Biotechnological Advances in the Development of Outer Membrane Protein-Based Vaccines for Use in Aquaculture

Maiti, Biswajit; Chakraborty, Anirban; Karunasagar, Indrani

doi:10.1007/978-981-16-5195-3_3

Cited by 4 publications

(3 citation statements)

References 57 publications

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“…Commonly employed nanoparticles include polymeric nanoparticles such as alginate, chitosan, poly (lactic-co-glycolic acid) (PLGA), polylactic acid (PLA) dendrimers, and liposomes. However, conjugating the vaccine molecules with suitable nanocarriers becomes crucial in enhancing the properties of a vaccine and optimizing its delivery for effective response [47].…”

Section: Types Of Nanoparticles Used In Fish Vaccine Preparationmentioning

confidence: 99%

“…The effectiveness of the vaccine delivery method depends on factors such as the route of administration, pathogen type, fish life stage, and water parameters during the immunization process [79]. While nanoparticle-based vaccines have been extensively studied for response through the oral and parenteral routes, there is a research gap in understanding their applications in nasal, buccal, and topical delivery systems [47,80]. A list of vaccines designed to prevent A. hydrophila infection is provided in Table 1.…”

Section: Routes Of Vaccine Administrationmentioning

confidence: 99%

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Nanovaccines to Combat Aeromonas hydrophila Infections in Warm-Water Aquaculture: Opportunities and Challenges

Harshitha,

Nayak,

Disha

et al. 2023

Vaccines

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The application of nanotechnology in aquaculture for developing efficient vaccines has shown great potential in recent years. Nanovaccination, which involves encapsulating antigens of fish pathogens in various polymeric materials and nanoparticles, can afford protection to the antigens and a sustained release of the molecule. Oral administration of nanoparticles would be a convenient and cost-effective method for delivering vaccines in aquaculture while eliminating the need for stressful, labour-intensive injectables. The small size of nanoparticles allows them to overcome the degradative digestive enzymes and help deliver antigens to the target site of the fish more effectively. This targeted-delivery approach would help trigger cellular and humoral immune responses more efficiently, thereby enhancing the protective efficacy of vaccines. This is particularly relevant for combating diseases caused by pathogens like Aeromonas hydrophila, a major fish pathogen responsible for significant morbidity and mortality in the aquaculture sector. While the use of nanoparticle-based vaccines in aquaculture has shown promise, concerns exist about the potential toxicity associated with certain types of nanoparticles. Some nanoparticles have been found to exhibit varying degrees of toxicity, and their safety profiles need to be thoroughly assessed before widespread application. The introduction of nanovaccines has opened new vistas for improving aquaculture healthcare, but must be evaluated for potential toxicity before aquaculture applications. Details of nanovaccines and their mode of action, with a focus on protecting fish from infections and outbreaks caused by the ubiquitous opportunistic pathogen A. hydrophila, are reviewed here.

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Section: Types Of Nanoparticles Used In Fish Vaccine Preparationmentioning

confidence: 99%

Section: Routes Of Vaccine Administrationmentioning

confidence: 99%

Nanovaccines to Combat Aeromonas hydrophila Infections in Warm-Water Aquaculture: Opportunities and Challenges

Harshitha,

Nayak,

Disha

et al. 2023

Vaccines

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“…Outer membrane proteins are considered to be suitable vaccine candidates because they occur on the outer surface of the bacteria and contain exposed epitopes that are conserved among serovars (Maiti et al 2022). Principally, outer membrane proteins are prospective vaccine candidates because (1) they are highly immunogenic due to exposed epitopes on the surface, (2) they are highly conserved, and (3) they contain pathogen-associated molecular patterns that can be recognized by pathogen recognition receptors found on host immunogenic cells (Maiti et al 2020).…”

Section: Introductionmentioning

confidence: 99%

In silico evaluation of outer membrane protein S2 as a suitable vaccine candidate against Edwardsiella tarda infection of fish

Disha,

Yathisha,

Harshitha

et al. 2024

N American J Aquac

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Edwardsiella tarda is one of the primary emerging pathogens in fish aquaculture, responsible for Edwardsiellosis. The disease leads to significant economic loss to the farmers. Development of effective vaccines can minimise the disease burden. The type of vaccinations that are currently at the center of the most significant research are subunit vaccines. Outer membrane proteins (OMPs), being a component of the bacteria are very well known to be effective at stimulating immune responses in the host. In this study, the gene encoding for outer membrane protein S2 (OmpS2) of E. tarda was identified, cloned, and sequenced, followed by in silico analysis. The structure and sub‐cellular localization of the protein was first confirmed. Homology modelling of the whole protein was done and checked for its eligibility as a vaccine candidate. This was followed by identifying antigenic sites, B cell epitopes, and cytotoxic T‐lymphocyte epitopes on OmpS2. We obtained a few distinct vaccine peptides from the OmpS2. The complete genome of the fish pathogen E. tarda was subjected to genome analysis to identify potential epitopes that would bind to the fish major histocompatibility complex (MHC) molecule to elicit both humoral and cell‐mediated immune responses. This study provides valuable insights to consider OmpS2 as a potential vaccine candidate against E. tarda infection.

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Effect of sub-minimum inhibitory concentration of ceftriaxone on the expression of outer membrane proteins in Salmonella enterica serovar Typhi

Akshay

Anupama

Deekshit

et al. 2022

World J Microbiol Biotechnol

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Biotechnological Advances in the Development of Outer Membrane Protein-Based Vaccines for Use in Aquaculture

Cited by 4 publications

References 57 publications

Nanovaccines to Combat Aeromonas hydrophila Infections in Warm-Water Aquaculture: Opportunities and Challenges

Nanovaccines to Combat Aeromonas hydrophila Infections in Warm-Water Aquaculture: Opportunities and Challenges

In silico evaluation of outer membrane protein S2 as a suitable vaccine candidate against Edwardsiella tarda infection of fish

Effect of sub-minimum inhibitory concentration of ceftriaxone on the expression of outer membrane proteins in Salmonella enterica serovar Typhi

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