Biotin self-assembled monolayer for impedimetric genosensor for direct detection of HIV-1

Designing of a unique bioreceptor and fabrication of an efficient genosensing platform for neonatal sepsis detection

“…7 For the development of efficient electrochemical biosensors, the selection of the target analyte as well as the nanomaterial plays an important role. Recently, efforts have been made towards the development of DNA-based genosensors for the detection of Neisseria gonorrhoeae , 8 E. coli , 9 group B Streptococcus , 10 Listeria monocytogenes , 11 Haemophilus influenzae , 12 Legionella pneumophila , 13 human immunodeficiency virus (HIV), 14 etc. DNA-based biosensors offer several advantages in terms of specificity (single-base mismatch), ultralow detection limit, and simplicity of instrumentation and they can be easily expanded into a multiplex detection platform compared to antibody, cell and enzyme based biosensors.…”

Proximity sensitive detection of microRNAs using electrochemical impedance spectroscopy biosensors

“…Altering fundamental parameters in hybridisation events by changing probe length, orientation of probe binding on electrode surface, target length and by creating of overhang sequences at both end of the probe can provide significant changes in the EIS signal and this may lead to the improved detection methodology in miRNA sensing. Most of the previous EISbased studies mainly focused on SAM layer organisation (Cui et al 2017;Park et al 2008;Tamayo et al 2020), probe immobilisation methods (Li et al 2021;Peng et al 2007), probe types (Keighley et al 2008a;Keighley et al 2008b;Wang et al 2008), surface probe density (Brothers et al 2020;Keighley et al 2008a;Keighley et al 2008b) and surface materials (Chen et al 2009;Nguy et al 2017) to improve nucleic acid hybridisation based sensor performance.…”