Biosynthesis of silver nanoparticles using actinobacterium Streptomyces albogriseolus and its antibacterial activity

Abstract

An eco-friendly approach to the synthesis of silver nanoparticles (AgNPs) by extracellular components of Streptomyces albogriseolus has been reported. The isolated actinobacteria were genotypically identified by 16S rRNA sequencing analysis, and the morphology was observed by high-resolution scanning electron microscopy. The preliminary characterization of synthesized nanoparticles was carried out using ultraviolet-visible spectrophotometer. The maximum absorption spectra were found to be 409 nm at the 48th hour of incubation. The yield of AgNPs was found to be 72.64% as quantified by an atomic absorption spectrophotometer. The average size of AgNPs determined by the dynamic light scattering technique was 16.25 ± 1.6 nm. The results from transmission electron microscopy and X-ray diffraction confirmed the formation of spherical shaped and crystalline AgNPs. The interaction of protein with AgNPs was confirmed by Fourier transform infrared spectroscopy analysis. The biosynthesized AgNPs inhibited the growth of food pathogens (Bacillus cereus, Escherichia coli, and Staphylococcus aureus). Hence, the synthesis of AgNPs by S. albogriseolus could be employed as a probable antimicrobial agent to eliminate pathogenic microorganisms. This approach employed for the synthesis of nanoparticles paves a path for new biomaterial interfaces, which could be applied in different biomedical fields.

Antimicrobial activity of biogenic silver nanoparticles, and silver chloride nanoparticles: an overview and comments

“…Indeed, Candida albicans produced AgCl nanoparticles as shown by XRD pattern, together with Ag o nanoparticles, and the authors unfortunately did not discuss this pattern (Saminathan 2015). Kumar et al (2007) reported the synthesis of Ag°nanoparti-cles from lysozyme, although the XRD analysis confirmed the formation of the Ag°nanoparticles, the authors ignored the presence of AgCl nanoparticles, as revealed by XRD.…”

Synthesis of silver nanoparticles from two acidophilic strains of Pilimelia columellifera subsp. pallida and their antibacterial activities

“…Biosynthesis of silver nanoparticles (AgNPs) is an eco‐friendly approach by using different biological sources; for example, plants and microorganisms such as bacteria, fungi, and actinobacteria . There are previous reports on Streptomyces sp.—mediated synthesis of silver nanoparticles . Similarly, Narasimha et al successfully synthesized biological AgNPs and confirmed by observing the UV‐vis spectrum of treated cell filtrate of actinobacteria with silver nitrate at about 420 nm.…”

Green Nano Actinobacteriology – An Interdisciplinary Study

“…A considerable number of silver nanoparticles using Streptomyces sp. were reported to show antibacterial activity against varied human pathogens through extracellular mode [14,22,24,29,51,[53][54][55][56][57]. Unnamed actinomycetes mediated silver nanoparticles were also reported to produce antibacterial activity [13,18,44].…”