Biosynthesis of silver nanoparticles by Novosphingobium sp. THG-C3 and their antimicrobial potential

Abstract

The present study described biosynthesis of silver nanoparticles (AgNPs) using a bacterial strain Novosphingobium sp. THG-C3, isolated from soil, and their application in antibacterial activity. The maximum absorbance values of the synthesized AgNPs was measured at 406 nm in ultraviolet-visible spectrophotometry and were mostly spherical in shape with particle size in range of 8-25 nm by field emission transmission electron microscopy analysis. X-ray diffraction pattern corresponding to planes (111), (200), (220), and (311) demonstrated the crystalline nature of the AgNPs. The synthesized AgNPs exhibited antimicrobial activity against various pathogens inculding Staphylococcus aureus, Candida tropicalis, Pseudomonas aeruginosa, Escherichia coli, Vibrio parahaemolyticus, Candida albicans, Salmonella enterica, Bacillus subtilis, and Bacillus cereus. In addition, the AgNPs in combination with commercial antibiotics enhanced antimicrobial activity against P. aeruginosa, S. enterica, E. coli, and V. parahaemolyticus. The AgNPs synthesized by strain Novosphingobium sp. THG-C3 are comparatively simple, green, cost-effective, and may serve as a potential antimicrobial agent.

Biosynthesis of silver nanoparticle from pomelo (Citrus Maxima) and their antibacterial activity against acidovorax oryzae RS-2

“…UV-vis spectroscopy After the addition of 2 mM and 4 mM silver nitrate solution, the colour of pomelo fruit extracts changed from colourless to reddish brown after heating at 60°C-80°C for 10 min (figure 1(a)), indicating the reduction of Ag ions by pomelo fruit extracts. As it is shown in figure 1(b), AgNPs showed maximum absorption at 426 nm by recording the surface plasmon resonance using UV-visible spectroscopy, which is consistent with previous studies [32][33][34]. Two different types of pomelo fruit extracts including PMY and PMR with 2 mM and 4 mM AgNO 3 solution, respectively, were determined for UV-vis spectroscopy.…”

Biologically rapid synthesis of silver nanoparticles by Sphingobium sp. MAH-11 T and their antibacterial activity and mechanisms investigation against drug-resistant pathogenic microbes

“…The average size of biosynthesized AgNPs was 11.9 nm. Previous studies similarly configured the size and shape of biosynthesized AgNPs [15]. The purity and composition of biosynthesized AgNPs were investigated using EDX spectrometer.…”

“…The accurate mechanism and manner of action behind the antimicrobial efficacy of AgNPs are still not well known. However, there are several proposed mechanisms for the action of nanoparticles, such as disturbance of the cell wall or cell membrane; alteration of nutrient uptake and electron transport, by damaging DNA and proteins or through the generation of reactive oxygen species (ROS), which lead to cell death [13][14][15]. The silver nanoparticle is becoming as an ideal candidate in the medical and biological platform due to its antibiofilm, antimicrobial, anticancer, anticoagulant and anti-inflammatory efficacies [3].…”

“…Recently several microorganisms, such as Brevibacterium frigoritolerans, Bacillus methylotrophicus and Novosphingobium sp. THG-C3 have been isolated for the biosynthesis of AgNPs [7,15,18]. In this study, Sphingobium sp.…”

Comparative Genomic Analysis Reveals Habitat-Specific Genes and Regulatory Hubs within the Genus Novosphingobium

“…Novosphingobium species have been isolated from a wide range of ecological habitats such as agricultural soil (2), pesticide-contaminated soil (3, 4), plant surfaces (5), and aquatic environments (6) (see Table 1). Previous studies have investigated Novosphingobium strains for their bioremediation capacity (7–10), nutrient cycling (11, 12), taxonomic characterization (3, 13), analysis of extracellular products (7), mutagenesis experiments on certain genes or gene clusters (14), disease conditions (15, 16), and application in nanoparticle formation for antibacterial activity (17). …”