Biotransformation of geraniol by photoautotrophic, photomixotrophic and heterotrophic plant cell suspensions

Carrière, Frédéric; Gil, Gtérard; Tapie, P.; Chagvardieff, P.

doi:10.1016/0031-9422(89)80188-8

Cited by 24 publications

(7 citation statements)

References 12 publications

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“…Biotransformation of geraniol has also been observed in cell suspension cultures C. roseus (Carriere et al 1989). This compound rapidly disappeared after its addition to the cultures.…”

Section: Bioconversionsmentioning

confidence: 86%

Cell and tissue cultures of Catharanthus roseus: A literature survey

Moreno

Heijden

Verpoorte

1995

Plant Cell Tiss Organ Cult

217

100

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The literature concerning the regulation and the biosynthesis of secondary metabolites in cell and tissue cultures of Catharanthus roseus is reviewed. The aim of this review is to summarise the progress achieved since the previous review of this subject from 1988 to December 1993. Several factors influencing the production of indole alkaloids are discussed. Special attention is given to large-scale cultivation methods. Some economic considerations on the production of ajmalicine are also discussed. ranth~ ro~eu~ cited in ~ C~c~ Abs~acts ~tween 1950 ~d 1993.most valuable components, the dimeric alkaloids vincristine and vinblastine, have so far only been detected in callus or organ cultures of C. roseus (Miura & Hirata 1982; Hoffmann et al. 1982;Miura et al. 1988).

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“…Biotransformation of geraniol has also been observed in cell suspension cultures C. roseus (Carriere et al 1989). This compound rapidly disappeared after its addition to the cultures.…”

Section: Bioconversionsmentioning

confidence: 86%

Cell and tissue cultures of Catharanthus roseus: A literature survey

Moreno

Heijden

Verpoorte

1995

Plant Cell Tiss Organ Cult

217

100

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“…The lack of observable accumulation thus indicates that these suspension cultures must readily degrade monoterpenes, and the efficient degradation of exogenous camphor to water-soluble metabolites was, in fact, demonstrated. Catabolism of exogenous monoterpenes has been shown in cell cultures of a variety of other species (7,15,20).…”

Section: Resultsmentioning

confidence: 99%

“…By contrast, the efficient biotransformation of exogenous monoterpenes in cell culture ( 19) implies that at least portions of monoterpene metabolic pathways may be widely present in these systems. The ability ofplant cell cultures to catabolize added monoterpenes (1,7,15,20) suggests that degradative capability may be critically important in avoiding the toxic effects of these compounds on the growth and viability of cells in culture ( 14).…”

mentioning

confidence: 99%

Metabolism of Monoterpenes in Cell Cultures of Common Sage (Salvia officinalis)

Falk¹,

Gershenzon²,

Croteau³

1990

Plant Physiol.

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Leaves of common sage (Salvia officinalis) accumulate monoterpenes in glandular trichomes at levels exceeding 15 milligrams per gram fresh weight at maturity, whereas sage cells in suspension culture did not accumulate detectable levels of monoterpenes (<0.3 nanograms per gram fresh weight) at any stage of the growth cycle, even in the presence of a polystyrene resin trap. Monoterpene biosynthesis from [U-14C]sucrose was also virtually undetectable in this cell culture system. In vitro assay of each of the enzymes required for the sequential conversion of the ubiquitous isoprenoid precursor geranyl pyrophosphate to (+)-camphor (a major monoterpene product of sage) in soluble extracts of the cells revealed the presence of activity sufficient to produce (+)-camphor at a readily detectable level (>0.3 micrograms per gram fresh weight) at the late log phase of growth. Other monoterpene synthetic enzymes were present as well. In vivo measurement of the ability to catabolize (+)-camphor in these cells indicated that degradative capability exceeded biosynthetic capacity by at least 1000-fold. Therefore, the lack of monoterpene accumulation in undifferentiated sage cultures could be attributed to a low level of biosynthetic activity (relative to the intact plant) coupled to a pronounced capacity for monoterpene catabolism.The accumulation of terpenoid natural products in plant cell cultures has been successfully demonstrated in the cases of diterpenoids and sesquiterpenoids, but rarely in the case of monoterpenes. Thus, there are reports of the production of diterpenoid substances in culture at levels exceeding those of the intact plant (42,43) and the induced accumulation of sesquiterpene phytoalexins in culture is well documented (12,13,16), whereas most accounts ofmonoterpene accumulation in cell culture systems (1, 17, 44) describe either very low levels of production or compositional patterns that differ markedly from those of the intact plant.Monoterpenes in intact plants usually accumulate in the extracellular storage spaces of specialized secretory structures, such as glandular trichomes, resin ducts, or resin cavities (35),

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“…The observed transformation rates, however, are only in the range of mg per litre culture medium (Carriere et al 1989). Up till now a whole range of reactions has been observed including, for example, esterification, oxidation, reduction, hydroxylation, and glycosylation.…”

Section: In Vivo Biotransformations With Plant Cellsmentioning

confidence: 95%

Natural products and enzymes from plant cell cultures

Stöckigt

Obitz

Falkenhagen

et al. 1995

Plant Cell Tiss Organ Cult

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Plants represent an unlimited source of natural products. Many of the recently detected phytochemicals exhibit remarkable bioactivities, ranging from anticancer activity, phosphodiesterase inhibition to cytotoxicity against HIVinfected cells. Cultivated plant cells produce at their unorganized, dedifferentiated stage secondary metabolites, but in very different amounts in so far as new compounds are concerned. In fact, more than 140 novel natural products are presently known from plant cell cultures, which also include new metabolites formed by biotransformation. The biotransformation capacity of suspended cells is described and recent high yielding transformations, like the formation of arbutin by hydroquinone-transformation with Rauwolfia cells are discussed. As an example of alkaloid production by cell suspensions, the pattern of monoterpenoid indole alkaloids of the Indian medicinal plant Rauwolfia serpentina Benth. is described and the so far 30 identified compounds are divided into eight groups which are biosynthetically closely related. Some of the key biosynthetic reactions leading to the Rauwolfia alkaloids are discussed and an overview of the enzymes involved in the formation of the alkaloid ajmaline and proteins catalyzing side reactions of the ajmaline pathway are given.

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Biotransformation of geraniol by photoautotrophic, photomixotrophic and heterotrophic plant cell suspensions

Cited by 24 publications

References 12 publications

Cell and tissue cultures of Catharanthus roseus: A literature survey

Cell and tissue cultures of Catharanthus roseus: A literature survey

Metabolism of Monoterpenes in Cell Cultures of Common Sage (Salvia officinalis)

Natural products and enzymes from plant cell cultures

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