Biosynthetic studies with carbon-13: Effective use of a paramagnetic ion in the FT-13CNMR spectra of helicobasidin

On the absence of the glyceraldehyde 3-phosphate/pyruvate pathway for isoprenoid biosynthesis in fungi and yeasts

“…Radiolabeled MVA was incorporated by Gibberella fujikuroi into kaurene and gibberellins [20]. Such conclusions drawn from radiolabeling were confirmed by incorporation of 13 C‐labeled acetate, for instance, into the hemiterpenic moiety of tajixanthone from Aspergillus variecolor [21], the sesquiterpenes helicobasidin from Helicobasidium mompa [22] and PR toxin from Penicillium roqueforti [23], the sesquiterpenic moiety of polyketide‐terpenoid metabolites, such as andibenin from Aspergillus variecolor [24], austin from Aspergillus ustus [25] or sorokinianin from Bipolaris sorokiniana [26], the diterpenoid aphidicolin from Cephalosporium aphidicola [27] and finally triterpenoids, such as fusidic acid from Fusidium coccineum [28] and ergosterol in yeast [29].…”

Biosynthesis of sesquiterpenes

“…Additional experiments with [1-13C]-and [2-13C]acetates gave the expected specific enrichments of carbons 1, 3, 5, 7, 9, and 11 from [1-13C]acetate and of carbons 2, 4, 6, 8, 10, 12, 13, 14, and 15 from [2-13C]acetate. 274 Similar work on the biosynthesis of the trichothecane skeleton gave different results. 275 Initially the optimum incorporation time was determined using [2-14C]mevalonate (6).…”

On the absence of the glyceraldehyde 3-phosphate/pyruvate pathway for isoprenoid biosynthesis in fungi and yeasts

“…Radiolabeled MVA was incorporated by Gibberella fujikuroi into kaurene and gibberellins [20]. Such conclusions drawn from radiolabeling were con¢rmed by incorporation of IQ C-labeled acetate, for instance, into the hemiterpenic moiety of tajixanthone from Aspergillus variecolor [21], the sesquiterpenes helicobasidin from Helicobasidium mompa [22] and PR toxin from Penicillium roqueforti [23], the sesquiterpenic moiety of polyketide-terpenoid metabolites, such as andibenin from Aspergillus variecolor [24], austin from Aspergillus ustus [25] or sorokinianin from Bipolaris sorokiniana [26], the diterpenoid aphidicolin from Cephalosporium aphidicola [27] and ¢nally triterpenoids, such as fusidic acid from Fusidium coccineum [28] and ergosterol in yeast [29].…”