Biphasic effects of l-ascorbate on the tumoricidal activity of non-thermal plasma against malignant mesothelioma cells

Shi, Lei; Wang, Yue; Ito, Fumiya; Okazaki, Yasumasa; Tanaka, Hiromasa; Mizuno, Masaaki; Hori, Masaru; Richardson, Des R.; Toyokuni, Shinya

doi:10.1016/j.abb.2016.05.016

Cited by 26 publications

(20 citation statements)

References 49 publications

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“…Lipid peroxidation of membranes causes lethal damage . RONS produce organic radicals which propagate peroxidation reactions . Lipid peroxidation causes biological membranes to lose fluidity, followed by increased membrane permeability, possibly allowing the inflow of RONS .…”

Section: Introductionmentioning

confidence: 99%

Intracellular responses to reactive oxygen and nitrogen species, and lipid peroxidation in apoptotic cells cultivated in plasma-activated medium

Furuta

Kurake

Ishikawa

et al. 2017

Plasma Process Polym

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A mechanism of the cytotoxicity of plasma-activated medium (PAM) is revealed by examining the intracellular effects of reactive oxygen nitrogen species (RONS) and lipid oxidation. PAM is cell culture medium activated by irradiation using non-equilibrium atmospheric-pressure plasma using pure Ar gas in ambient air. Extracellular RONS in PAM induced the apoptotic death of HeLa cells. Temporal changes in intracellular RONS, such as ONOO − , NO, and O À 2 , were analyzed. Intracellular RONS generation in HeLa cells following incubation in PAM triggered activation of the caspase cascade pathway and lipid peroxidation of intracellular membranes to induce apoptosis. K E Y W O R D S non-equilibrium atmospheric-pressure plasma, plasma medicine plasma-activated medium (PAM), reactive oxygen and nitrogen species (RONS)

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Section: Introductionmentioning

confidence: 99%

Intracellular responses to reactive oxygen and nitrogen species, and lipid peroxidation in apoptotic cells cultivated in plasma-activated medium

Furuta

Kurake

Ishikawa

et al. 2017

Plasma Process Polym

Self Cite

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“…81). (Shi et al 2016). It is suggested that relative to NTP alone, the combination of extracellular L-ascorbate and NTP induces greater levels of cytotoxic ROS generation (a pro-oxidant role).…”

Section: Effects Of Plasma Treatmentmentioning

confidence: 99%

“…Co-localization of LC3B and b Fig. 79 Biphasic effects of L-ascorbate on the anti-tumor effect of non-thermal plasma (NTP) on mesothelioma cells (Shi et al 2016 Data are shown as mean ± SEM (n = 3). *, P \ 0.05; **, P \ 0.01; ***, P \ 0.001 vs control (Shi et al 2016).…”

Section: Effects Of Plasma Treatmentmentioning

confidence: 99%

“…The addition of L-ascorbate enhanced plasma-induced ROS in mesothelioma cells, whereas prolonged treatment with L-ascorbate decreased plasma-induced ROS [see Fig. 79, (Shi et al 2016)]. …”

Section: Effects Of Plasma Treatmentmentioning

confidence: 99%

“…79 Biphasic effects of L-ascorbate on the anti-tumor effect of non-thermal plasma (NTP) on mesothelioma cells (Shi et al 2016 Data are shown as mean ± SEM (n = 3). *, P \ 0.05; **, P \ 0.01; ***, P \ 0.001 vs control (Shi et al 2016). SM2 MM cells were treated with NTP (30 s) with or without L-ascorbate (500 lM; without preincubation) and then incubated for 4 h, following which the cells were stained with LC3B (red) or LAMP1 (green) antibodies.…”

Section: Effects Of Plasma Treatmentmentioning

confidence: 99%

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State of the art in medical applications using non-thermal atmospheric pressure plasma

Tanaka

Ishikawa

Mizuno

et al. 2017

Rev. Mod. Plasma Phys.

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106

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Plasma medical science is a novel interdisciplinary field that combines studies on plasma science and medical science, with the anticipation that understanding the scientific principles governing plasma medical science will lead to innovations in the field. Non-thermal atmospheric pressure plasma has been used for medical treatments, such as for cancer, blood coagulation, and wound healing. The interactions that occur between plasma and cells/tissues have been analyzed extensively. Direct and indirect treatment of cells with plasma has broadened the applications of non-thermal atmospheric pressure plasma in medicine. Examples of indirect treatment include plasma-assisted immune-therapy and plasma-activated medium. Controlling intracellular redox balance may be key in plasma cancer treatment. Animal studies are required to test the effectiveness and safety of these treatments for future clinical applications.Keywords Plasma medical science Á Plasma cancer therapy Á Plasma-activated medium (PAM) Á Reactive oxygen species (ROS) Abbreviations ALKAnaplastic lymphoma kinase AMD Age-related macular degeneration APF Aminophenyl Fluorescein CNV

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Reactive Oxygen Species in Plasma Medical Science (PAM and Cancer Therapy)

2019

Plasma Medical Science

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Biphasic effects of l-ascorbate on the tumoricidal activity of non-thermal plasma against malignant mesothelioma cells

Cited by 26 publications

References 49 publications

Intracellular responses to reactive oxygen and nitrogen species, and lipid peroxidation in apoptotic cells cultivated in plasma-activated medium

Intracellular responses to reactive oxygen and nitrogen species, and lipid peroxidation in apoptotic cells cultivated in plasma-activated medium

State of the art in medical applications using non-thermal atmospheric pressure plasma

Reactive Oxygen Species in Plasma Medical Science (PAM and Cancer Therapy)

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