Bacterial resistance remains a significant threat and a leading cause of death worldwide, despite massive attempts to control infections. In an effort to develop biologically active antibacterial and antifungal agents, six novel aryl-substituted-1,2,3-triazoles linked to carbohydrate units were synthesized through the Cu(I)-catalyzed azide-alkyne cycloaddition CuAAC of substituted-arylazides with a selection of alkyne-functionalized sugars. The chemical structures of the new derivatives were verified using different spectroscopic techniques. The novel clicked 1,2,3-triazoles were evaluated for in vitro antibacterial activity against Gram-positive Staphylococcus aureus and Gram-negative Pseudomonas aeruginosa, and the obtained results were compared with the activity of the reference antibiotic “Ampicillin”. Likewise, in vitro antifungal activity of the new 1,2,3-triazoles was investigated against Candida albicans and Aspergillus niger using “Nystatin” as a reference drug. The results of the biological evaluation pointed out that Staphylococcus aureus was more susceptible to all of the tested compounds than other examined microbes. In addition, some tested compounds exhibited promising antifungal activity.

Polyvinylpyrrolidone (PVP) is employed in several potential applications, relying of its special chemical and physical properties in addition to its low toxicity and biocompatibility. The aim of this work is to prepare polyvinylpyrrolidone-silver (PVP-Ag) nanocomposite with high inhibiting effect on the microbial growth and low cytotoxicity. In situ prepared small stable spherical silver nanoparticles, with narrow range particle size distribution, were obtained by easy, economical and rapid chemical reduction method. Silver ions were reduced to silver nanoparticles using low amount of sodium borohydride (NaBH4) as a strong reducing agent. PVP-Ag nanocomposite was prepared using PVP as a stabilizing and capping agent. Formation of the spherical silver nanoparticles with mean particle size 5 nm was confirmed by ultraviolet-visible spectroscopy, high resolution transmission electron microscopy, and dynamic light scattering. The inhibiting growth effect of the nanocomposite toward Gram-positive bacteria (Staphylococcus aureus), Gram-negative bacteria (Pseudomonas aeruginosa), and yeast fungus (Candida albicans) were studied. The cytotoxicity of the nanocomposite against BJ1 normal skin fibroblast cell line was tested. Results of this work presented perfect antimicrobial activity of the PVP-Ag nanocomposite towards bacteria and fungi with low cytotoxicity, which may lead to promising applications in skin wound healing.

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