Bioviability of Beta-Tricalcium Phosphate Nanoencapsulation from Synthesis of Anadara granosa Shells on Fibroblast Cell Line BHK-21 Cell Culture

Kunarti, Sri; Budhy, Theresia Indah; Sari, Rima Parwati

doi:10.30880/ijie.2022.14.02.002

Cited by 2 publications

(3 citation statements)

References 7 publications

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“…The sample was placed in a suitable single-use plastic cuvette, then measured with a beta nanoparticle analyzer (VASCO™, Cordouan Technologies, France) times per group. 12 The optimal attenuator slit width was 6 to 8. If the attenuator gap showed the number 6, the sample needed to be dissolved, while if the attenuator showed number 8, the sample substance needed to be added again.…”

Section: Particle Size Analyzer Testmentioning

confidence: 99%

Application of Stichopus hermanni Nanoparticle Gel in the Healing of Traumatic Ulcers

et al. 2023

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Objective The aim of this research was to investigate the use of Stichopus herrmanni nanoparticle gel on the ulcer healing process by observing blood vessels, fibroblasts, and Collagen type-I (COL-1) expression on the 4 and 7th days after trauma. Materials and Methods Gold sea cucumber (Stichopus herrmanni) powder was processed by freeze-drying method, then by high-energy milling to form nanoparticle size, and then with CMC 2% to make hydrogel. Traumatic ulcers were formed by induction using a burner. Five groups of male Wistar rats, each consisting of six tails, were divided into a negative control group that was given a placebo, the positive control group was given 0.2% hyaluronic acid, and the treatment group was given gold sea cucumbers with concentrations of 0.135, 0.27, and 0.54% (SH1-SH2-SH3). Fibroblast and blood vessels were examined with hematoxylin-eosin on day 3 and 7, while COL-1 expression was examined with immunohistochemistry on day 7. The rats' mucosa was taken on the 3rd and 7th days after the traumatic ulcer was formed. Statistical Analysis The data were analyzed using a one-way analysis of variance followed by a post-hoc test with a p less than 0.05. Results Nanoparticles gel freeze-drying of Stichopus herrmanni increased blood vessels on day 3. Angiogenesis continued to occur, which resulted in increased fibroblast and COL-1 expression on day 7. Conclusions The application of Stichopus herrmanni nanoparticle gel at 0.27% effectively increased the number of blood vessels, fibroblasts, and COL-1 expression in healing traumatic ulcers.

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Section: Particle Size Analyzer Testmentioning

confidence: 99%

Application of Stichopus hermanni Nanoparticle Gel in the Healing of Traumatic Ulcers

et al. 2023

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“…Elisa reader with a wavelength of 620 nm. The result of toxicity test was carried out by calculating the percentage of fibroblast cell viability using the formula: 16 Next, the process of creating the BCP scaffold was carried out using the freeze-drying method. The freeze-drying method is a method that consists of two process sequences, which are freezing followed by drying.…”

Section: Formazan Optical Density Values Were Read With Anmentioning

confidence: 99%

“…The cytotoxicity test could be performed with in vitro methods through BHK-21 fibroblast cell culture with the MTT Assay test. 16,17 Fibroblasts perform and carry through in bone regeneration, especially in the reparative phase, where fibroblasts will synthesize collagen and form new granulation tissue which will covering wounds, Fibroblasts have the ability to grow quickly in wound tissue, are easy to culture, granosa due to its composition Odonto : Dental Journal. Volume 10.…”

Section: Introductionmentioning

confidence: 99%

Cytotoxicity and mechanical properties of biphasic calcium phosphate scaffold from Tegilarca granosa due to its composition

Prananingrum,

Caroline,

Rizal

et al. 2023

Odonto: Dental Journal

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Background: Biphasic calcium phosphate (BCP) is graft material contained HA and TCP. Tegilarca granosa shell is a natural source that may converted into BCP. This study aims to determine the composition and cytotoxicity of BCP synthesized from Tegilarca granosa shell used various hydrothermal hours and to evaluate the mechanical properties of BCP scaffold. Methods: Tegilarca granosa shell was converted into BCP using hydrothermal method at 200ËšC for 6h (Group 1); 9h (Group 2); and 12h (Group 3). The composition was determined by XRD and the cell viability were evaluated using MTT Assays. Each group was added with 20% gelatin ratio 50:50 (w/v) and freeze-dried to form scaffold. Scaffolds (Ã˜6mm x 4mm) were prepared for diametral tensile strength (DTS) test (n=6) and scaffolds (Ã˜7mm x 11mm) were used for compressive strength (CS) test (n=6). All data were analyzed using Kruskall-Wallis followed by Mann-Whitney test. Results: The composition of BCP (HA/ TCP) at Group 1, Group 2, and Group 3 were 81.80%/14,10%; 87%/6%; and 72%/21%. The cell viabilities were good for all groups. The DTS and CS test showed there was a significant difference between Group 1 and Group 3 scaffold, meanwhile there was no significant differences between Group 2 and Group 3 scaffold. Group 3 scaffold showed the highest DTS and CS, 6.921 MPa and 1,233 MPa. Conclusion: The BCP composition were depent on hydrothermal hours. Although all scaffold groups were non-toxic, but BCP scaffold synthesized from Tegilarca granosa shell using hydrothermal for 12 hours showed the highest mechanical properties.

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Bioviability of Beta-Tricalcium Phosphate Nanoencapsulation from Synthesis of Anadara granosa Shells on Fibroblast Cell Line BHK-21 Cell Culture

Cited by 2 publications

References 7 publications

Application of Stichopus hermanni Nanoparticle Gel in the Healing of Traumatic Ulcers

Application of Stichopus hermanni Nanoparticle Gel in the Healing of Traumatic Ulcers

Cytotoxicity and mechanical properties of biphasic calcium phosphate scaffold from Tegilarca granosa due to its composition

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