Biosynthesised AgCl NPs using Bacillus sp. 1/11 and evaluation of their cytotoxic activity and antibacterial and antibiofilm effects on multi‐drug resistant bacteria

Abstract

Silver nanoparticles (AgNPs) have attracted the attention of researchers due to their properties. Biological synthesis of AgNPs is eco-friendly and cost-effective preferred to physical and chemical methods, which utilize environmentally harmful agents and large amounts of energy. Microorganisms have been explored as potential biofactories to synthesize AgNPs. Bacterial NP synthesis is affected by Ag salt concentration, pH, temperature and bacterial species. In this study, spp., isolated from soil, were screened for AgNP synthesis at pH 12 with 5 mM Ag nitrate (AgNO) final concentration at room temperature. The isolate with fastest color change and the best ultraviolet-visible spectrum in width and height were chosen as premier one. AgNO and citrate salts were compared in terms of their influence on NP synthesis. Spherical Ag chloride (AgCl) NPs with a size range of 35-40 nm were synthesized in 1.5 mM Ag citrate solution. Fourier transform infrared analysis demonstrated that protein and carbohydrates were capping agents for NPs. In this study, antimicrobial and antitumor properties of the AgNP were investigated. The resulting AgCl NPs had bacteriostatic activity against four standard spp. And multi-drug resistant strain of . These NPs are also cytotoxic to cancer cell lines MCF-7, U87MG and T293.

Green synthesis of AgCl/Ag3PO4nanoparticle using cyanobacteria and assessment of its antibacterial, colorimetric detection of heavy metals and antioxidant properties

“…IBRC‐M5064 . This result, similar to the research done by Rezaii Somee et al [13] indicated that P. aeruginosa 48 is less resistant than the P. aeruginosa B52 against NPs. Although the mechanism of the antibacterial effect of AgNPs against bacteria is not well understood, however, some studies have shown that AgNPs may bind to cell membrane surfaces, thus their functions such as permeability and respiration, are impaired.…”

“…The solution incubated in a dark place at room temperature for 24 h. The progress of the reaction was observed by changing the colour of the solution [32]. Both NP colloids had the same concentration because the titration of these bacterial extracts by the Mohr method revealed that a total of 5 mM AgNO 3 changed to AgNP [13].…”

“…The biosynthesis of NP has a compatible production process with the environment, and its synthesis and stabilising are in one step [12]. As a green and eco‐friendly method, microorganisms like bacteria such as Bacillus [13], Myxobacteria virescens ( M . virescens ) [14] and Pseudomonas aeruginosa [15], fungi, such as Aspergillus [16] and Fusarium [17], microalgae [18] and cyanobacteria [19] and in addition plant extracts [20], plant root extract [21] and macroalgae [22] have been used for metal NPs synthesis.…”

Biosynthesis of Silver Nanoparticles Functionalized with Histidine and Phenylalanine Amino Acids for Potential Antioxidant and Antibacterial Activities

“…The appearance of a typical SPR peak between 400 and 500 nm further supported the color change, indicating that AgNPs were formed. As shown in Figure , the SPR peaks of AgNO 3 , LALE, and AgNP were 275, 301, and 433 nm, respectively.…”

“…The band gap of the synthesized silver nanoparticle from (Figure 2) was estimated to be about 2.45 eV, which is comparable to previously reported results. 49 The appearance of a typical SPR peak between 400 and 500 nm 50 further supported the color change, indicating that AgNPs were formed. As shown in Figure 2, the SPR peaks of AgNO 3 , LALE, and AgNP were 275, 301, and 433 nm, respectively.…”