Biotin deficiency stimulates survival pathways in human lymphoma cells exposed to antineoplastic drugs

Griffin, Jacob B.; Zempleni, Janos

doi:10.1016/j.jnutbio.2004.10.004

Cited by 6 publications

(4 citation statements)

References 38 publications

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“…Fisher's Protected Least Significant Difference procedure was used for posthoc testing (31). For pairwise comparisons the paired t test was used to determine significance of differences (32). Gender effects were not tested.…”

Section: Statisticsmentioning

confidence: 99%

Feeding Drosophila a Biotin-Deficient Diet for Multiple Generations Increases Stress Resistance and Lifespan and Alters Gene Expression and Histone Biotinylation Patterns3

Smith

Hoi

Eissenberg

et al. 2007

The Journal of Nutrition

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Energy restriction increases stress resistance and lifespan in Drosophila melanogaster and other species. The roles of individual nutrients in stress resistance and longevity are largely unknown. The vitamin biotin is a potential candidate for mediating these effects, given its known roles in stress signaling and gene regulation by epigenetic mechanisms, i.e. biotinylation of histones. Here, we tested the hypothesis that prolonged culture of Drosophila on biotin-deficient (BD) medium increases stress resistance and lifespan. Flies were fed a BD diet for multiple generations; controls were fed a biotin-normal diet. In some experiments, a third group of flies was fed a BD diet for 12 generations and then switched to control diets for 2 generations to eliminate potential effects of short-term biotin deficiency. Flies fed a BD diet exhibited a 30% increase in lifespan. This increase was associated with enhanced resistance to the DNA-damaging agent hydroxyurea and heat stress. Also, fertility increased significantly compared with biotin-normal controls. Biotinylation of histones was barely detectable in biotin-deprived flies, suggesting that epigenetic events might have contributed to effects of biotin deprivation.

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Section: Statisticsmentioning

confidence: 99%

Feeding Drosophila a Biotin-Deficient Diet for Multiple Generations Increases Stress Resistance and Lifespan and Alters Gene Expression and Histone Biotinylation Patterns3

Smith

Hoi

Eissenberg

et al. 2007

The Journal of Nutrition

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“…The products of these genes enhance survival of cancer cells (70,137,138), causing resistance to chemotherapy. Research has shown that biotin deficiency enhances the nuclear translocation of NF-κB in Jurkat cells treated with antineoplastic agents such as taxol, doxorubicin, and vinblastine (60). This is associated with increased expression of Bfl-1/A1 and a decreased rate of death in cells treated with doxorubicin and vinblastine.…”

Section: Nuclear Factor-κbmentioning

confidence: 99%

Uptake, Localization, and Noncarboxylase Roles of Biotin

2005

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Evidence is emerging that biotin participates in processes other than classical carboxylation reactions. Specifically, novel roles for biotin in cell signaling, gene expression, and chromatin structure have been identified in recent years. Human cells accumulate biotin by using both the sodium-dependent multivitamin transporter and monocarboxylate transporter 1. These transporters and other biotin-binding proteins partition biotin to compartments involved in biotin signaling: cytoplasm, mitochondria, and nuclei. The activity of cell signals such as biotinyl-AMP, Sp1 and Sp3, nuclear factor (NF)-kappaB, and receptor tyrosine kinases depends on biotin supply. Consistent with a role for biotin and its catabolites in modulating these cell signals, greater than 2000 biotin-dependent genes have been identified in various human tissues. Many biotin-dependent gene products play roles in signal transduction and localize to the cell nucleus, consistent with a role for biotin in cell signaling. Posttranscriptional events related to ribosomal activity and protein folding may further contribute to effects of biotin on gene expression. Finally, research has shown that biotinidase and holocarboxylase synthetase mediate covalent binding of biotin to histones (DNA-binding proteins), affecting chromatin structure; at least seven biotinylation sites have been identified in human histones. Biotinylation of histones appears to play a role in cell proliferation, gene silencing, and the cellular response to DNA repair. Roles for biotin in cell signaling and chromatin structure are consistent with the notion that biotin has a unique significance in cell biology.

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“…, 2005). Moreover, biotin deficiency stimulates nuclear factor κB (NF‐κB) survival pathways (Griffin and Zempleni, 2005; Rodriguez‐Melendez and Zempleni, 2003).…”

Section: Introductionmentioning

confidence: 99%

Biotin deficiency causes spontaneous cell death and activation of defense signaling

Li¹,

Brader²,

Helenius³

et al. 2012

The Plant Journal

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SUMMARYIn addition to its essential metabolic functions, biotin has been suggested to play a critical role in regulating gene expression. The first committed enzyme in biotin biosynthesis in Arabidopsis, 7-keto-8-aminopelargonic acid synthase, is encoded by At5g04620 (BIO4). We isolated a T-DNA insertion mutant of BIO4 (bio4-1) with a spontaneous cell death phenotype, which was rescued both by exogenous biotin and genetic complementation. The bio4-1 plants exhibited massive accumulation of hydrogen peroxide and constitutive up-regulation of a number of genes that are diagnostic for defense and reactive oxygen species signaling. The cell-death phenotype was independent of salicylic acid and jasmonate signaling. Interestingly, the observed increase in defense gene expression was not accompanied by enhanced resistance to bacterial pathogens, which may be explained by uncoupling of defense gene transcription from accumulation of the corresponding protein.Characterization of biotinylated protein profiles showed a substantial reduction of both chloroplastic biotinylated proteins and a nuclear biotinylated polypeptide in the mutant. Our results suggest that biotin deficiency results in light-dependent spontaneous cell death and modulates defense gene expression. The isolation and molecular characterization of the bio4-1 mutant provides a valuable tool for elucidating new functions of biotin.

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Biotin deficiency stimulates survival pathways in human lymphoma cells exposed to antineoplastic drugs

Cited by 6 publications

References 38 publications

Feeding Drosophila a Biotin-Deficient Diet for Multiple Generations Increases Stress Resistance and Lifespan and Alters Gene Expression and Histone Biotinylation Patterns3

Feeding Drosophila a Biotin-Deficient Diet for Multiple Generations Increases Stress Resistance and Lifespan and Alters Gene Expression and Histone Biotinylation Patterns3

Uptake, Localization, and Noncarboxylase Roles of Biotin

Biotin deficiency causes spontaneous cell death and activation of defense signaling

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