Biotin–avidin mediated competitive enzyme-linked immunosorbent assay to detect residues of tetracyclines in milk

Jeon, Moonsun; Kim, Jisun; Paeng, Ki‐Jung; Park, Sung Woo; Paeng, Insook Rhee

doi:10.1016/j.microc.2007.09.001

Cited by 92 publications

(49 citation statements)

References 18 publications

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“…The largest degree of non-specific binding was obtained when using 5% skimmed milk as the blocking reagent. This is probably because of the skimmed milk containing small amounts of endogenous biotin residues which caused a high background signal when there was a biotin-avidin system in the assay [27]. In contrast, the mix of 5% casein and 1% non-protein blocking reagent eliminated virtually all non-specific background binding, thus this mixture served as the blocking agent in the following experiments.…”

Section: Optimization Of the Reaction Conditionsmentioning

confidence: 99%

Detection of immunoglobulin E using an aptamer based dot-blot assay

Wang

2013

Chin. Sci. Bull.

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A novel aptamer based dot-blot assay for the detection of immunoglobulin E (IgE) was developed. A biotinylated aptamer was employed as the bio-recognition element to specifically interact with the target protein immobilized onto a nitrocellulose membrane substrate. Avidin conjugated horseradish peroxidase was introduced onto the membrane through the biotin-avidin system to catalyze the hydrogen peroxide mediated oxidation of 3,3′,5,5′-tetramethylbenzidine, thereby producing the blue-colored insoluble product. The intensity of the dots increased as the concentration of IgE increased. The spot intensity was quantified using a simple portable instrument. A linear response relationship between the spot intensity and the concentration of IgE over the range of 50 nmol/L to 1 μmol/L was obtained. The detection limit for IgE using the aptamer-based assay was 2.89 nmol/L. This assay was found to discriminate IgE from non-target proteins such as thrombin, bovine serum albumin and immunoglobulin G. immunoglobulin E, dot-blot assay, aptamer, biotin-avidin system Citation:Wang Y X, Ye Z Z, Ying Y B. Detection of immunoglobulin E using an aptamer based dot-blot assay.

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Section: Optimization Of the Reaction Conditionsmentioning

confidence: 99%

Detection of immunoglobulin E using an aptamer based dot-blot assay

Wang

2013

Chin. Sci. Bull.

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“…In recent years, new rapid electrochemical methods for TET detection appeared frequently, such as immunosensors [21], enzyme sensors [22] and aptasensor [23]. Aptamers are nucleic acid ligands selected from random sequence pools in vitro, which can be naturally folded into different three-dimensional structures and have the capability of binding specifically to biosurfaces [24][25][26][27].…”

Section: Introductionmentioning

confidence: 99%

Electrochemical Aptasensor Based on Prussian Blue-Chitosan-Glutaraldehyde for the Sensitive Determination of Tetracycline

et al. 2014

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Abstract:In this paper, a novel and sensitive electrochemical aptasensor for detecting tetracycline (TET) with prussian blue (PB) as the label-free signal was fabricated. A PB-chitosan-glutaraldehyde (PB-CS-GA) system acting as the signal indicator was developed to improve the sensitivity of the electrochemical aptasensor. Firstly, the PB-CS-GA was fixed onto the glass carbon electrode surface. Then, colloidal gold nanoparticles (AuNPs) were droped onto the electrode to immobilize the anti-TET aptamer for preparation of the aptasensor. The stepwise assembly process of the aptasensor was characterized by cyclic voltammetry (C-V) and scanning electron microscope (SEM). The target TET captured onto the electrode induced the current response of the electrode due to the non-conducting biomoleculars. Under the optimum operating conditions, the response of differential pulse voltammetry (DPV) was used for detecting the concentration of TET. The proposed aptasensor showed a high sensitivity and a wide linear range of 10 −9 ∼ 10 −5 M and 10 −5 ∼ 10 −2 M with the correlation coefficients of 0.994 and 0.992, respectively. The detection limit was 3.2×10 −10 M (RSD 4.12%). Due to its rapidity, sensitivity and low cost, the proposed aptasensor could be used as a pre-scanning method in TET determination for the analysis of livestock products.

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“…However, literature data showed that recognition of these substances by antibodies with equal or near efficacy proved to be insufficient. Thus, several works have been noted CTC revealed 10 and 14% of TC activity (Jeon, Kim, Paeng, Park, & Paeng, 2008;Pastor-Navarro, Moraisa, Maquieira, & Puchades, 2007), the same indexes for OTC varied between 0 and 30% and cross-reactivity of DC was not more than 5% level (Faraj & Ali, 1981;Jeon et al, 2008;Kumar, Thompson, *Corresponding author. Email: burma68@yandex.ru Singh, Chander, & Gupta, 2004;Pastor-Navarro et al, 2007;Zhang, Lu, Liu, Zhao, & Xi, 2007;R-Biofarm AG).…”

Section: Introductionmentioning

confidence: 99%

Improved group determination of tetracycline antibiotics in competitive enzyme-linked immunosorbent assay

Burkin

Galvidis

2009

Food and Agricultural Immunology

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Seven tetracycline (TC) antibiotics were used as haptens for synthesis of conjugated antigens and as standards in indirect competitive enzyme-linked immunosorbent assay (ELISA). Seven conjugates were prepared by formaldehyde condensation (f) and another seven antigens were synthesised by linking TCs with periodate-oxidised transferrin (TF(pi)). Two gelatineÁlymecycline (LC) conjugates were the products of glutaraldehyde and activated esters reactions. To estimate the influence of solid-phase antigen on assay specificity, all the conjugates were absorbed on polystyrene plates and using anti-bovine serum albuminÁTC(f) sera, the ELISAs were developed and compared. The variant with immobilised TFÁ chlortetracycline (CTC(pi)) showed the best group specificity: recognition of seven TCs differed only in 5.3 times. This assay displayed sensitivity 0.1 ng/ml and crossreactivity indexes: TC (100%), CTC (105%), oxytetracycline (19.8%), methacycline (23.6%), minocycline (70%), doxycycline (25%) and LC (50%). The dependence of ELISA specificity on immobilised antigen spatial structure was demonstrated. Solid-phase conjugate selection is a useful tool for modifying assay specificity.

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Biotin–avidin mediated competitive enzyme-linked immunosorbent assay to detect residues of tetracyclines in milk

Cited by 92 publications

References 18 publications

Detection of immunoglobulin E using an aptamer based dot-blot assay

Detection of immunoglobulin E using an aptamer based dot-blot assay

Electrochemical Aptasensor Based on Prussian Blue-Chitosan-Glutaraldehyde for the Sensitive Determination of Tetracycline

Improved group determination of tetracycline antibiotics in competitive enzyme-linked immunosorbent assay

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