010;Saravanan and Nanda 2010).…”

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confidence: 99%

Synergistic antibacterial and antibiofilm activity of silver nanoparticles biosynthesized by lignin-degrading fungus

Barapatre

Aadil

Jha

2016

Bioresour. Bioprocess.

140

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Background:The fabrication of silver nanoparticles (Ag-NPs) through green chemistry is an emerging area in the field of medical nanotechnology. Ag-NPs were fabricated by enzymatic reduction of AgNO 3 using two lignin-degrading fungus Aspergillus flavus (AfAg-NPs) and Emericella nidulans (EnAg-NPs). The prepared Ag-NPs were characterized by different spectroscopic techniques. Antibacterial activity of prepared Ag-NPs was demonstrated against selected Gram negative (Escherichia coli and Pseudomonas aeruginosa) and Gram positive (Staphylococcus aureus) bacteria in the term of minimum bactericidal concentration (MBC) and susceptibility constant (Z). The synergistic antibacterial activity of Ag-NPs with four conventional antibiotics was also determined by the fractional inhibitory concentration index (FICI) using the checkerboard microdilution method. The antibiofilm potential of Ag-NPs was also tested. Results:The plasmon surface resonance of biosynthesized Ag-NPs shows its characteristic peaks at UV and visible region (~450 and 280 nm). Fourier transform infrared spectrometer (FTIR) analysis confirms the nature of the capping agents as protein (enzyme) and indicates the role of protein (enzyme) in reduction of silver ions. The average particle size and charge of synthesized Ag-NPs was ~100 nm and ~−20 mV, respectively. X-ray diffraction (XRD) and TEM analysis confirmed the purity, shape, and size (quasi-spherical, hexagonal, and triangular) of Ag-NPs. Energy-dispersive X-ray spectroscopy (EDX) data validate the biological synthesis of Ag-NPs. Low MBC and high susceptibility constant indicate the high antimicrobial strength of biosynthesized Ag-NPs. The antibacterial analysis demonstrates the synergistic antimicrobial activity of Ag-NPs with antibiotics. This study also shows that biosynthesized Ag-NPs have ability to inhibit the biofilm formation by 80-90 %. Conclusion:The Aspergillus flavus and Emericella nidulans-mediated biosynthesized Ag-NPs have significant antimicrobial activity and demonstrate synergistic effect in combination with antibiotics. It suggests that nanoparticles can be effectively used in combination with antibiotics to improve the efficacy of antibiotics against pathogenic microbes. The substantial antibiofilm efficiency of biosynthesized Ag-NPs would also be helpful against sensitive and multidrug-resistant strains.

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Biosynthesis of silver nanoparticle from leaf extract of Desmodium gangeticum (L.) DC. and its biomedical potential

Cited by 122 publications

References 14 publications

Green synthesis of silver nanoparticles using phlomis leaf extract and investigation of their antibacterial activity

Green synthesis of silver nanoparticles using phlomis leaf extract and investigation of their antibacterial activity

Synthesis of silver nanoparticles using medicinal Zizyphus xylopyrus bark extract

Synergistic antibacterial and antibiofilm activity of silver nanoparticles biosynthesized by lignin-degrading fungus

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