Biosynthetic production of 13C and 14C erythronolide labeled erythromycin A

Walker, Alan E.; Pothuluri, Jairaj V.; Heinze, Thomas M.; Volmer, Dietrich A.; Cerniglia, Carl E.

doi:10.1002/(sici)1099-1344(199701)39:1<59::aid-jlcr946>3.3.co;2-f

Cited by 2 publications

(2 citation statements)

References 0 publications

Supporting

Mentioning

Contrasting

Order By: Relevance

“…Erythromycin A was purchased from Aldrich Chemical Co., Milwaukee, WI. [1,3,5,7,9,11,13‐ 14 C]erythromycin A (specific activity, 0.327 mCi mmol −1 ) was synthesized with purity >97%[12]. N ‐[ methyl ‐ 14 C]erythromycin A (specific activity, 54 mCi mmol −1 ) with a purity of 98% was purchased from American Radiolabeled Chemicals, St. Louis, MO.…”

Section: Methodsmentioning

confidence: 99%

Mineralization of erythromycin A in aquaculture sediments

Kim

Pak

Pothuluri

et al. 2004

FEMS Microbiology Letters

Self Cite

View full text Add to dashboard Cite

Mineralization of erythromycin A was studied using two differently (14)C-labeled erythromycins A, which were added to aquaculture sediment samples obtained from the two salmon hatchery sites in Washington state. The added erythromycin A did not significantly alter the numbers of the total viable colonies and erythromycin-resistant bacteria. Erythromycin-resistant Pseudomonas species contained a constitutive erythromycin esterase activity contributing to the inactivation of biologically active erythromycin A in aquatic and sediment environments. The initial rate of mineralization of erythromycin A appeared to be governed by the rate of release of soil-sorbed erythromycin A. After a prolonged lag time, the S-curves of erythromycin A mineralization were observed probably because of the increase in the population density metabolizing it. This study suggests that erythromycin A is partially or completely mineralized by the sediment microbial populations.

show abstract

Section: Methodsmentioning

confidence: 99%

Mineralization of erythromycin A in aquaculture sediments

Kim

Pak

Pothuluri

et al. 2004

FEMS Microbiology Letters

Self Cite

View full text Add to dashboard Cite

show abstract

“…Erythromycin A was purchased from Aldrich Chemical Co., Milwaukee, WI. [1,3,5,7,9,11,[13][14] C]erythromycin A (specific activity, 0.327 mCi mmol À1 ) was synthesized with purity >97% [12]. N-[methyl-14 C]erythromycin A (specific activity, 54 mCi mmol À1 ) with a purity of 98% was purchased from American Radiolabeled Chemicals, St. Louis, MO.…”

Section: Chemicalsmentioning

confidence: 99%