Biotransformation and pharmacokinetic studies of four alkaloids from Uncaria rhynchophylla in rat plasma by ultra-performance liquid chromatography with tandem mass spectrometry

Jiang, Shuang; Yang, Xin‐Rong; Wang, Zhibin; Gan, Chunli; Jing, Huang; Sun, Jiahui; Peng, Hesong; Wei, Fanshu; Wang, Zhengyang; Yang, Chunjuan

doi:10.1016/j.jpba.2022.114858

Cited by 4 publications

(2 citation statements)

References 27 publications

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“…An appropriate plasma sample pretreatment should maximize extraction rates, minimize matrix effects, and reduce operating time [19][20][21]. Protein precipitation (PPT), solid-phase extraction (SPE) and LLE, the three most commonly used plasma sample pretreatment methods, were compared in this study [22]. The result showed that the extraction recovery rate of PPT was high, but the stability was weaker than that of LLE.…”

Section: Selection Of Extraction Methodsmentioning

confidence: 99%

Simultaneous determination and pharmacokinetics study of three triterpenoid saponins in rat plasma by ultra‐high‐performance liquid chromatography tandem mass‐spectrometry after oral administration of Astragalus Membranaceus leaf extract

Wang,

Borjigin,

Zhang

et al. 2023

J of Separation Science

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A selective and sensitive ultra‐high‐performance liquid chromatography‐tandem mass spectrometry method was developed and validated for the determination of three triterpenoid saponins isolated from Astragalus membranaceus leaf extract. In this article, a method for simultaneous determination of Huangqiyenin A, Huangqiyenin E, and Huangqiyenin K was established for the first time. The method was successfully applied to the pharmacokinetic study of Astragalus membranaceus leaf extract after oral administration. Liquid–liquid extraction was applied to plasma sample preparation. Multiple reaction monitoring mode with an electrospray ion source in positive electrospray ionization was chosen to quantify the analytes. Chromatographic separation was performed on a Waters HSS T3 column, using gradient elution with a mobile phase composed of acetonitrile and 5 mM ammonium acetate/water. The pharmacokinetic results showed that all three compounds had the characteristics of rapid absorption‐slow metabolism trend. The time of maximum plasma concentration of Huangqiyenin A is higher than Huangqiyenin E and Huangqiyenin K. And the maximum plasma concentration of Huangqiyenin A is higher as well.The pharmacokinetic results revealed the pharmacokinetic characteristics of the three analytes in rat plasma, which could provide a helpful reference for the further study of Astragalus membranaceus leaf extract.

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Section: Selection Of Extraction Methodsmentioning

confidence: 99%

Simultaneous determination and pharmacokinetics study of three triterpenoid saponins in rat plasma by ultra‐high‐performance liquid chromatography tandem mass‐spectrometry after oral administration of Astragalus Membranaceus leaf extract

Wang,

Borjigin,

Zhang

et al. 2023

J of Separation Science

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“…Rhynchophylline, a major bioactive substance found in Uncaria plants, has a long history of clinical application in the treatment of cardiovascular and cerebrovascular diseases in traditional Chinese medicine (Jiang et al, 2022). It has comprehensive biological effects on the nervous system and are expected to treat AD in multiple targets and multiple pathways.…”

Section: Rhynchophyllinementioning

confidence: 99%

Update on new trends and progress of natural active ingredients in the intervention of Alzheimer's disease, based on understanding of traditional Chinese and Western relevant theories: A review

Rang

Liu

et al. 2023

Phytotherapy Research

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Alzheimer's disease (AD) is one of the major neurological disorders causing death in the elderly worldwide. As a neurodegenerative disease that is difficult to prevent and cure, the pathogenesis of AD is complex and there is no effective cure. A variety of natural products derived from plants have been reported to have promising anti‐AD activities, including flavonoids, terpenes, phenolic acids and alkaloids, which can effectively relieve the symptoms of AD in a variety of ways. This paper mainly reviews the pharmacological activity and mechanisms of natural products against AD. Although the clinical efficacy of these plants still needs to be determined by further high‐quality studies, it may also provide a basis for future researchers to study anti‐AD in depth.

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Advanced researches of traditional uses, phytochemistry, pharmacology, and toxicology of medical Uncariae Ramulus Cum Uncis

Cao,

Wang,

Zhou

et al. 2024

Journal of Ethnopharmacology

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Biotransformation and pharmacokinetic studies of four alkaloids from Uncaria rhynchophylla in rat plasma by ultra-performance liquid chromatography with tandem mass spectrometry

Cited by 4 publications

References 27 publications

Simultaneous determination and pharmacokinetics study of three triterpenoid saponins in rat plasma by ultra‐high‐performance liquid chromatography tandem mass‐spectrometry after oral administration of Astragalus Membranaceus leaf extract

Simultaneous determination and pharmacokinetics study of three triterpenoid saponins in rat plasma by ultra‐high‐performance liquid chromatography tandem mass‐spectrometry after oral administration of Astragalus Membranaceus leaf extract

Update on new trends and progress of natural active ingredients in the intervention of Alzheimer's disease, based on understanding of traditional Chinese and Western relevant theories: A review

Advanced researches of traditional uses, phytochemistry, pharmacology, and toxicology of medical Uncariae Ramulus Cum Uncis

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