Biotransformation of lignocellulosic biomass to xylitol: an overview

Jain, Vasundhara; Ghosh, Sanjoy

doi:10.1007/s13399-021-01904-0

Cited by 19 publications

(4 citation statements)

References 124 publications

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“…Xylitol production using wild-type as well as genetically engineered yeast strains belonging to Candida sp., Kluyveromyces marxianus , Debaryomyces hansenii and Saccharomyces cerevisiae has been reported by several studies (Table 2 ) [ 1 , 30 ]. Among them, deletion of XYL2 in yeast harbouring active XR/XDH pathway ( C. tropicalis ) and heterologous expression of XYL1 gene in yeast lacking active XR/XDH pathway ( S. cerevisiae ) were most promising strategies [ 4 , 7 ].…”

Section: Discussionmentioning

confidence: 99%

Development of engineered Candida tropicalis strain for efficient corncob-based xylitol-ethanol biorefinery

Singh,

Deeba,

Kumar

et al. 2023

Microb Cell Fact

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Background Xylitol has a wide range of applications in the pharmaceuticals, cosmetic, food and beverage industry. Microbial xylitol production reduces the risk of contamination and is considered as environment friendly and sustainable compared to the chemical method. In this study, random mutagenesis and genetic engineering approaches were employed to develop Candida tropicalis strains with reduced xylitol dehydrogenase (XDH) activity to eliminate co-substrate requirement for corn cob-based xylitol-ethanol biorefinery. Results The results suggest that when pure xylose (10% w/v) was fermented in bioreactor, the Ethyl methane sulfonate (EMS) mutated strain (C. tropicalis K2M) showed 9.2% and XYL2 heterozygous (XYL2/xyl2Δ::FRT) strain (C. tropicalis K21D) showed 16% improvement in xylitol production compared to parental strain (C. tropicalis K2). Furthermore, 1.5-fold improvement (88.62 g/L to 132 g/L) in xylitol production was achieved by C. tropicalis K21D after Response Surface Methodology (RSM) and one factor at a time (OFAT) applied for media component optimization. Finally, corncob hydrolysate was tested for xylitol production in biorefinery mode, which leads to the production of 32.6 g/L xylitol from hemicellulosic fraction, 32.0 g/L ethanol from cellulosic fraction and 13.0 g/L animal feed. Conclusions This work, for the first time, illustrates the potential of C. tropicalis K21D as a microbial cell factory for efficient production of xylitol and ethanol via an integrated biorefinery framework by utilising lignocellulosic biomass with minimum waste generation.

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Section: Discussionmentioning

confidence: 99%

Development of engineered Candida tropicalis strain for efficient corncob-based xylitol-ethanol biorefinery

Singh,

Deeba,

Kumar

et al. 2023

Microb Cell Fact

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“…The first possible chemicals from lignocellulose are C5 and C6 sugars, further convertible into a variety of platform chemicals [275], such as organic acids [311], succinic acid [312], lactic acid [313], levulinic acid [314], fumaric acid [315], maleic acid [316], 2,5-furan dicarboxylic acid [317], 3-hydroxy propionic acid, aspartic acid, glucaric acid [318], glutamic acid (itaconic acid), furfural [319][320][321][322], hydroxymethylfurfural [323], 3-hydroxybutyrolactone, glycerol, sorbitol [324], and xylitol/arabitol [325,326]. These chemicals are, in turn, basic building blocks in chemical industries.…”

Section: Platform Chemicalsmentioning

confidence: 99%

Lignocellulosic Agricultural Waste Valorization to Obtain Valuable Products: An Overview

et al. 2023

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The sustainable management of lignocellulosic agricultural waste has gained significant attention due to its potential for the production of valuable products. This paper provides an extensive overview of the valorization strategies employed to convert lignocellulosic agricultural waste into economically and environmentally valuable products. The manuscript examines the conversion routes employed for the production of valuable products from lignocellulosic agricultural waste. These include the production of biofuels, such as bioethanol and biodiesel, via biochemical and thermochemical processes. Additionally, the synthesis of platform chemicals, such as furfural, levulinic acid, and xylose, is explored, which serve as building blocks for the manufacturing of polymers, resins, and other high-value chemicals. Moreover, this overview highlights the potential of lignocellulosic agricultural waste in generating bio-based materials, including bio-based composites, bio-based plastics, and bio-based adsorbents. The utilization of lignocellulosic waste as feedstock for the production of enzymes, organic acids, and bioactive compounds is also discussed. The challenges and opportunities associated with lignocellulosic agricultural waste valorization are addressed, encompassing technological, economic, and environmental aspects. Overall, this paper provides a comprehensive overview of the valorization potential of lignocellulosic agricultural waste, highlighting its significance in transitioning towards a sustainable and circular bioeconomy. The insights presented here aim to inspire further research and development in the field of lignocellulosic waste valorization, fostering innovative approaches and promoting the utilization of this abundant resource for the production of valuable products.

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“…6,7 Xylanolytic enzymes are a group of enzymes consisting of ⊍-Larabinofuranosidase (EC 3.2.1.37), endo-1,4-⊎-xylanase (EC 3.2.1.8), ⊍-glucuronidase (EC 3.2.1.139), ⊎-1,4-xylosidase (EC 3.2.1.37) and so on. 8 Among them, endo-1,4-⊎-xylanase is the most important enzyme for the degradation of xylan, which breaks the ⊎- (1,4)glycoside bond between xyloses (Xyl) from the inside and changes the distribution of arabinose (Ara). 9 According to the Carbohydrate Activated Enzymes (CAZy) database (http://www.cazy.org/fam/ acc_GH.html), xylanases from different sources (bacteria, fungi, yeasts, plants, and insects) have been classified into nine glycoside hydrolase (GH) families: GH5 (subfamilies 4 and 25), GH7, GH8, GH10, GH11, GH30 (subfamilies 7 and 8), GH43, GH51 and GH141.…”

Section: Introductionmentioning

confidence: 99%

“…Lignocellulosic biomass (LCB) is an abundant, cheap, and promising source of renewable energy consisting mainly of polysaccharide cellulose and hemicellulose and the aromatic polymer lignin, 1 including agricultural residues, such as straw, rice straw, bagasse, and corncobs. It can be efficiently converted to ethanol, xylitol, acetic acid, glutamic acid, glucuronic acid, succinic acid, and vanillin through thermochemical and biochemical pathways 2 .…”

Section: Introductionmentioning

confidence: 99%

Studies on the degradation pattern of wheat malt endo‐1,4‐β‐xylanase on wheat‐derived arabinoxylans

Fan,

Jin,

Liu

et al. 2024

J Sci Food Agric

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BACKGROUNDWheat malt endo‐1,4‐β‐xylanase is a key enzyme for arabinoxylan degradation, but its wheat‐derived arabinoxylan degradation pattern is unclear.RESULTSWater‐extractable arabinoxylan (WEAX) of 300–750 kDa and 30–100 kDa were the two components with the highest degradation efficiency of wheat malt endo‐1,4‐β‐xylanase, followed by > 1000 kDa WEAX, but 100–300 kDa WEAX showed the lowest degradation efficiency. The main enzymatic products were the 5–30 kDa WEAX, which accounted for 57.57%, 68.15%, and 52.28% of WAXH, WAXM, and WAXL products, respectively. The enzymatic efficiency of wheat malt endo‐1,4‐β‐xylanase was relatively high, and the continuity of enzymatic efficiency was good, especially since the enzymatic reaction was the most intense in 1–3 h. WEAX of > 300 kDa was highly significant and positively correlated with viscosity. In comparison, WEAX of < 30 kDa was highly significant and negatively correlated with viscosity. As the enzymatic degradation proceeded, there were fewer and fewer macromolecular components but more and more small molecule components, and the system viscosity became smaller and smaller.CONCLUSIONIn this study, it was found that wheat malt endo‐1,4‐β‐xylanase degraded preferentially 300–750 kDa and 30–100 kDa WEAX, not in the order of substrate size in a sequential enzymatic degradation. Wheat malt endo‐1,4‐β‐xylanase was most efficient within 3 h, primarily generating < 30 kDa WEAX ultimately. The main products were highly significantly negatively correlated with the system viscosity, so that the system viscosity gradually decreased as the enzymatic hydrolysis proceeded. © 2024 Society of Chemical Industry.

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Biotransformation of lignocellulosic biomass to xylitol: an overview

Cited by 19 publications

References 124 publications

Development of engineered Candida tropicalis strain for efficient corncob-based xylitol-ethanol biorefinery

Development of engineered Candida tropicalis strain for efficient corncob-based xylitol-ethanol biorefinery

Lignocellulosic Agricultural Waste Valorization to Obtain Valuable Products: An Overview

Studies on the degradation pattern of wheat malt endo‐1,4‐β‐xylanase on wheat‐derived arabinoxylans

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