Biotechnological Potential of Brewers Spent Grain and its Recent Applications

Xiros, Charilaos; Christakopoulos, Paul

doi:10.1007/s12649-012-9108-8

Cited by 114 publications

(89 citation statements)

References 90 publications

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“…Phenolic acids are the main antioxidant compounds present in cereals and, with up to 8 mg of hydroxycinnamic acids per gram dry matter BSG, its potential to increase the antioxidant capacity in food products has been investigated; however, results are not conclusive (2). In the preparation of an extruded snack product Ainsworth et al (62) found that BSG inclusion did not lead to any increase in total phenolic compounds or antioxidant capacity, a result which was also found in follow-up studies (63,64).…”

Section: Food Applications Of Bsgmentioning

confidence: 99%

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Brewers' spent grain: a review with an emphasis on food and health

2016

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Brewers' spent grain (BSG) is the most abundant by‐product generated in the beer‐brewing process. This material consists of the barley grain husks obtained as solid residue after the production of wort. BSG is rich in fibre and protein and, to date, the main use for the elimination of this by‐product has been as an animal feed. However, because of its nutritional content, BSG is of interest for application and fortification of human food products, particularly in view of its low cost and availability in large amounts. In addition, the importance of BSG as an ingredient and potential source of health‐promoting bioactive components is beginning to be recognised. The investigation of alternative uses of BSG is pertinent, not only from the perspective of the brewer who can benefit from valorisation of this by‐product, but also from an environmental perspective as the recycling and re‐use of industrial wastes and by‐products has become increasingly important. This review presents the current knowledge on BSG, covering its production, composition and methods for the release of valuable components, and focuses on the potential health benefits attributed to its constituents and the use of this brewer by‐product in food applications. Copyright © 2016 The Institute of Brewing & Distilling

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Section: Food Applications Of Bsgmentioning

confidence: 99%

“…BSG is basically a lignocellulosic material, the major constituents of which are fibre (hemicellulose and cellulose), protein and lignin ( Fig. 2) (2,4,10). Figure 3 shows the typical structure of lignocellulosic material.…”

Section: Bsg Compositionmentioning

confidence: 99%

Brewers' spent grain: a review with an emphasis on food and health

2016

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“…This iterative procedure decreases the concentration of adherent components of the BSG. At the same time, the concentrations in the wort kettle are reduced by dilution [31,32]. BSG liquor can be considered as the liquid product of a final lautering step, which contains lower concentrations of all components than the final wort.…”

Section: Analysis Of Bsg Liquormentioning

confidence: 99%

Brewers’ spent grain liquor as a feedstock for lactate production with Lactobacillus delbrueckii subsp. lactis

Akermann

Weiermüller

Christmann

et al. 2020

Engineering in Life Sciences

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Brewers' spent grain (BSG) is a low-cost by-product of the brewing process. BSG liquor names the liquid components of BSG, mainly glucose, maltose, and long-chain -1,4-glycosidic bond glucose oligomers. These substances should be separated in existing BSG biorefineries, as they might lead to an increased formation of microbeinhibiting compounds in well-established hydrothermal/enzymatic saccharification processes. In most cases, this liquid fraction is discarded. The present study presents for the first time an optimized process with BSG liquor for the purpose of producing bulk chemicals (e.g., lactate) in relevant concentrations. The process comprises the application of yeast extract, produced from own brewing processes, as the sole supplemented complex constituent in a simultaneous fermentation and saccharification process. Kinetic parameters for the final optimized process conditions with the organism Lactobacillus delbrueckii subsp. lactis were: maximum specific growth rate µ max = 0.47 h −1 , maximum lactate concentration c Lac, max = 79.06 g L −1 , process yield Y PS = 0.89 g Lac g Sugar −1 , lactate production rate q P = 4.18 g Lac g CDW −1 h −1 , and productivity P 15 h = 4.93 g Lac L −1 h −1 . BSG liquor, linked with yeast extract from Brewers' yeast, can be a promising substrate for further bioprocess engineering tasks and contribute to a holistic and sustainable usage of Brewers' spent grain. K E Y W O R D S biorefinery, brewers' spent grain (BSG) liquor, fermentation, lactate, Lactobacillus delbrueckii subsp. lactis 1 INTRODUCTION Brewers' spent grain (BSG) is the most abundant by-product of the brewing process and available in large quantities since the worldwide annual beer production was 1.95 million hL in 2017 [1]. As 0.2 kg of BSG accumulate from the production of 1 L beer [2], this resulted in about 39 million metric tons Abbreviations: BSG, brewers' spent grain; CDW, cell dry weight; MRS medium, cultivation medium by De Man, Rogosa, Sharpe.This is an open access article under the terms of the Creative Commons Attribution-NonCommercial-NoDerivs License, which permits use and distribution in any medium, provided the original work is properly cited, the use is non-commercial and no modifications or adaptations are made.

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“…Among them, BSG, owing to its distinct composition and food‐grade quality proved to be of special interest in terms of industrially relevant biotechnological processes. Diverse technologies have been developed to provide selective recovery of high‐value components from BSG, which in general refer to processing of carbohydrate, protein and lipid fraction of the substrate …”

Section: Introductionmentioning

confidence: 99%

“…Numerous technologies for production of amylases, amyloglucosidases, cellulases, hemicellulases or laccases, are described in literature. These applications are, however, dominated by filamentous fungi of Aspergillus , Penicillium , Fusarium or Trichoderma genera . Bacteria from the genus Bacillus are known producers of polysaccharide‐degrading enzymes that, besides peptidases, have significant industrial importance.…”

Section: Introductionmentioning

confidence: 99%

Evaluation of brewer's spent grain as a substrate for production of hydrolytic enzymes by keratinolytic bacteria

Łaba

Piegza

Kawa-Rygielska

2016

J of Chemical Tech & Biotech

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BACKGROUND Brewer's spent grain (BSG) is the most abundant by‐product of the brewery industry. The current massive output of BSG requires novel methods of bioconversion into added‐value products. Its composition and high biological value, i.e. high protein content, allows for various biotechnological applications. RESULTS Here, BSG was used as a substrate for submerged cultivation of keratinolytic bacteria, Bacillus cereus PCM 2849 and B. subtilis PCM 2850. The strains biosynthesized proteases to decompose proteins within the substrate and amylases to degrade residual starch. Enzymes degrading structural polysaccharides were also present. Bacillus cereus produced multiple proteases with high activity, predominantly with molecular weight (mw) >70 kDa, depending on the presence of minerals. Bacillus subtilis biosynthesised proteases of wide mw range, independently of the mineral components. Crude culture fluids of both bacteria exhibited proteolytic activity on a variety of proteins, including keratins. Structural deterioration of the BSG in microbial cultures was observed. CONCLUSION The BSG was a suitable medium component for the production of bacterial proteases and polysaccharide‐degrading enzymes. Two keratinolytic Bacilli, grown in the presence of BSG utilized residual proteins and biosynthesized proteases exhibiting keratinolytic activity. Bacillus cereus dominated in terms of protease production, while B. subtilis presented higher production of amylases, cellulases and xylanases. © 2016 Society of Chemical Industry

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Biotechnological Potential of Brewers Spent Grain and its Recent Applications

Cited by 114 publications

References 90 publications

Brewers' spent grain: a review with an emphasis on food and health

Brewers' spent grain: a review with an emphasis on food and health

Brewers’ spent grain liquor as a feedstock for lactate production with Lactobacillus delbrueckii subsp. lactis

Evaluation of brewer's spent grain as a substrate for production of hydrolytic enzymes by keratinolytic bacteria

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