Biotreatment of azo dye containing textile industry effluent by a developed bacterial consortium immobilised on brick pieces in an indigenously designed packed bed biofilm reactor

Abstract

This study highlights the development of a lab-scale, indigenously designed; Packed-Bed Bio lm Reactors (PBBR) packed with brick pieces. The developed bio lm in the reactor was used for the decolourisation and biodegradation of the textile industry e uent. The PBBR was continuously operated for 264 days, during which 301 cycles of batch and continuous treatment were operated. In batch mode under optimised conditions, more than 99% dye decolourisation and ≥92% COD reduction were achieved in 6 h of contact time upon supplementation of e uent with 0.25 g L -1 glucose, 0.25 g L -1 urea, and 0.1 g L -1 phosphates. A decolourisation rate of 133.94 ADMI units h -1 was achieved in the process. PBBR, when operated in continuous mode, showed ≥95% and ≥92% reduction in ADMI and COD values. Subsequent aeration and passage through charcoal reactor assisted in achieving ≥96 % reduction in COD and ADMI values. An overall increase of 81% in dye-laden e uent decolourisation rate, from 62 mg L -1 h -1 to 262 mg L -1 h -1 , was observed upon increasing the ow rate from 18 mL h -1 to 210 mL h -1 . Dye biodegradation was determined by UV-Vis and FTIR spectroscopy and toxicity study. SEM analysis showed the morphology of the attached-growth bio lm. HighlightsPacked-Bed Bio lm Reactor with an indigenous consortium was developed for textile e uent treatment.The use of developed consortia and optimisation resulted in more than 92% of COD and 99% of dye removal.FTIR analysis was conducted for the detection and quanti cation of the dye metabolites. SEM analysis was used to examine the morphology of the attached-growth bio lm.The developed reactor was successfully run for 264 d with 301 cycles.

Biodecolorization of Reactive Red 120 Azo Dye by Metal-Ion Tolerant Lysinibacilluscapsici PB300 (T) Bacteria Bacteria and its Application Potential in Textile Effluents Treatment

“…When it comes to wastewater treatment and dye bioremediation, microbial consortia work better than pure isolates. Multiple microbial compounds within the consortium co-metabolize, contributing to the expected complete and rapid destruction of the target pollutants [45]. Sphingomonas paucimobilis, Rhizobium radiobacter, and B. Subtilis were the three bacterial species that revealed high decolourization of azo dye decolourization in bacterial consortium throughout a single culture system.…”

Recent Advances in Efficient Photocatalytic Degradation Approaches for Azo Dyes

“…At 40 ppm for reactive orange 2RL and 20 ppm for reactive red M-2BF, maximum decolorization was obtained. Agarwal et al [33] developed a bioflm reactor for the decolorization of dyes. Te reactor was continuously operated for 264 days, achieving a ≥96% reduction in COD and ADMI values.…”