Biotyping and Genotyping (MLVA16) of Brucella abortus Isolated from Cattle in Brazil, 1977 to 2008

Minharro, Sílvia; Mol, Juliana Pinto da Silva; Dorneles, Elaine Maria Seles; Pauletti, Rebeca Barbosa; Neubauer, Heinrich; Melzer, Falk; Poester, Fernando Padilla; Dasso, Maurício Gautério; Pinheiro, Elaine S.; Filho, Paulo Martins Soares; Santos, Renato L.; Heinemann, Marcos Bryan; Lage, Andrey Pereira

doi:10.1371/journal.pone.0081152

Cited by 31 publications

(35 citation statements)

References 26 publications

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“…Analysis of the panel A loci (MLVA-8) revealed four genotypes, with genotype 36 being the most common among the strains (91%, n = 86). Four strains were found to belong to genotype 28, two strains to genotype 38, and two strains to genotype 167,which has been detected earlier in Brazil [45]. Three loci that contributed to discrimination in the set of MLVA-8 were Bruce 06 (HGDI of 0.083), Bruce 11, and Bruce 55 with an HGDI of 0.082.…”

Section: Resultsmentioning

confidence: 62%

Epidemiology of Brucellosis and Genetic Diversity of Brucella abortus in Kazakhstan

et al. 2016

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Brucellosis is a major zoonotic infection in Kazakhstan. However, there is limited data on its incidence in humans and animals, and the genetic diversity of prevalent strains is virtually unstudied. Additionally, there is no detailed overview of Kazakhstan brucellosis control and eradication programs. Here, we analyzed brucellosis epidemiological data, and assessed the effectiveness of eradication strategies employed over the past 70 years to counteract this infection. We also conducted multiple loci variable-number tandem repeat analysis (MLVA) of Brucella abortus strains found in Kazakhstan. We analyzed official data on the incidence of animal brucellosis in Kazakhstan. The records span more than 70 years of anti-brucellosis campaigns, and contain a brief description of the applied control strategies, their effectiveness, and their impact on the incidence in humans. The MLVA-16 method was used to type 94 strains of B. abortus and serial passages of B. abortus 82, a strain used in vaccines. MLVA-8 and MLVA-11 analyses clustered strains into a total of four and seven genotypes, respectively; it is the first time that four of these genotypes have been described. MLVA-16 analysis divided strains into 28 distinct genotypes having genetic similarity coefficient that varies from 60 to100% and a Hunter & Gaston diversity index of 0.871. MST analysis reconstruction revealed clustering into "Kazakhstani-Chinese (Central Asian)", "European" and "American" lines. Detection of multiple genotypes in a single outbreak confirms that poorly controlled trade of livestock plays a crucial role in the spread of infection. Notably, the MLVA-16 profile of the B. abortus 82 strain was unique and did not change during 33 serial passages. MLVA genotyping may thus be useful for epidemiological monitoring of brucellosis, and for tracking the source(s) of infection. We suggest that countrywide application of MLVA genotyping would improve the control of brucellosis in Kazakhstan.

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Section: Resultsmentioning

confidence: 62%

Epidemiology of Brucellosis and Genetic Diversity of Brucella abortus in Kazakhstan

et al. 2016

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“…The MLVA16 panel 1 profiles of both B. abortus isolates, genotypes 28 and 40 by the MLVAbank, have already been previously observed in Minas Gerais State [11]. Moreover, the comparison between MLVA16 genotypes of B. abortus biovar 3 field strain (W) and those previously described by Minharro et al [11] revealed differences restricted to hypervariable loci [panels 2A (Bruce19) and 2B (Bruce04)], suggesting a possible epidemiological link between these strains, since all were isolated in the state of Minas Gerais and were also identified as biovar 3.…”

Section: Discussionmentioning

confidence: 73%

Genetic stability of Brucella abortus isolates from an outbreak by multiple-locus variable-number tandem repeat analysis (MLVA16)

et al. 2014

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BackgroundBrucellosis caused by Brucella abortus is one of the most important zoonoses in the world. Multiple-locus variable-number tandem repeat analysis (MLVA16) has been shown be a useful tool to epidemiological traceback studies in B. abortus infection. Thus, the present study aimed (i) to evaluate the genetic diversity of B. abortus isolates from a brucellosis outbreak, and (ii) to investigate the in vivo stability of the MLVA16 markers.ResultsThree-hundred and seventy-five clinical samples, including 275 vaginal swabs and 100 milk samples, were cultured from a brucellosis outbreak in a cattle herd, which adopted RB51 vaccination and test-and-slaughter policies. Thirty-seven B. abortus isolates were obtained, eight from milk and twenty-nine from post-partum/abortion vaginal swabs, which were submitted to biotyping and genotyping by MLVA16. Twelve B. abortus isolates obtained from vaginal swabs were identified as RB51. Twenty four isolates, seven obtained from milk samples and seventeen from vaginal swabs, were identified as B. abortus biovar 3, while one isolate from vaginal swabs was identified as B. abortus biovar 1. Three distinct genotypes were observed during the brucellosis outbreak: RB observed in all isolates identified as RB51; W observed in all B. abortus biovar 3 isolates; and Z observed in the single B. abortus biovar 1 isolate. Epidemiological and molecular data show that the B. abortus biovar 1 genotype Z strain is not related to the B. abortus biovar 3 genotype W isolates, and represents a new introduction B. abortus during the outbreak.ConclusionsThe results of the present study on typing of multiple clinical B. abortus isolates from the same outbreak over a sixteen month period indicate the in vivo stability of MLVA16 markers, a low genetic diversity among B. abortus isolates and the usefulness of MLVA16 for epidemiological studies of bovine brucellosis.

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“…The mPCR validated in the present research has identified different Brucella species and was able to discriminate the vaccine strain S19, field strain and different groups of biovars found in Brazil (MINHARRO et al, 2013). Originally described AMOS PCR can not detect biovars 3, 5, 6 and 9 and does not any internal control for DNA extraction and PCR inhibition, except when working with differentiation of vaccine samples (BRICKER & HALLING, 1995).…”

Section: Reproducibilitymentioning

confidence: 67%

Validation of the multiplex PCR for identification of Brucella spp.

et al. 2016

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Biotyping and Genotyping (MLVA16) of Brucella abortus Isolated from Cattle in Brazil, 1977 to 2008

Cited by 31 publications

References 26 publications

Epidemiology of Brucellosis and Genetic Diversity of Brucella abortus in Kazakhstan

Epidemiology of Brucellosis and Genetic Diversity of Brucella abortus in Kazakhstan

Genetic stability of Brucella abortus isolates from an outbreak by multiple-locus variable-number tandem repeat analysis (MLVA16)

Validation of the multiplex PCR for identification of Brucella spp.

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