Biotechnology and Apogamy in Dryopteris affinis spp. affinis: The Influence of Tissue Homogenization, Auxins, Cytokinins, Gibberellic Acid, and Polyamines

Rivera, Alejandro; Conde, Paula; Cañal, Ma Jesús; Fernández, Helena

doi:10.1007/978-3-319-75103-0_7

Cited by 3 publications

(2 citation statements)

References 62 publications

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“…Cytokinins, together with auxins and gibberellins, accelerated apogamy in regenerated gametophytes of the fern Dryopteris affinis subsp. affinis (Rivera et al, 2018). BAP, zeatin riboside and isopentenyladenine did not affect the formation of new nodes and the stem growth of the water fern Marsilea quadrifolia L., whereas they inhibited the development of rhizome and this effect was proportional to the concentration and duration of treatment (Rolli et al, 2015).…”

Section: Methodsmentioning

confidence: 77%

Endogenous cytokinins dynamics during development of sporophytes of perennial ferns Dryopteris filix-mas and Polystichum aculeatum (Dryopteridaceae)

Веденічева¹,

Kosakivska²

2018

Ukr. Bot. J.

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The qualitative composition and dynamics of cytokinins in the fronds and rhizomes of perennial ferns Dryopteris filix-mas and Polystichum aculeatum have been investigated using high-performance liquid chromatography in combination with mass spectrometry. Sporophytes were studied at the stages of intensive vegetative growth (April), formation of sporangia (May) and sporulation (June). Plants of P. aculeatum were also analyzed at the stage of winter vegetation (February). The accumulation of trans-zeatin in fronds of P. aculeatum was revealed at the intensive growth stage, whereas in D. filixmas, the increase in this cytokinin content was observed during the formation of sporangia. The level of zeatin riboside increased in fronds and rhizomes of both fern species at the stage of sporulation. The cessation of the ferns intensive growth involved the accumulation of conjugate-zeatin-O-glucoside. At certain stages of sporophyte development, isopentenyl-type cytokinins were detected: in D. filix-mas, low levels of isopentenyladenine-during sporulation, and in P. aculeatum, quite significant amounts of isopentenyladenosine and isopentenyladenine-in fronds at the stage of intensive growth and in rhizomes-during sporangia formation. In P. aculeatum fronds, active free cytokinins-zeatin and zeatin riboside-were accumulated during the winter vegetation indicating that they were involved in the maintenance of the plant photosynthetic activity under unfavorable conditions. The root system of both fern species was characterized by a lower level of cytokinins as compared to the aerial part. The dynamics of the spectrum and content of cytokinins in the fern organs was species-specific and indirectly testified to the involvement of these phytohormones in growth and development control. The features of differences and similarities of the regulatory role of cytokinins in ferns and seed plants are discussed.

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Section: Methodsmentioning

confidence: 77%

Endogenous cytokinins dynamics during development of sporophytes of perennial ferns Dryopteris filix-mas and Polystichum aculeatum (Dryopteridaceae)

Веденічева¹,

Kosakivska²

2018

Ukr. Bot. J.

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“…Gametophyte morphogenesis is closely associated with cell division and development and is characterized by differential endogenous phytohormone content. The development of gametophytes and sporophytes is regulated by various hormone systems [ 29 ]. The ABA content of GL was less than half of that of the control and GS, in which gametophytes were relatively immature (GL).…”

Section: Discussionmentioning

confidence: 99%

Culture types and period impact gametophyte morphogenesis and sporophyte formation of eastern bracken

et al. 2021

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Background Liquid suspension culture efficiently proliferates plant cells and can be applied to ferns because it rapidly increases the fresh weight of gametophytes. This study assessed gametophyte proliferation and sporophyte production of Pteridium aquilinum var. latiusculum using a suspension culture method. Results The growth curve linear phase of gametophyte cells was confirmed between 9 and 18 days of culture, and the subculture cycle was determined to be 2 weeks. A double-strength MS medium (fresh weight, 18.0 g) containing 2% sucrose and NH4+:NO3− (120 mM, 40:80) was found to be the optimal liquid medium. Gametophytes obtained after suspension culture for 18 days did not normally form sporophytes in an ex vitro soil environment. However, this issue was resolved after changing the culture type or extending the culture period to 6 weeks. A short suspension culture period increased the fresh weight of fragmented and homogenized gametophytes but yielded numerous relatively immature gametophytes (globular forms of branching gametophytes, BG). Furthermore, differences in gametophyte morphogenesis and development were indicated by changes in endogenous phytohormone content. BG with immature development exhibited high accumulation of zeatin, jasmonic acid, and salicylic acid, and relatively low levels of abscisic acid and indole-3-acetic acid. The immature development of gametophytes directly affected sporophyte formation. Conclusions This study maximized the advantages of liquid suspension culture using eastern bracken gametophytes and provides data to resolve any associated issues, thus facilitating efficient bracken production.

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The Effect of Phytohormones and Inhibitors on Apogamous Gametophytes of Dryopteris affinis ssp. affinis

Granados¹,

Rivera²,

Cañal³

et al. 2022

Ferns

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Biotechnology and Apogamy in Dryopteris affinis spp. affinis: The Influence of Tissue Homogenization, Auxins, Cytokinins, Gibberellic Acid, and Polyamines

Cited by 3 publications

References 62 publications

Endogenous cytokinins dynamics during development of sporophytes of perennial ferns Dryopteris filix-mas and Polystichum aculeatum (Dryopteridaceae)

Endogenous cytokinins dynamics during development of sporophytes of perennial ferns Dryopteris filix-mas and Polystichum aculeatum (Dryopteridaceae)

Culture types and period impact gametophyte morphogenesis and sporophyte formation of eastern bracken

The Effect of Phytohormones and Inhibitors on Apogamous Gametophytes of Dryopteris affinis ssp. affinis

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