Biotemplated Synthesis of Gold Nanoparticle–Bacteria Cellulose Nanofiber Nanocomposites and Their Application in Biosensing

Abstract

Bacteria cellulose (BC) nanofibers are used as robust biotemplates for the facile fabrication of novel gold nanoparticle (NP)–bacteria cellulose nanofiber (Au–BC) nanocomposites via a one‐step method. The BC nanofibers are uniformly coated with Au NPs in aqueous suspension using poly(ethyleneimine) (PEI) as the reducing and linking agent. With the addition of different halides, Au–BC nanocomposites with different Au shell thicknesses are formed, and a possible formation mechanism is proposed by taking into account the special role played by PEI. A novel H2O2 biosensor is constructed using the obtained Au–BC nanocomposites as excellent support for horseradish peroxidase (HRP) immobilization, which allows the detection of H2O2 with a detection limit lower than 1 µM. The Au–BC nanocomposites could be further used for the immobilization of many other enzymes, and thus, may find potential applications in bioelectroanalysis and bioelectrocatalysis.

Self-Assembled Peptide Nanofiber Templated One-Dimensional Gold Nanostructures Exhibiting Resistive Switching

“…A similarly broad signal for nanowires composed of nanoparticles arranged on a template was reported previously. 8 The nanofibers are coated with smalldiameter gold nanoparticles, and the electromagnetic properties of such closely separated nanoparticle arrays significantly differ from those of monolithic gold nanorods due to the presence of gaps between the nanoparticles. The Au nanoparticles were observed to have diameters below 15 nm, and it is wellestablished both theoretically and experimentally that Au nanoparticles and rods begin to exhibit SPR peaks when the characteristic size exceeds ca.…”

“…7 In another study, bacteria cellulose fibers were used for one-step preparation of gold-bacteria cellulose nanocomposites where gold was reduced and linked using poly(ethyleneimine). 8 Amyloid-like peptide (ALP) nanostructures also provide quite versatile opportunities for controlled aggregation of metal nanoparticles into one-dimensional nanostructures which are of interest due to their exciting physical, mechanical, and chemical properties. 2,9 ALP nanostructures can be specifically designed to have chemically active groups offering metal binding sides on their external surface.…”

Nanostructured Assemblies of Gold and Silver Nanoparticles for Plasmon Enhanced Spectroscopy Using Living Biotemplates

“…The focus on the use of wet chemistry to obtain nanostructured materials is an attempt to explore the stability of colloidal nanoparticles to study hybrid materials. There are several examples regarding the use of living templates for noble metal nanoparticles, such as fungus [22,23], yeast [24], viruses [18], or supramolecular structures of biological origin [25].…”

Bio-Inspired Synthesis of Electrode Materials for Lithium Rechargeable Batteries

“…When the adsorbed precursors react exclusively on the surface to form the target materials, the complex shapes of the biomaterials are duplicated generating isostructural target materials. Some examples of duplicated nanomaterials are presented in Figure 3 (Zhou et al, 2007;Zhang et al, 2010). Nanomaterials can fully or partially cover the surface of the biomaterials, forming nanoarchitecture similar to that of the template.…”

“…theromophilus (left) and ZnO hollow nanospheres fabricated using Str. theromophilus as the structural template (right) (Zhou et al, 2007) and (b) bacteria-cellulose nanofibers (left) and Au-bacterial-cellulose nanocomposite (right) (Zhang et al, 2010).…”