Biotic stress inducible promoters in crop plants-a review

Muthusamy, SK; Pn, Sivalingam; J, Sridhar; Singh, D. B.; SM, Haldhar; Kaushal, Pankaj

doi:10.53911/jae.2017.4202

Cited by 20 publications

(5 citation statements)

References 29 publications

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“…To validate its functions, we cloned the promoter of the AhOMT1 gene and used it to drive the GUS gene under A. flavus infection in transgenic plants. The online promoter analysis tools predicted several important cis -regulatory elements, including TATA Box, CAAT Box, GC Box ( Muthusamy et al., 2017 ), and many others related to hormones, light, growth, regulation, and stress responsiveness. Different kinds of elements and their number critically determine the strength of a promoter ( Stålberg et al., 1993 ; Tang et al., 2015 ).…”

Section: Discussionmentioning

confidence: 99%

Molecular cloning and functional characterization of the promoter of a novel Aspergillus flavus inducible gene (AhOMT1) from peanut

Zhuang

Sharif²,

Zeng³

et al. 2023

Front. Plant Sci.

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Peanut is an important oil and food legume crop grown in more than one hundred countries, but the yield and quality are often impaired by different pathogens and diseases, especially aflatoxins jeopardizing human health and causing global concerns. For better management of aflatoxin contamination, we report the cloning and characterization of a novel A. flavus inducible promoter of the O-methyltransferase gene (AhOMT1) from peanut. The AhOMT1 gene was identified as the highest inducible gene by A. flavus infection through genome-wide microarray analysis and verified by qRT-PCR analysis. AhOMT1 gene was studied in detail, and its promoter, fussed with the GUS gene, was introduced into Arabidopsis to generate homozygous transgenic lines. Expression of GUS gene was studied in transgenic plants under the infection of A. flavus. The analysis of AhOMT1 gene characterized by in silico assay, RNAseq, and qRT-PCR revealed minute expression in different organs and tissues with trace or no response to low temperature, drought, hormones, Ca2+, and bacterial stresses, but highly induced by A. flavus infection. It contains four exons encoding 297 aa predicted to transfer the methyl group of S-adenosyl-L-methionine (SAM). The promoter contains different cis-elements responsible for its expression characteristics. Functional characterization of AhOMT1P in transgenic Arabidopsis plants demonstrated highly inducible behavior only under A. flavus infection. The transgenic plants did not show GUS expression in any tissue(s) without inoculation of A. flavus spores. However, GUS activity increased significantly after inoculation of A. flavus and maintained a high level of expression after 48 hours of infection. These results provided a novel way for future management of peanut aflatoxins contamination through driving resistance genes in A. flavus inducible manner.

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Section: Discussionmentioning

confidence: 99%

Molecular cloning and functional characterization of the promoter of a novel Aspergillus flavus inducible gene (AhOMT1) from peanut

Zhuang

Sharif²,

Zeng³

et al. 2023

Front. Plant Sci.

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“…At the same time, Xinhuixiaoli cultivar (disease-resistant; fungal and bacterial diseases), was used for cloning of promoter based on its possible future use to develop transgenic peanut for disease resistance. Online databases for promoter analysis predicted many cis -acting elements, including core promoter element as TATA Box and proximal control elements as CAAT Box, GC Box ( Muthusamy et al, 2017 ), and many other light-responsive, hormones responsive, growth and regulation responsive, and stress-responsive elements. The number and types of cis -regulatory elements are important determinants of promoter strength and specificity ( Stålberg et al, 1993 ; Tang et al, 2015 ).…”

Section: Discussionmentioning

confidence: 99%

Cloning and Functional Characterization of a Pericarp Abundant Expression Promoter (AhGLP17-1P) From Peanut (Arachis hypogaea L.)

et al. 2022

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Peanut (Arachis hypogaea L.) is an important oil and food legume crop grown in tropical and subtropical areas of the world. As a geocarpic crop, it is affected by many soil-borne diseases and pathogens. The pericarp, an inedible part of the seed, acts as the first layer of defense against biotic and abiotic stresses. Pericarp promoters could drive the defense-related genes specific expression in pericarp for the defense application. Here, we identified a pericarp-abundant promoter (AhGLP17-1P) through microarray and transcriptome analysis. Besides the core promoter elements, several other important cis-elements were identified using online promoter analysis tools. Semiquantitative and qRT-PCR analyses validated that the AhGLP17-1 gene was specifically expressed only in the pericarp, and no expression was detected in leaves, stem, roots, flowers, gynophore/peg, testa, and embryo in peanut. Transgenic Arabidopsis plants showed strong GUS expression in siliques, while GUS staining was almost absent in remaining tissues, including roots, seedlings, leaf, stem, flowers, cotyledons, embryo, and seed coat confirmed its peanut expressions. Quantitative expression of the GUS gene also supported the GUS staining results. The results strongly suggest that this promoter can drive foreign genes’ expression in a pericarp-abundant manner. This is the first study on the functional characterization of the pericarp-abundant promoters in peanut. The results could provide practical significance to improve the resistance of peanut, and other crops for seed protection uses.

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“…All the above mentioned expression control systems open up uncountable combinatorial possibilities for the genetic circuits designed for plants. One can also oversee that agricultural applications are under interest to activate synthetic genetic networks with identified promoters responsive to biotic and abiotic stress (Singhal et al, 2016;Muthusamy et al, 2017) or synthesized to switch on with fertilization (Crawford et al, 2010).…”

Section: Circuit Design and Controlmentioning

confidence: 99%

Biocircuits in plants and eukaryotic algae

2022

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As one of synthetic biology’s foundations, biocircuits are a strategy of genetic parts assembling to recognize a signal and to produce a desirable output to interfere with a biological function. In this review, we revisited the progress in the biocircuits technology basis and its mandatory elements, such as the characterization and assembly of functional parts. Furthermore, for a successful implementation, the transcriptional control systems are a relevant point, and the computational tools help to predict the best combinations among the biological parts planned to be used to achieve the desirable phenotype. However, many challenges are involved in delivering and stabilizing the synthetic structures. Some research experiences, such as the golden crops, biosensors, and artificial photosynthetic structures, can indicate the positive and limiting aspects of the practice. Finally, we envision that the modulatory structural feature and the possibility of finer gene regulation through biocircuits can contribute to the complex design of synthetic chromosomes aiming to develop plants and algae with new or improved functions.

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Biotic stress inducible promoters in crop plants-a review

Cited by 20 publications

References 29 publications

Molecular cloning and functional characterization of the promoter of a novel Aspergillus flavus inducible gene (AhOMT1) from peanut

Molecular cloning and functional characterization of the promoter of a novel Aspergillus flavus inducible gene (AhOMT1) from peanut

Cloning and Functional Characterization of a Pericarp Abundant Expression Promoter (AhGLP17-1P) From Peanut (Arachis hypogaea L.)

Biocircuits in plants and eukaryotic algae

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