Biotechnological Advances and Trends in Engineering Trichoderma reesei towards Cellulase Hyperproducer

Fang, Hao; Li, Chaofeng; Zhao, Jiajia; Zhao, Chen

doi:10.1007/s12257-020-0243-y

Cited by 27 publications

(9 citation statements)

References 107 publications

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“…It is known that the efficiency of saccharification of lignocellulosic substrates is closely related to the performance of lignocellulases [ 11 ]. However, the composition of the enzyme complexes secreted by T. reesei may not be optimal for industrial applications due to the presence of certain enzymes in limiting amounts [ 9 , 46 ]. Optimization of enzyme complex with different proportions of cellulase components and accessory proteins has been employed for highly efficient bioconversion of lignocellulosic biomass [ 12 , 47 ].…”

Section: Discussionmentioning

confidence: 99%

“…The three components work synergistically to accomplish a complete deconstruction of cellulose [ 7 ]. Optimizing the composition of cellulase complexes by genetic engineering to strengthen the synergism has been proved to be practical [ 8 , 9 ], among which the overexpression of BGL or EGs is a powerful strategy for enhancing the synergistic effect [ 10 – 12 ]. Recently, Qian et al .…”

Section: Introductionmentioning

confidence: 99%

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Tailoring the expression of Xyr1 leads to efficient production of lignocellulolytic enzymes in Trichoderma reesei for improved saccharification of corncob residues

Shen

Yan

Wang

et al. 2022

Biotechnol Biofuels

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Background The filamentous fungus Trichoderma reesei is extensively used for the industrial-scale cellulase production. It has been well known that the transcription factor Xyr1 plays an important role in the regulatory network controlling cellulase gene expression. However, the role of Xyr1 in the regulation of cellulase expression has not been comprehensively elucidated, which hinders further improvement of lignocellulolytic enzyme production. Results Here, the expression dosage of xyr1 was tailored in T. reesei by differentially overexpressing the xyr1 gene under the control of three strong promoters (Pegl2, Pcbh1, and Pcdna1), and the transcript abundance of xyr1 was elevated 5.8-, 12.6-, and 47.2-fold, respectively. We found expression of cellulase genes was significantly increased in the Pegl2-driven xyr1 overexpression strain QE2X, whereas relatively low in the Pcbh1- and Pcdna1-driven overexpression strains. We also found that the Pegl2-driven overexpression of xyr1 caused a more significant opening of chromatin in the core promoter region of the prominent cellulase genes. Furthermore, the cellulase activity showed a 3.2-fold increase in the strain QE2X, while insignificant improvement in the Pcbh1- and Pcdna1-driven strains. Finally, the saccharification efficiency toward acid-pretreated corncob residues containing high-content lignin by the crude enzyme from QE2X was increased by 57.2% compared to that from the parental strain. Moreover, LC–MS/MS and RT-qPCR analysis revealed that expression of accessory proteins (Cip1, Cip2, Swo1, and LPMOs) was greatly improved in QE2X, which partly explained the promoting effect of the Pegl2-driven overexpression on enzymatic hydrolysis of lignocellulose biomass. Conclusions Our results underpin that the precise tailoring expression of xyr1 is essential for highly efficient cellulase synthesis, which provide new insights into the role of Xyr1 in regulating cellulase expression in T. reesei. Moreover, these results also provides a prospective strategy for strain improvement to enhance the lignocellulolytic enzyme production for use in biorefinery applications.

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Section: Discussionmentioning

confidence: 99%

Section: Introductionmentioning

confidence: 99%

Tailoring the expression of Xyr1 leads to efficient production of lignocellulolytic enzymes in Trichoderma reesei for improved saccharification of corncob residues

Shen

Yan

Wang

et al. 2022

Biotechnol Biofuels

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“…Diversity within the genus Trichoderma is evident from the wide range of phenotypes exhibited by the fungi as well as the various ecological roles and industrial purposes the fungi serve (Nakkeeran et al, 2021). Because of their various applications, different Trichoderma species have become model organisms for a broad spectrum of physiological phenomena, such as plant cell wall degradation and enzyme production (Fang et al, 2021), biocontrol (Zin and Badaluddin, 2020), and response to light (Schmoll, 2018). Within the genus Trichoderma , T. harzianum has been used as a commercial biocontrol agent against plant diseases (Fraceto et al, 2018).…”

Section: Discussionmentioning

confidence: 99%

Whole-Genome Sequencing and Comparative Genomic Analysis of Potential Biotechnological Strains of Trichoderma harzianum, Trichoderma atroviride, andTrichoderma reesei

Rosolen

Horta

Azevedo

et al. 2022

Preprint

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Fungi of the genus Trichoderma exhibit high genetic diversity and can thus be utilized in a large range of biotechnological applications. While Trichoderma reesei is the primary source of industrial enzymatic cocktails, Trichoderma atroviride and Trichoderma harzianum are widely used as commercial biocontrol agents against plant diseases. Recently, T. harzianum IOC-3844 (Th3844) and T. harzianum CBMAI-0179 (Th0179) demonstrated great potential in the enzymatic conversion of lignocellulose into fermentable sugars. Despite such potential, the genomes of both hydrolytic strains remain unclear. Herein, we performed whole-genome sequencing and assembly of Th3844 and Th0179 strains. To assess the genetic diversity within the genus Trichoderma, the results of both strains were compared with those from T. atroviride CBMAI-00020 (Ta0020) and T. reesei CBMAI-0711 (Tr0711). The resulting assembly revealed a total length of 40 Mb, (Th3844), 39 Mb (Th0179), 36 Mb (Ta0020), and 32 Mb (Tr0711), in which 10,786 (Th3844), 11,322 (Th0179), 10,082 (Ta0020), and 8,796 (Tr0711) genes were predicted. Then, the annotation of the predicted CDS sequences from the evaluated strain genes revealed 413 (Th3844), 413 (Th0179), 377 (Ta0020), and 329 (Tr0711) CAZymes. The orthology analysis revealed 18,349 orthogroups, which encompassed 95% of the total genes, 3,378 orthogroups with all species present, and 408 species-specific orthogroups. A genome-wide phylogenetic analysis modeled from the 1,864 single-copy orthogroups provided details on the relationship of the newly sequenced species with other Trichoderma and with more evolutionarily distant genera. Structural variants revealed genomic rearrangements between Th3844, Th0179, Ta0020, and Tr0711 with the T. reesei QM6a reference genome as well as the functional effects of such variants on the evaluated strains. In conclusion, the findings presented herein allow the genetic diversity of the evaluated strains, including those from the same species, to be viewed, offering opportunities to further explore such fungal genomes in future biotechnological and industrial applications.

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“…The U.S. Food and Drug Administration has approved using it for animal (fish) feed (Food & Drug Administration (FDA), 1993). It efficiently produces xylanases and cellulase-degrading enzymes in large quantities (Fang et al, 2021). Additionally, the degradation of date pits improves the chemical constituents and produces bioactive substances with added value for animal nutrition (Alyileili et al (2020).…”

Section: Oxidative Stress Antioxidant Statusmentioning

confidence: 99%

New Insights into the Effectiveness of Fungi-Degraded Date Pits Supplemented Diet on the Physiological and Biological Indices of Oreochromis niloticus Fingerlings

al.¹

2022

Egypt. J. of Aquatic Biolo. and Fish.

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Effect of Fungi-Degraded Date Pits Supplemented Diet on Oreochromis niloticus __________________________________________________________________________________ waste is expected to have several positive effects, such as improving water management, climate change, marine resources, terrestrial ecosystems, forestry, and biodiversity, among other things. __________________________________________________________________________________ Table 9. Effect of FDDP supplementation on biochemical analyses of O. niloticus Parameter D0 D1 D2 D3 D4 D5 D6 D7 D8 Glucose (mg/dl) 61.90±1.21 e 67.45±2.35 d 156.85±1.56 a 98.71±2.73 b 62.78±2.26 e 31.47±1.81 f 93.95±2.52 b 85.95±1.89 c 73.42±1.46 d HB (g/dl) 6.09± 0.13 f 6.11 ± 0.10 f 5.84± 0.18 g 6.77±0.01 d 9.80±0.51 b 7.11±0.27 c 6.30±0.02 f 10.18±0.07 a 6.54±0.13 e Uric acid (mg/dl) 9.65±0.91 d 17.41±1.01 b 12.10±0.31 e 15.94±1.45 b 9.65±0.61 d 19.18±2.4 4a 13.74±1.31 c 9.66±0.31 d 18.59±0.53 a Creatinine (mg/dl) 0.41± 0.04 e 0.54 ± 0.23 d 1.78± 0.21 a 1.30±0.41 b 0.23±0.04 f 0.85±0.27 c 1.22±0.16 b 0.24±0.06 f 0.49±0.03 d ALT (U/L) 67.01±1.91 d 99.31±2.19 a 80.68±1.36 c 62.82±1.96 c 31.20±1.25 g 87.73±1.61 b 81.35±0.95 c 37.75±2.01 f 79.33±1.43 c AST (U/L) 97.13± 1.73 d 148.08± 1.59 a 116.34±2.43 b 118.24±1.45 b 60.33± 1.03 f 101.82±1.26 cd 99.45±1.22 d 67.25±1.58 e 96.16±1 . 98 d

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Biotechnological Advances and Trends in Engineering Trichoderma reesei towards Cellulase Hyperproducer

Cited by 27 publications

References 107 publications

Tailoring the expression of Xyr1 leads to efficient production of lignocellulolytic enzymes in Trichoderma reesei for improved saccharification of corncob residues

Tailoring the expression of Xyr1 leads to efficient production of lignocellulolytic enzymes in Trichoderma reesei for improved saccharification of corncob residues

Whole-Genome Sequencing and Comparative Genomic Analysis of Potential Biotechnological Strains of Trichoderma harzianum, Trichoderma atroviride, andTrichoderma reesei

New Insights into the Effectiveness of Fungi-Degraded Date Pits Supplemented Diet on the Physiological and Biological Indices of Oreochromis niloticus Fingerlings

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