Biotechnology and Plant Disease Control-Role of RNA Interference

Wani, Shabir Hussain; Sanghera, Gulzar S.; Singh, Naorem Brajendra

doi:10.4236/ajps.2010.12008

Cited by 37 publications

(13 citation statements)

References 135 publications

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“…(vii) Application of RNA interference RNA interference (RNAi) has emerged as a powerful tool for battling some of the most notoriously challenging diseases caused by viruses, bacteria and fungi (Wani, Sanghera, & Singh, 2013). The application of tissue specific inducible gene silencing in combination with the use of appropriate promoters to silence several genes simultaneously will result in protection of crops against destructive pathogens.…”

Section: (V) Transgenicsmentioning

confidence: 99%

Untitled

2014

Plant Sci Today

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Blast disease caused by fungal pathogen Magnaporthe oryzae is the most severe disease of rice (Oryza sativa L). On an estimate it annually destroys rice, which can feed around 60 million people. Keeping in view the importance of the disease, various management strategies like controlled use of nitrogen fertilizers, application of silica and flooding of paddy fields are the practices in use to reduce the rice blast since long time. Improved chemical methods include utilization of copper fungicides, organomercuric and organophosphorus compounds. Some antibiotics e.g., Blasticidin S and Kasugamycin and many systemic and site specific fungicides including melanin biosynthesis inhibitors and plant activators were also utilized effectively for blast management. In the recent years leaf extracts of tulsi and bael have been found effective. Due to the highly variable nature of M. oryzae, exploitation of durable host resistance has remained a challenging job for plant pathologists and breeders. Lots of efforts have been made worldwide to study the variability in the pathogen and to find out the resistance sources. To date approximately 100 R genes for blast resistance have been mapped and 20 of these genes have been cloned in rice. Now, scientists are looking forward to develop durable resistant varieties through pyramiding of quantitative trait loci and major genes. Among the biocontrol agents, different strains of Bacillus spp. and Streptomyces sindeneusis are in use. The availability of rice and M. oryzae genome sequence data are facilitating blast resistance management program to new paradigms which includes isolation and characterization of R and Avr genes, development of noble fungicides, transformed bioagents, transgenic rice and durable resistance.

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Section: (V) Transgenicsmentioning

confidence: 99%

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2014

Plant Sci Today

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“…Wani et al. (2010) reviwed the role of RNA interference (RNAi) in the development of disease‐resistant varieties and further suggested that such goal can be achieved in a more selective and robust manner with the success of genetic engineering techniques.…”

Section: Control Measuresmentioning

confidence: 99%

Biology, Epidemiology and Management of the Pathogenic Fungus Macrophomina phaseolina (Tassi) Goid with Special Reference to Charcoal Rot of Soybean (Glycine max (L.) Merrill)

Gupta¹,

Sharma²,

Ramteke³

2012

Journal of Phytopathology

166

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The fungus Macrophomina phaseolina is a causative agent of diseases in more than 500 plant species. The fungus is primarily soil‐inhabiting but is also seed‐borne in many crops including soybean. It survives in the soil mainly as microsclerotia that germinate repeatedly during the crop‐growing season. Low C : N ratio in the soil and high bulk density as well as high soil moisture content adversely affect the survival of sclerotia. The disease can be managed to some extent by cultural practices, organic amendments, seed treatment and genetic host resistance. The scattered literature on these aspects is reviewed in this paper.

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“…In this method, either RNA/ DNA viruses have been modified as vectors to silence endogenous plant genes. For the process, cloning homologous gene fragments into the virus without compromising viral replication and movement is aberrant / transgene coded RNAs required (Wani et al, 2010) .The viral replication in plants generates dsRNA molecules that triggers PTGS response in plants. This results in reduction of virus titres in local and distant leaves and plant recovery phenotype.…”

Section: Introductionmentioning

confidence: 99%

“…So, that multiple viruses can simultaneously be tangled and achieved a broader resistance. For this purpose, transgenic plants with multiple hpRNA constructs form multiple viral sources or a single hpRNA construct pyramided with different viral sequence need to be created (Wani et al, 2010). Agro-infiltration: Agro-infiltration is a strategy to induce a transient RNA silencing system to directly deliver the RNA silencing molecules into plant tissues (Duan et al, 2012).…”

Section: Introductionmentioning

confidence: 99%

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RNA interference- a novel approach for plant disease management

Puyam

Sharma

Kashyap

2017

JANS

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RNA interference (RNAi) is an incredible revolution in the field of functional genomics, a breakthrough in plant molecular genetics. This technology will generate enormous potential for engineering control of gene expres-sion. The success of managing biotic stress using RNAi technology will prove to be biologically and environmentally safe. It is therapeutic in approach as the resistance induced by RNAi is triggered by ds RNA that results in silencing of specific genes before being translated in a homology dependent manner. Over the time, RNAi is significantly proving it as one of the most promiscent management strategy which eliminates certain risks associated with the development of transgenic plants. This review gives an insight into the probability of management of plant diseases caused by various biotic agents viz. fungi, bacteria and viruses using RNA interference technique and host-pathogen related targeted sites.

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Biotechnology and Plant Disease Control-Role of RNA Interference

Abstract: Development of crop varieties which are resistant against many economically important diseases is

Cited by 37 publications

References 135 publications

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Biology, Epidemiology and Management of the Pathogenic Fungus Macrophomina phaseolina (Tassi) Goid with Special Reference to Charcoal Rot of Soybean (Glycine max (L.) Merrill)

RNA interference- a novel approach for plant disease management

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