Biotechnological applications of bioluminescence and chemiluminescence

Roda, Aldo; Pasini, Patrizia; Mirasoli, Mara; Michelini, Elisa; Guardigli, Massimo

doi:10.1016/j.tibtech.2004.03.011

Cited by 315 publications

(166 citation statements)

References 67 publications

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“…Luminescent properties of semiconductor nanocrystals are usually inspected by photoluminescence (PL) produced using photoexcitation ) Qu and Peng, 2002(, electrochemiluminescence (ECL) generated by electron injection ) Zou and Ju, 2004( and cathodoluminescence given from electron impact ) Dabbousi et al, 1997(. In recent years, CL and relatedanalysis techniques have been utilized in different fields such as biology, bioimaging, biotechnology and analytical technology because of their widespread liner range, simple instrument and lack of background scattering light interference ) Roda et al, 2004(. Several advantages, including flexible photoexcitation, sharp photoemission, and excellent resistance to photobleaching have made them more attractive than conventional organic fluorophores as luminescent molecular probes (Parak et al, 2005).…”

Section: Introductionmentioning

confidence: 99%

Sensitive Determination of Cetirizine Using CdS Quantum dots as Oxidase Mimic-mediated Chemiluminescence of Sulfite

et al. 2016

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A new chemiluminescence (CL) method using cadmium sulfide quantum dots (QDs) as sensitizers is proposed for the chemiluminometric determination of cetirizine pharmaceutical formulation. CdS QDs were synthesized by using water soluble route. The nanoparticles were structurally and optically characterized by X-ray diffraction (XRD), Fourier transform infrared spectroscopy (FTIR), Ultra Violet-Visible (UVVis) absorption spectroscopy and scanning electron microscopy (SEM). In this study results shows that CdS quantum dots are enhancers of the weak CL emission. Trace amounts of cetirizine improved the sensitize effect of CdS quantum dots yielding a significant chemiluminescence enhancement of the Ce(IV)SO32?CdS QDs system. So, a new CL analysis system was selected for the determination of cetirizine. There is a good linear relationship between the relative chemiluminescence intensity and the concentration of cetirizine in the range of 1×10?9 1×10?6 molL?1 with a correlation coefficient (R2) of 0.9963 at the optimum conditions. The limit of detection (LOD) of this system was found to be 5×10?11 M. This method is simple, sensitive and cost effective, and also is accommodating for pharmaceutical applications.Kouhestany et al., International Current Pharmaceutical Journal, June 2016, 5(7): 59-62

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Section: Introductionmentioning

confidence: 99%

Sensitive Determination of Cetirizine Using CdS Quantum dots as Oxidase Mimic-mediated Chemiluminescence of Sulfite

et al. 2016

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“…They are the most sensitive and versatile reporter genes for bioanalytical purposes (1). Most studies have focused on firefly luciferases, many of which have been cloned and sequenced (2)(3)(4)(5)(6)(7)(8)(9)(10)(11).…”

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confidence: 99%

Active-Site Properties of Phrixotrix Railroad Worm Green and Red Bioluminescence-Eliciting Luciferases

Viviani¹,

Arnoldi²,

Balan³

et al. 2006

The Journal of Biochemistry

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The luciferases of the railroad worm Phrixotrix (Coleoptera: Phengodidae) are the only beetle luciferases that naturally produce true red bioluminescence. Previously, we cloned the green-(PxGR) and red-emitting (PxRE) luciferases of railroad worms Phrixotrix viviani and P. hirtus [OLE1]. These luciferases were expressed and purified, and their active-site properties were determined. The red-emitting PxRE luciferase displays flash-like kinetics, whereas PxGR luciferase displays slow-type kinetics. The substrate affinities and catalytic efficiency of PxRE luciferase are also higher than those of PxGR luciferase. Fluorescence studies with 8-anilino-1-naphthalene sulfonic acid and 6-p-toluidino-2-naphthalene sulfonic acid showed that the PxRE luciferase luciferinbinding site is more polar than that of PxGR luciferase, and it is sensitive to guanidine. Mutagenesis and modelling studies suggest that several invariant residues in the putative luciferin-binding site of PxRE luciferase cannot interact with excited oxyluciferin. These results suggest that one portion of the luciferin-binding site of the redemitting luciferase is tighter than that of PxGR luciferase, whereas the other portion could be more open and polar.

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“…Bioreporter bioluminescence has been detected by a number of different types of optical transducers, including photomultiplier tubes, photodiodes, microchannel plates, photographic films and charge-coupled devices (Simpson et al 1998). Bioluminescence and chemiluminescence applications in biotechnology using PMT or CCD imager were reviewed by Roda et al (2004). A biosensor consisting of a bioluminescent bioreporter (Pseudomonas fluorescence 5RL), an optical transducer (photomultiplier tube), and a light-tight chamber housing was used for evaluation of the antimicrobial efficacy of chlorine oxide (del Busto-Ramos et al 2008).…”

Section: Detectors Of Fiber Optic Biosensorsmentioning

confidence: 99%

Optical biosensors for food quality and safety assurance—a review

et al. 2011

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Food quality and safety is a scientific discipline describing handling, preparation and storage of food in ways that prevent food borne illness. Food serves as a growth medium for microorganisms that can be pathogenic or cause food spoilage. Therefore, it is imperative to have stringent laws and standards for the preparation, packaging and transportation of food. The conventional methods for detection of food contamination based on culturing, colony counting, chromatography and immunoassay are tedious and time consuming while biosensors have overcome some of these disadvantages. There is growing interest in biosensors due to high specificity, convenience and quick response. Optical biosensors show greater potential for the detection of pathogens, pesticide and drug residues, hygiene monitoring, heavy metals and other toxic substances in the food to check whether it is safe for consumption or not. This review focuses on optical biosensors, the recent developments in the associated instrumentation with emphasis on fiber optic and surface plasmon resonance (SPR) based biosensors for detecting a range of analytes in food samples, the major advantages and challenges associated with optical biosensors. It also briefly covers the different methods employed for the immobilization of bio-molecules used in developing biosensors.

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Biotechnological applications of bioluminescence and chemiluminescence

Cited by 315 publications

References 67 publications

Sensitive Determination of Cetirizine Using CdS Quantum dots as Oxidase Mimic-mediated Chemiluminescence of Sulfite

Sensitive Determination of Cetirizine Using CdS Quantum dots as Oxidase Mimic-mediated Chemiluminescence of Sulfite

Active-Site Properties of Phrixotrix Railroad Worm Green and Red Bioluminescence-Eliciting Luciferases

Optical biosensors for food quality and safety assurance—a review

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