Biotite-Calx Based Traditional Indian Medicine Sahastraputi-Abhrak-Bhasma Prophylactically Mitigates Allergic Airway Inflammation in a Mouse Model of Asthma by Amending Cytokine Responses

Abstract

Purpose Asthma is a heterogeneous airway inflammatory disease with limited therapeutic options. Traditional medicine is extensively used for treating various ailments including asthma. Sahastraputi-Abhrak-Bhasma (SPAB) is a biotite-calx based Indian medicine. Methods We have tested for the anti-inflammatory and anti-asthmatic properties of SPAB, using a mouse model of ovalbumin-induced allergic asthma in-vivo and cell-based assays in-vitro. Histological analysis, qPCR and ELISA were performed to assess the pathology. SEM, EDX and XRD-analysis were performed to characterize the SPAB particles. Results SEM, EDX and XRD-analysis identified the presence of SPAB particle of 100 nm–~1µm diameter and contains annite-1M, aluminium silicate, kyanite, aluminium oxide, magnesium silicate, and maghemite in the samples. Ova-challenge resulted in severe inflammatory responses, airway remodelling and increased oxidative burden in lungs. Importantly, prophylactic treatment with SPAB significantly attenuated allergen induced leukocyte infiltration specifically eosinophils, lymphocytes, macrophages and neutrophils in BALF. Ova-induced mucus hypersecretion, peri-bronchial collagen deposition, inflammatory cell infiltration and bronchial epithelial thickening were significantly abrogated upon SPAB treatment. qPCR and ELISA analysis identified that allergen induced increases in IL-5, IL-13, IL-33, IFN-γ and IL-1β cytokines mRNA in whole lungs and the levels of IL-6, IL-1β and TNF-α proteins in BALF were significantly attenuated upon oral SPAB treatment. SPAB restored allergen induced decreases in anti-oxidant markers in lungs. In-vitro, SPAB attenuated the secretion of IL-6, and TNF-α from human bronchial epithelial cells and modestly inhibited NF-kB/AP-1 pathway in HEK cells. Conclusion Taken together, our results experimentally validated the prophylactic ameliorative potential of the Indian classical medicine Sahastraputi-Abhrak-Bhasma against asthma associated airway inflammation.

Assessment of TNF‐α (‐857 C/T) gene polymorphism in oral lichen planus disease: A case–control study

“…Several studies have shown the association between TNF‐α (‐857 C/T) and autoimmune or inflammatory disorders 31 . However, the relationship between this polymorphism and OLP disease has not yet been investigated 20,21 .…”

“…Several studies have shown the association between TNF‐α (‐857 C/T) and autoimmune or inflammatory disorders. 31 However, the relationship between this polymorphism and OLP disease has not yet been investigated. 20 , 21 Limited studies with contradictory results have been done on other TNF‐α gene polymorphisms with inflammatory diseases.…”

Understanding the effects of Abhraka Bhasma on genotoxicity and its DNA repair potential in mouse model

“…Balakrishna et al. [ 8 ] showed dose-dependent increased immunomodulatory effects of Abhraka Bhasma .…”

“…It is also considered as rasayana and comprehensively used as a rejuvenator, aphrodisiac, anti-ageing and health promoter. There are evidence on hepatoprotective [ [4] , [5] , [6] ], anti-diuretic [ 7 ], anti-stress, anti-inflammatory and immunomodulatory [ 8 ], adaptogenic [ 9 ], memory-enhancing [ 10 ], anti-cancer [ 11 ], and anti-diabetic [ 3 ] role of Abhraka Bhasma .…”