Biotechnology applied to fish reproduction: tools for conservation

Siqueira‐Silva, Diógenes Henrique de; Saito, Taiju; Santos-Silva, Amanda Pereira dos; Costa, Raphael da Silva; Pšenička, Martin; Yasui, George Shigueki

doi:10.1007/s10695-018-0506-0

Cited by 41 publications

(21 citation statements)

References 86 publications

(145 reference statements)

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“…Cryopreservation is a powerful method for preserving precious genetic resources as this technology requires no special and expensive equipment and is feasible as long as liquid nitrogen and cryo-containers are available. Because a cryopreservation technique for fish eggs is not yet available, a combination of cryopreservation and transplantation of germline stem cells could be extremely important for preserving the genetic resources of endangered species [details of these techniques can be found in other review articles (Yoshizaki et al 2011;Yoshizaki and Lee 2018;de Siqueira-Silva et al 2018)].…”

Section: Cryopreservation Of Germline Stem Cells From a Superior Breedmentioning

confidence: 99%

Application of surrogate broodstock technology in aquaculture

Yoshizaki

Yazawa

2019

Fish Sci

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Surrogate broodstock technology facilitates the production of donor-derived gametes in surrogates, and comprises transplanting germ cells of a donor into recipients of a different strain or different species. The following applications of this technology are expected in the field of aquaculture: (1) the efficient and reliable production of offspring carrying superior genetic traits by transplanting donor germ cells from a single selected fish with superior traits into many recipient fish; (2) the reduction of the time required to breed fish by using a recipient species with a short generation time to produce gametes of a species with a long generation time; (3) the long-term storage of valuable species or strains as genetic resources by cryopreserving germ cells for transplantation; (4) the mass production of genetically sterile fish by transplanting germ cells of a donor fish that is sterile due to a mutation in the somatic cells into normal recipients without this mutation. It is expected that a combination of these techniques will greatly accelerate the breeding of aquaculture species. It is important to adapt surrogate broodstock technology to a wider range of fishery species and further improve the efficiency of donor-derived gamete production when using surrogate broodstock.

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Section: Cryopreservation Of Germline Stem Cells From a Superior Breedmentioning

confidence: 99%

Application of surrogate broodstock technology in aquaculture

Yoshizaki

Yazawa

2019

Fish Sci

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“…Nowadays, wide pallet of GSCs transplantation methods as well sterilization methods became available and veri ed on several sh species [11,41]. Our strategy is based on complete depletion of endogenous PGCs via dead end gene knockdown as this gene is essential for PGCs migration and maintenance and its suitability for sterilization has been documented in several sh species [42].…”

Section: Discussionmentioning

confidence: 99%

Production of common carp donor-derived offspring from goldfish surrogate broodstock

Franěk

Kašpar

Gela

et al. 2020

Preprint

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Background: Common carp is the fourth most-produced species in worldwide aquaculture. Significant efforts are invested in breeding and preservation of genetic integrity of this important species. However, maintaining carp gene bank in situ can be considered as demanding due to its big body size. Recent progress in reproductive biotechnologies in fish allows improving some unfavourable characteristics of a target species using surrogate reproduction. Germ stem cells (gamete precursors) from one species are transplanted into different surrogate species with small body size. After maturation, surrogates are producing donor-derived progeny. Efficient protocols for cryopreservation of carp male and female germ stem cells have been developed lately. Thus, the next logical goal was to assess the potential of goldfish surrogate to produce donor-derived gametes of common carp after intraperitoneal transplantation of testicular cells. Results: High transplantation success was achieved when 44% of the surviving goldfish produced pure donor-derived gametes of common carp. More importantly, both viable eggs and sperm giving rise to pure common carp progeny were produced, witnessing sustainability of the presented method. Donor-derived identity of the offspring was confirmed by genotyping and typical phenotype corresponding to the donor species. Reproductive performance of chimeras was similar to goldfish controls. Assessment of gamete characteristics showed that the size of donor-derived eggs is between control carp and goldfish eggs. Interestingly, flagellum length in donor-derived spermatozoa was comparable to common carp flagellum and significantly shorter than goldfish flagellum. Conclusions: In this study, we succeeded in the production of pure common carp progeny from surrogate goldfish recipients transplanted intraperitoneally by testicular germ cells. Here we reported production of viable eggs between most distant species up to date. Good reproductive performance of goldfish germline chimeras gives a promising prospect for further analysis about the long-term reproductive performance of surrogates, recovery of cryopreserved germ cells or production of monosex stocks. Presented technology is ready to ease needs for carp breeds preservation and their recovery using many times smaller goldfish surrogates.

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“…Reproduction using wild-specimens, followed by the rearing of juveniles, may work synergically for GenBank since those juveniles could be easily domesticated and used for reproductive technologies such as surrogate propagation (De Siqueira-Silva et al, 2018).…”

Section: Discussionmentioning

confidence: 99%

Capturing, induced spawning, and first feeding of wild-caught Pseudopimelodus mangurus, an endangered catfish species

Arashiro

Yasui

Calado³

et al. 2020

Lat. Am. J. Aquat. Res.

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This study aimed to describe a procedure for sampling, reproduction, and first feeding of the Neotropical catfish Pseudopimelodus mangurus, an endangered fish species. Wild adult P. mangurus specimens were collected in the Mogi Guassu River and subsequently induced to spawn in laboratory conditions. After hand-stripping, the females, the average weight of the oocytes was 143 ± 1.6 g, with a fecundity of 718 ± 49.8 oocytes g-1. The diameter of the oocytes non-hydrated was 1,226.3 ± 47.7 μm to 1,761.2 ± 26.4 μm after hydration. The fertilization rates were 98.00 ± 0.63%, and the hatching rate was 68.94 ± 11.83%. The first feeding was made three days post-hatching with six different treatments, in which the best results arose with sequential feeding with Artemia nauplii, Astyanax altiparanae and Prochilodus lineatus larvae. This condition resulted in a final length of 2,012.7 ± 44.8 μm by the 10th day of the experiment, higher survival (65 ± 0.9%) and lower cannibalism rate (14 ± 0.3%). The data obtained in this study is important for the establishment of reproductive biotechniques, mass production of P. mangurus, and future establishment of ex-situ GenBank.

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Biotechnology applied to fish reproduction: tools for conservation

Cited by 41 publications

References 86 publications

Application of surrogate broodstock technology in aquaculture

Application of surrogate broodstock technology in aquaculture

Production of common carp donor-derived offspring from goldfish surrogate broodstock

Capturing, induced spawning, and first feeding of wild-caught Pseudopimelodus mangurus, an endangered catfish species

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