Biotyping and genotypic diversity among oral Candida albicans strains from caries-free and caries-active healthy children

Abstract

Candida albicans oral strains collected from caries-free and caries-active healthy children ranging from 24 to 36 months old, were studied. The aim of the study was to determine proteinase and phospholipase activities produced by Candida albicans in the two groups and to determine the phenotypic diversity of these enzymes based on genetic polymorphism using the AP-PCR method. Strains identified by morphological and fermentation tests as C. albicans were grown in proteinase and phospholipase agar media at 37ºC for 7 and 4 days, respectively. After the incubation period, the enzyme activity of the proteinase and phospholipase positive strains was measured. All strains were subjected to AP-PCR, using the arbitrary primer AP-3. The enzymatic analysis showed no differences between the two groups. The AP-PCR method was effective in demonstrating intra-individual genetic polymorphism in C. albicans, showing a greater clonal diversity in caries-active versus caries-free children. Dendograms of similarity showed only intra-individual clonal lineage. The results suggest that the enzymatic profile does not depend on the genotypic characteristics of the strains.

Differential Proteinase Patterns among Candida albicans Strains Isolated from Root Canal and Lingual Dorsum: Possible Roles in Periapical Disease

“…In particular, samples isolated from root canals generated larger albumin degradation halos. Our results are in accord with those of Mardegan et al (22), who emphasized that different ecosystems have specific characteristics that limit or induce the expression of enzymes and generate different levels of secretion. Similarly, Schaller et al (23) detected reduced levels of enzyme secretion among commensal isolates of C. albicans.…”

Microbiota of Plaque Microcosm Biofilms: Effect of Three Times Daily Sucrose Pulses in Different Simulated Oral Environments

“…albicans is a persistent [Starr et al, 2002] widespread member of the oral microbiota of increased abundance and diversity in children with high caries [Hossain et al, 2003;Mardegan et al, 2006;Moalic et al, 2001]. It is highly acidogenic and in one study released calcium from hydroxyapatite at 20 times the rate of S. mutans [Nikawa et al, 2002].…”

Effect of garlic-derived allyl sulphides on morphogenesis and hydrolytic enzyme secretion inCandida albicans

“…MIC values of both DAS and DADS against 24 C. albicans strains were determined by the micro broth dilution method [4].…”