Biphasic Hydrogels Integrating Mineralized and Anisotropic Features for Interfacial Tissue Engineering

Echave, Mari Carmen; Domingues, Rui M. A.; Gomez‐Florit, Manuel; Pedraz, José Luís; Reis, Rui L.; Orive, Gorka; Gomes, Manuela E.

doi:10.1021/acsami.9b17826

Cited by 44 publications

(48 citation statements)

References 61 publications

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“…Anisotropic hydrogel scaffolds with oriented pores or channels provide guidance cues for cells, affecting their growth direction and rate. Studies have shown that anisotropic hydrogels were advantageous for PNI and SCI treatment because they promote glial cell and axonal alignment, outgrowth, and myelination [ [182] , [183] , [184] , [185] , [186] , [187] ]. De Laporte and co-workers defined the term Anisogel, where a soft hydrogel surrounded magneto-responsive short fibers that were directionally oriented [ 157 ].…”

Section: Anisotropic 3d Hydrogel Scaffoldsmentioning

confidence: 99%

Anisotropic scaffolds for peripheral nerve and spinal cord regeneration

Xue

Shi

Kong

et al. 2021

Bioactive Materials

112

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The treatment of long-gap (>10 mm) peripheral nerve injury (PNI) and spinal cord injury (SCI) remains a continuous challenge due to limited native tissue regeneration capabilities. The current clinical strategy of using autografts for PNI suffers from a source shortage, while the pharmacological treatment for SCI presents dissatisfactory results. Tissue engineering, as an alternative, is a promising approach for regenerating peripheral nerves and spinal cords. Through providing a beneficial environment, a scaffold is the primary element in tissue engineering. In particular, scaffolds with anisotropic structures resembling the native extracellular matrix (ECM) can effectively guide neural outgrowth and reconnection. In this review, the anatomy of peripheral nerves and spinal cords, as well as current clinical treatments for PNI and SCI, is first summarized. An overview of the critical components in peripheral nerve and spinal cord tissue engineering and the current status of regeneration approaches are also discussed. Recent advances in the fabrication of anisotropic surface patterns, aligned fibrous substrates, and 3D hydrogel scaffolds, as well as their in vitro and in vivo effects are highlighted. Finally, we summarize potential mechanisms underlying the anisotropic architectures in orienting axonal and glial cell growth, along with their challenges and prospects.

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Section: Anisotropic 3d Hydrogel Scaffoldsmentioning

confidence: 99%

Anisotropic scaffolds for peripheral nerve and spinal cord regeneration

Xue

Shi

Kong

et al. 2021

Bioactive Materials

112

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“…For example, diamagnetic nanoparticles, such as CNC, and superparamagnetic particles, such as CNC decorated with MNPs, have been used to produce anisotropy within natural and synthetic hydrogels [175,176]. For example, we developed an enzymatically crosslinked gelatin-based hydrogel system, showing sections with distinct composition and architecture integrated in a single unit in order to recreate the interfaces of the musculoskeletal system, such as the tendon-to-bone transition (also known as enthesis) [177]. On one hand, CNC loading in gelatin and exposure to a uniform magnetic field (400 mT) before crosslinking resulted in anisotropic structures that allowed hASCs alignment and the deposition of ECM proteins related to tendon.…”

Section: Anisotropic Hydrogelsmentioning

confidence: 99%

Natural-Based Hydrogels for Tissue Engineering Applications

et al. 2020

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In the field of tissue engineering and regenerative medicine, hydrogels are used as biomaterials to support cell attachment and promote tissue regeneration due to their unique biomimetic characteristics. The use of natural-origin materials significantly influenced the origin and progress of the field due to their ability to mimic the native tissues’ extracellular matrix and biocompatibility. However, the majority of these natural materials failed to provide satisfactory cues to guide cell differentiation toward the formation of new tissues. In addition, the integration of technological advances, such as 3D printing, microfluidics and nanotechnology, in tissue engineering has obsoleted the first generation of natural-origin hydrogels. During the last decade, a new generation of hydrogels has emerged to meet the specific tissue necessities, to be used with state-of-the-art techniques and to capitalize the intrinsic characteristics of natural-based materials. In this review, we briefly examine important hydrogel crosslinking mechanisms. Then, the latest developments in engineering natural-based hydrogels are investigated and major applications in the field of tissue engineering and regenerative medicine are highlighted. Finally, the current limitations, future challenges and opportunities in this field are discussed to encourage realistic developments for the clinical translation of tissue engineering strategies.

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“…When the histograms did not fit to a Gaussian distribution (adjusted R-square < 0.8), we manually assigned values of FWHM = 180°, as previously described. [63,64] At least three images per condition were considered and a representative histogram for each condition was plotted.…”

Section: Nanocoating Of the Nanopatterned Surfaces With Platelet Lysatementioning

confidence: 99%

Multifunctional Surfaces for Improving Soft Tissue Integration

Vilaça

Domingues

Tiainen

et al. 2021

Adv Healthcare Materials

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Metallic implants are widely used in diverse clinical applications to aid in recovery from lesions or to replace native hard tissues. However, the lack of integration of metallic surfaces with soft tissue interfaces causes the occurrence of biomaterial-associated infections, which can trigger a complicated inflammatory response and, ultimately, implant failure. Here, a multifunctional implant surface showing nanoscale anisotropy, based on the controlled deposition of cellulose nanocrystals (CNC), and biological activity derived from platelet lysate (PL) biomolecules sequestered and presented on CNC surface, is proposed. The anisotropic radial nanopatterns are produced on polished titanium surfaces by spin-coating CNC at high speed. Furthermore, CNC surface chemistry allows to further sequester and form a coating of bioactive molecules derived from PL. The surface anisotropy provided by CNC guides fibroblasts growth and alignment up to 14 days of culture. Moreover, PL-derived biomolecules polarize macrophages toward the M2-like anti-inflammatory phenotype. These results suggest that the developed multifunctional surfaces can promote soft tissue integration to metallic implants and, at the same time, prevent bacterial invasion, tissue inflammation, and failure of biomedical metallic implants.

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Biphasic Hydrogels Integrating Mineralized and Anisotropic Features for Interfacial Tissue Engineering

Cited by 44 publications

References 61 publications

Anisotropic scaffolds for peripheral nerve and spinal cord regeneration

Anisotropic scaffolds for peripheral nerve and spinal cord regeneration

Natural-Based Hydrogels for Tissue Engineering Applications

Multifunctional Surfaces for Improving Soft Tissue Integration

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