Biotransformation of amide using Bacillus sp.: isolation strategy, strain characteristics and enzyme immobilization

Abstract

Amidase hydrolases/acyltransferases are of considerable industrial interest due to potential applications in the production of useful hydroxamic acids. The test strain, capable of acetamide degradation, was isolated by an enrichment technique with acetamide as sole source of nitrogen. Based on morphology, physiological tests and biochemical tests, this isolate was identified as Bacillus sp. and on the basis of 16S Ribosomal ribonucleic acid sequence, a phylogenetic tree was drawn and was identified as Bacillus megaterium. Resting cells containing active acyltransferase enzyme were prepared and immobilized in the gel beads of sodium alginate, agar, polyacrylamide and polyvinyl alcohol-alginate. The beads were tested for acyltransferase using Iron (III) chloride reagents at 55°C and were found to be affected by substrate concentration, type of buffer, buffer pH and reaction temperature. These factors were optimized using sodium alginate immobilized beads. This study proved useful in understanding the technique of immobilization of acyltransferase enzyme, its operational stability and its importance in the synthesis of hydroxamic acid.

Biodegradation of di-n-butyl phthalate in sequencing batch reactor bioaugmented with Micrococcus sp. and the bacterial community analysis

“…Bioaugmentation has been used to promote the start-up of a reactor, to protect the existing microbial community against adverse effects and to accelerate the removal of xenobiotics as a promising strategy for wastewater treatment Abdulsalam et al 2011;Sogani et al 2012). Wang et al (2002) studied the bioaugmentation for the removal of quinoline in coke plant wastewater by inoculating a quinoline-degrading bacterium Burkholderia pickettii in an anaerobic-anoxic-oxic system and found that bioaugmentation was an efficient strategy for increasing the removal of recalcitrant compounds from industrial wastewater.…”

Immobilization of Lipase using Alginate Hydrogel Beads and Enzymatic Evaluation in Hydrolysis of p-Nitrophenol Butyrate

“…Enzyme immobilization has been studied and utilized to overcome these problems. [8][9][10][11] The enzyme immobilization is to entrap the enzymes in a semi-permeable supports, which prevents the enzyme from leaving while allowing substrates and products to pass through. 12 Immobilized enzymes typically exhibit greater stability and enzymatic activity over a broad range of pH and temperature.…”

Biodegradation of 2,2' 4,4-tetrabromodiphenyl Ether in an Aerobic Environment by a Novel Strain of <i>Bacillus sp.</i>

“…About degradation function Bacillus sp., most come from the polluted soil [11,12] , and some came from sediment [13] and water pollution [14] . Bacillus species are attractive industrial organisms not only because of their high growth rates leading to short fermentation cycle times, but also the spore-forming can resist many adverse environments.…”