Biotechnological valorization of oils from agro-industrial wastes to produce lipase using Aspergillus sp. from Amazon

Tacin, Mariana Vendrasco; Massi, Fernanda Pelisson; Fungaro, Maria Helena Pelegrinelli; Teixeira, María Francisca Simas; Paula, Ariela Veloso de; Santos-Ebinuma, Valéria de Carvalho

doi:10.1016/j.bcab.2018.11.013

Cited by 33 publications

(23 citation statements)

References 45 publications

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“…The results were similar to those of A. niger strain MTCC 872 [55] and A. niger [56]. According to the literature, several microbial lipases produced by Aspergillus species exhibit optimal temperature close to 37 • C [51,54,57,58].…”

Section: Temperature Effects On Enzyme Activity and Stabilitysupporting

confidence: 84%

Utilization of Clay Materials as Support for Aspergillus japonicus Lipase: An Eco-Friendly Approach

et al. 2021

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Lipase is an important group of biocatalysts, which combines versatility and specificity, and can catalyze several reactions when applied in a high amount of industrial processes. In this study, the lipase produced by Aspergillus japonicus under submerged cultivation, was immobilized by physical adsorption, using clay supports, namely, diatomite, vermiculite, montmorillonite KSF (MKSF) and kaolinite. Besides, the immobilized and free enzyme was characterized, regarding pH, temperature and kinetic parameters. The most promising clay support was MKSF that presented 69.47% immobilization yield and hydrolytic activity higher than the other conditions studied (270.7 U g−1). The derivative produced with MKSF showed high stability at pH and temperature, keeping 100% of its activity throughout 12 h of incubation in the pH ranges between 4.0 and 9.0 and at a temperature from 30 to 50 °C. In addition, the immobilized lipase on MKSF support showed an improvement in the catalytic performance. The study shows the potential of using clays as support to immobilized lipolytic enzymes by adsorption method, which is a simple and cost-effective process.

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Section: Temperature Effects On Enzyme Activity and Stabilitysupporting

confidence: 84%

Utilization of Clay Materials as Support for Aspergillus japonicus Lipase: An Eco-Friendly Approach

et al. 2021

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“…Outros autores que também conseguiram obter a enzima hidrolítica a partir da mesma amostra de forma satisfatória utilizaram resíduos agroindustriais em seu meio de produção. O uso de resíduos contribui para a biossíntese de baixo custo, pois são rejeitos da extensa produção agrícola de países em desenvolvimento e o seu descarte é também um problema ambiental, e contribuem principalmente no fornercimento de nutrientes necessários para o crescimento das células microbianas (NEMA, PATNALA, MANDARI et al, 2019;TACIN, MASSI, FUNGARO et al, 2019;PRABANINGTYAS, PUTRI, UTAMI et al, 2018). Kumar, Rani, Gunaseeli et al (2017) utilizaram em seus estudos a fermentação sólida e submersa para produção da lipase pelo Aspergillus japonicus MF-1: óleo de amendoim associado a agentes redutores e oxidantes na fermentação sólida, que obteve o máximo de produção da enzima (266 U/g), enquanto que em fermentação submersa na presença de óleo medicinal sendo produzido cerca de 58% deste valor.…”

Section: Resultsunclassified

Detecção De Esterase E Lipase Produzidas Por Fungos Filamentosos Isolados De Solos Da Caatinga / Detection of Esterase and Lipase Produced by Filamentous Fungi Isolated of Caatinga Soil

França

Marinho

Cândido

et al. 2020

BJD

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RESUMO As esterases (E.C. 3.1.1.1) são enzimas pertencentes à classe das carboxil ester hidrolases, e atuam diretamente na clivagem e na formação de ligações ésteres, enquanto as lipases (EC 3.1.1.3) são hidrolases que catalisam a hidrólise de triacilgliceróis em ácidos graxos livres e glicerol. A utilização de enzimas nas indústrias permite o desenvolvimento de processos tecnológicos tão eficientes quanto os realizados pela natureza e sem impactos ambientais. Nesse contexto, o objetivo deste estudo foi identificar micro-organismos isoaldos da Caatinga com potencial promissor na produção das enzimas esterase e lipase. Amostras de fungos filamentosos (6 amostras) do gênero Aspergillus sp. denominados de SIS (10, 11, 12, 14, 92, 112) e 2 isolados de Penicillium sp. denominados de SIS (21, 23) foram cultivados em meios de cultura específico utilizando as temperaturas de 28°C e 35°C, durante 72 h, com acompanhamento diário. A presença do halo característico evidenciou a presença das enzimas testadas. Os melhores resultados obtidos indicaram que a produção de lipase e esterase com o Penicillium sp. (SIS 23), apresentando índice enzimático de 2,0cm à 35°C e á 28°C e 1,5 cm após 72 horas de cultivo destacando-se como um potencial produtor de esterase e lipase. Palavras-chave: detecção de enzimas microbianas, fungos filamentosos, enzimas hidrolíticas ABCTRACT Esterases (E.C. 3.1.1.1) are enzymes belong to the class of carboxyl ester hydrolases and act directly in cleavage and formation of ester bonds, while the lipases (EC 3.1.1.3) are hydrolases that catalyze the hydrolysis of triacylglycerols to free fatty acids and glycerol). The use of enzymes in industries allows the development of technological processes as efficient as those carried out by nature and without environmental impacts. In this context, the objective this study was identify microorganisms isolated from Caatinga with promising potential in production of enzymes esterase and lipase. Samples of filamentous fungi (6 samples) Aspergillus sp. genus, called SIS (10, 11, 12, 14, 92, 112) and 2 isolates of Penicillium sp., called SIS (21, 23) were grown in specific culture means using temperatures of 28 °C and 35 °C during 72 h, with daily monitoring. The presence of the characteristic halo showed the presence of the tested enzymes. The best results obtained indicated the production of lipase and esterase by Penicillium sp. (SIS 23) with enzyme index of 2.0 cm at 35 °C and at 28 ° C and 1.5 cm after 72 hours cultivation standing out as potential producer of esterase and lipase.

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“…It is known that various fats, fatty acids, plant oils, triglycerides, ester-based detergents, and other substances are the best inducers of lipase synthesis by microorganisms and sources of carbon (35) . From earlier reported result Aspergillus species were used oils from agro-industrial waste (grape seed oil (GSO) and cotton seed oil (CSO)) to enhance lipase production further higher percentage of fatty acid esters (>80%), namely, soybean, olive, and GSO, promoted the highest lipase production (20) . Present study results supports the earlier report on use of cotton seed oil as carbon source to enhance lipase production by Penicillium melinii (35) and Oospora lactis fungi utilized cotton oil soapstock ( oil richer) as carbon source and produced lipase enzyme (26) .…”

Section: Quantitative Analysis Of Lipase Enzyme Under Submerges Fermementioning

confidence: 99%

“…The production process, oils from agro-industrial waste can come forward as efficient enzymes inductor reducing the production cost. Additionally, this process can reduce environmental problems related to agro-industrial disposal (20) . Screening for new microorganisms and their lipolytic enzyme will open simple routes for synthetic processes and consequently new and faster ways to solve environmental problems.…”

Section: Introductionmentioning

confidence: 99%

Isolation, screening and identification of Lipase producing fungi from cotton seed soapstock

Patel¹,

Shah²

2020

IJST

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Background/Objectives: The present study was focused to exploit the indigenous strains of fungi isolated from cotton seed soapstock for the production of the extracellular lipase through submerged fermentation technique. Methods/ Statistical analysis: Cotton seed soapstock samples used in the study contains gelatinous oil richer chemical constituents. In addition, their enrichment and diluted materials were used for the isolation of lipase producing microorganisms on tributyrin agar plates. All isolates were lipase positive confirmed by a qualitative plate assay. Quantitative estimation of Lipase production activity was measured spectrophotometrically using pnitrophenyl palmitate (p-NPP) as a substrate. In order to exploit the isolated fungal strain for industrial usage, other cellulase and protease enzymes were tested by plate assay. Morphological and molecular characteristics of selected isolates were studied. Findings: From enriched soapstock samples, a total of 49 cultures were isolated among them 19 fungal isolates were screened for lipase, cellulase, and protease activity qualitatively by plate assay. Out of 19, six fungi were selected based on their lipase activity. Highly potent Fusarium solani 7F had the ability to produce 5.95 U/mL/min. crude lipase whereas Aspergillus niger 13F has 4.2 U/mL/min. after 4days incubation at 30oC. Potent fungi culture was identified by morphological, cultural, and molecular characteristics (18s rRNA gene sequence and phylogenetic analysis) revealed them as Penicillium griseofulvum 5F, Aspergillus flavus 6F, Fusarium solani 7F, Aspergillus niger 12F, Aspergillus niger 13F, and Aspergillus terreus 17F. Novelty: Fungi was the first time reported and isolated from cotton seed soapstock materials. In future studies, this enzyme will be used in the degradation of soapstock and also in the production of biosurfactant from soapstock. Keywords: Cotton seed soapstock; Fusarium solani 7F; p-nitrophenyl palmitate; Tributyrin agar plates; degradation

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Biotechnological valorization of oils from agro-industrial wastes to produce lipase using Aspergillus sp. from Amazon

Cited by 33 publications

References 45 publications

Utilization of Clay Materials as Support for Aspergillus japonicus Lipase: An Eco-Friendly Approach

Utilization of Clay Materials as Support for Aspergillus japonicus Lipase: An Eco-Friendly Approach

Detecção De Esterase E Lipase Produzidas Por Fungos Filamentosos Isolados De Solos Da Caatinga / Detection of Esterase and Lipase Produced by Filamentous Fungi Isolated of Caatinga Soil

Isolation, screening and identification of Lipase producing fungi from cotton seed soapstock

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