Biotin-streptavidin amplified enzymeimmunoassay for 13, 14-dihydro-15-keto-PGF2α

Meyer, Heinrich; Eisele, Karl; Osaso, J.

doi:10.1016/0090-6980(89)90140-8

Cited by 14 publications

(7 citation statements)

References 9 publications

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“…13,14-dihydro-15-keto PGF2a 13,14-dihydro-15-keto PGF2a (PGFM), the first and major stable metabolite of PGF2a, is a successful marker of in vivo PGF2a production (30)(31)(32). The measurement of PGFM reflects PGF2a because it is rapidly metabolized to its metabolite; therefore, we will base our discussion on studies with PGF2a and PGFM.…”

Section: Thromboxane B2mentioning

confidence: 99%

Comparison of endometrial prostanoid profiles in three infertile subgroups: the missing part of receptivity?

Keleş

Ülgen

Erkan

et al. 2020

Fertility and Sterility

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Objective: To study the prostanoid profile of the endometria of patients with recurrent implantation failure (RIF), unexplained infertility (UIF), and recurrent miscarriages (RM), and to compare them with the endometria of healthy fertile controls. Design: Prospective cohort study. Setting: University hospital. Patient(s): Fifteen patients with RIF, 18 patients with UIF, 16 patients with RM, and 23 fertile controls were recruited. Intervention(s): Endometrial samples were taken during the window of implantation. After tissue homogenization and extraction, analysis with ultra-performance liquid chromatography diode array detector electrospray ionisation tandem mass spectrometry was performed. Main Outcome Measures: Concentrations of prostaglandin (PG) D1,

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Section: Thromboxane B2mentioning

confidence: 99%

Comparison of endometrial prostanoid profiles in three infertile subgroups: the missing part of receptivity?

Keleş

Ülgen

Erkan

et al. 2020

Fertility and Sterility

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“…Homogenate was centrifuged at 570 x g for 10 min and the supernatant was decanted and dried up at 50°C. Extraction of the prostaglandin was carried out by employing liquid chromatography using Baker-spe octadecyl (C,,) silicagel column (NEN Research Products, Du Pont Company, Biotechnology Systems, Wilmington, VT, USA) as described by Meyer et al (1989). Samples were dissolved in l-ml assay buffer and stored at -20°C until further assay.…”

Section: Prostaglandin Fzrmentioning

confidence: 99%

“…The concentrations of PGFk were quantified by enzyme immunoassay which was basically the same method for prostaglandin FM, as described by Meyer et al (1989). The standard curve ranged from 9.8-20 000 PM.…”

Section: Prostaglandin Fzrmentioning

confidence: 99%

Evidence for Uterine Horn Differences in Local Concentrations of Oxytocin, Oxytocin Receptor and Prostaglandin F_2α during the Estrous Cycle and Early Pregnancy in Cows

Cerbito

Miyamoto

Sauerwein

et al. 1997

Reprod Domestic Animals

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ContentsThe current study investigated the endometrial concentration of oxytocin (OT), oxytocin receptors (OT-R) and prostaglandin Fzc, (PGF2?) ipsilateral and contralateral to the corpus luteum (CL) and their inter-relationships during the estrous cycle and early pregnancy. Reproductive tracts of 45 Holstein COWS collected from the slaughterhouse were used. Estimation of the stages of the estrous cycle was determined, based on CL characteristics and progesterone P4 levels. The luteal stage was characterized by higher OT levels in the ipsilateral horn than contralateral horn, while OT-R and PGFzl levels were low and similar in both uterine horns. At follicular stage, thc ipsilateral horn showed significantly higher concentrations of OT, OT-R and PGF2, than the contralateral horn. At post-ovulation, OT levels remained high and significantly higher in the ipsilateral horn. The OT-R and PGF2, levels decreased to a certain level but were not significantly different from their levels at the follicular stage, and no difference was found between horns. At early pregnancy, OT levels were low, OT-R levels were significantly lower in the ipsilateral than in the contralateral horn. and PGF2, decreased, but the levels were higher than at luteal phase. The results indicate clear stage-dependent changes of endocrine parameters within ipdateral and contralateral horn in relation to the CL and suggest that the horn adjacent to CL is more active, sensitive and responsive to the cause and effect of luteolysis, supporting the concept of a utero-ovarian local regulating mechanism. Further, the significantly low OT-R in the ipsilateral horn suggests a local effect of the CL or the embryo. Inhalt Unterschiedliche lokale Konzentrationen von Oxytocin, Oxytocin-Rezeptor und Prostaglandin FZx in den Uterushiirnern bei zyklischen und friihtragenden KuhenIn der vorleigenden Untersuchung wurde die endometrische Konzentration von Oxytocin (OT), Oxytocin-Rezeptor (OT-R) und Prostaglandin F2, (PGF2,) im ipsi-und kontralateralen Horn zum Corpus luteum (CL) untersucht und die Abhlngigkeit wahrend der Brunst und der friihen Trachtigkeit bestimmt. Es wurden Genitalorgane (Schlachtorgane) von 45 HolsteinKiihen venvendet. Die Schatzung des Zyklusstadiums basicrte auf den Merkmalen der Gelbkorper-und der P3 Level. In der Gelbkorperphase waren charakteristisch hohcre OT-Spiegel im ipsilateralen Horn als im kontralateralen Horn zu finden, wohingegen OT-R und PGF2, in beiden Hornern gering waren. In der Follikelphase wies das ipsilaterale Horn im Vergleich zum kontralateralen signifikant hohere Konzentrationen von OT, OT-R und PGF2, auf. Postovulatorisch verblieben die OTWerte auf hoherem Niveau und waren im ipsilateralcn Horn signifikant hoher. OT-R-und PGF2,-Werte sanken bis auf ein bestimmtes Niveau, aber waren nicht signifikant unterschiedlich zu den entsprechenden Werten der Follikelphase. Zwischen den beiden Hornern wurden keine Unterschiede gefunden. Wahrend der friihen Trachtigkeit waren die OTWerte niedrig, die OT-R-Level signifikant geringer im...

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“…PGFM determination: PGFM concentrations were determined by a second antibody EIA as reported previously [9]. The standard curve for PGFM ranged from 4 to 1,000 pg/ml, and the ED 50 of the assay was 48 pg/ml.…”

Section: Animalsmentioning

confidence: 99%

Effective Oxytocin Treatment on Placental Expulsion after Foaling in Heavy Draft Mares

Ishii

Kobayashi

Acosta

et al. 2009

The Journal of Veterinary Medical Science

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ABSTRACT. The aim of this study was to establish the effectiveness of administration of oxytocin (OT) on placental expulsion after foaling. Four foaling mares with the placentas retained for up 1 hr after foaling received OT (50 IU) administration at 1 hr intervals before expulsion of the placenta. The changes in the plasma concentrations of OT and the PGF2alpha metabolite (PGFM) were investigated, and the influence of OT administration was considered. The results were as follows. The placenta was expelled after one to three OT administrations in all four mares that received OT. In two mares, which expelled the placenta within 30 min after OT administration, the OT concentration increased and remained high. Expulsion of the placenta was delayed in two mares, and one of these mares, which received three doses of OT beginning 1 hr after foaling, showed only a small increase in the OT concentration after the first administration; the other mare did not receive OT until 3 hr after foaling. The OT concentration was increased before placental expulsion in all the mares, and the PGFM concentration also increased in the two mares with retained placentas. In conclusion, we suggest that intramuscular administration of 50 IU of OT at 1-hr intervals beginning 1 hr after foaling is effective for inducing placental expulsion. KEY WORDS: mare, oxytocin, PGFM, placental expulsion.J. Vet. Med. Sci. 71(3): 293-297, 2009 In heavy draft mares, retained placenta occurs frequently and can have serious consequences, such as puerperal fever and laminitis. Extension of the placental retention time reduce the subsequent conception rate in heavy draft mares [5]. Although the conception rate of foal heat in mares with a placental retention time of less than 1 hr is 66%, it falls significantly to 51.7% in mares with a placental retention time that exceeds 4 hr [5]. To promote expulsion of the placenta, oxytocin (OT) administration is usually used in mares after foaling. There are some reports that describe effective OT treatments for retained placenta, such as an intravenous drip infusion containing 30-100 IU of OT [3,14] and repeated subcutaneous or intramuscular administration of 20-120 IU of OT [4,7,16]. Ishii et al. [5] reported that the conception rate at foal heat of heavy draft mares whose placentas were expelled after intramuscular administration of 50 IU of OT up to 4 hr after foaling was more than 70%. In addition, the conception rate at foal heat of mares that received OT was higher than that of non-OT treated mares among the mares that expelled the placenta 1-4 hr after foaling [5]. However, whether this is a more effective method of treatment to not only induce expulsion of the placenta, but also to improve subsequent reproductive performance after foaling, has not yet been clarified. Prostaglandin (PG) F 2α might play a role in the contraction stimulus of the myometrium during delivery. The action and release of OT and PG during delivery are mutually and closely related [1,15].The aim of present study was to establish an e...

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Biotin-streptavidin amplified enzymeimmunoassay for 13, 14-dihydro-15-keto-PGF2α

Cited by 14 publications

References 9 publications

Comparison of endometrial prostanoid profiles in three infertile subgroups: the missing part of receptivity?

Comparison of endometrial prostanoid profiles in three infertile subgroups: the missing part of receptivity?

Evidence for Uterine Horn Differences in Local Concentrations of Oxytocin, Oxytocin Receptor and Prostaglandin F_2α during the Estrous Cycle and Early Pregnancy in Cows

Effective Oxytocin Treatment on Placental Expulsion after Foaling in Heavy Draft Mares

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Biotin-streptavidin amplified enzymeimmunoassay for 13, 14-dihydro-15-keto-PGF2α

Cited by 14 publications

References 9 publications

Comparison of endometrial prostanoid profiles in three infertile subgroups: the missing part of receptivity?

Comparison of endometrial prostanoid profiles in three infertile subgroups: the missing part of receptivity?

Evidence for Uterine Horn Differences in Local Concentrations of Oxytocin, Oxytocin Receptor and Prostaglandin F2α during the Estrous Cycle and Early Pregnancy in Cows

Effective Oxytocin Treatment on Placental Expulsion after Foaling in Heavy Draft Mares

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Evidence for Uterine Horn Differences in Local Concentrations of Oxytocin, Oxytocin Receptor and Prostaglandin F_2α during the Estrous Cycle and Early Pregnancy in Cows