Biotic elicitor enhanced production of psoralen in suspension cultures of Psoralea corylifolia L.

Abstract

Cell cultures of Psoralea corylifolia L. were established from the leaf disk derived callus. The effect of different biotic elicitors prepared from the fungal extract (Aspergillus niger and Penicillium notatum), yeast extract and chitosan with different concentrations was studied. The increased synthesis of psoralen in 16-day old cell cultures under 16 h of light and 8 h of dark period was studied. Elicitation of psoralen in A. niger elicitor treated cells was found 9-fold higher over control cells. Treating the cells with P. notatum, yeast extract and chitosan elicitors lead to four to seven-fold higher psoralen accumulation over control cells. The extract of A. niger at 1.0% v/v increased the significant accumulation of psoralen (9850 μg/g DCW) in the cultured cells. Our study clearly shows that all the elicitors had the potential to increase the accumulation of psoralen but the A. niger elicitor at 1.0% v/v induced maximum accumulation.

Impact of Biotic and Abiotic Elicitation on Morphology, Growth, Active Constituents and Antibacterial Activity of Solanum Nigrum (L.) Calli Induced in Vitro

“…The results of the present study may agree with Dixon and Priva (1995), who mentioned that soluble phenolics, which represent the most widely distributed secondary metabolites in the plant kingdom, could be enhanced as a powerful antioxidant in plant tissues suffering from different stress factors. The results obtained in this study that biotic elicitation may enhance the biosynthesis of secondary metabolites in callus cultures may agree with previous observations, which were made on other plants by Baldi et al (2009), Karwasara et al (2011), Mendhulkar and Vakil (2013, Swaroopa et al (2013), Tahsili et al (2013), Ahmed and Baig (2014), Ebrahimi and Zarinpanjish (2015) and Gadzovska Simic et al (2015). Elicitation is done to enhance the production of secondary metabolites in plant cultures by using biotic/abiotic molecules.…”

Establishment of suspension cultures of Salvadora persica L. for benzyl isothiocyanate production

“…The increase in BITC accumulation with elicitor application was observed and the increase in elicitor concentration over the optimum concentration led to decrease in the BITC content in callus cultures. Similar results were reported in taxol [38] , ajmalicine [39] and psoralen [4] production.…”

“…Fig. 2 shows the effect of elicitation with the optimum concentration of chitosan (100 mg/L) on [41] and Ahmed and Baig [4] , who stated that the amount of metabolite production varied with duration time of incubation with elicitors. The optimum incubation time for BITC accumulation with chitosan was found to be 21 days for both shake-flask culture and bioreactor.…”

“…Among other advantages, plant cell and tissue cultures lead to have a higher rate of metabolism when compared to field grown plants [2,3] . Furthermore, in vitrocultures offer uniform secondary product synthesis under controlled conditions by overcoming the effect of climatic conditions and diseases in field grown or natural plants [4] . Various strategies have been developed to improve the in vitro production of secondary metabolites [5] .…”

Elicitation induced flavonoids, phenolic constituents, antioxidant and cytotoxic activities of Artemisia monosperma callus cultures

“…5, 10 and 15 mg/L (Ahmed and Baig, 2014) and calcium chloride obtained from ElNasr Pharmaceutical Chemicals Company (0.2, 0.4 and 0.6 mg/L) were added as dried powder separately to M&S1 media before autoclaving (Baldi and Dixit, 2008). Fusarium oxysporum hyphae, supplied from Faculty of Agriculture, Zagazig University was washed with distilled water, filtered, dried at 40°C till constant weight and finely powdered before addition to M&S1 media at 1, 2 and 3 mg/L (Patel and Krishnamurthy, 2013).…”