Biphasic organo-bioceramic fibrous composite as a biomimetic extracellular matrix for bone tissue regeneration

Mishra, Manoj K.

doi:10.2741/e795

Cited by 3 publications

(5 citation statements)

References 55 publications

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“…A HA modified PCL/HA composite had better biocompatibility for hMSCs cells with higher proliferation and osteogenic potential, compared to neat PCL. Whereby the efficiency of attachment between hMSCs and the PCL/HA scaffold was improved with a higher HA content of 5% to 10% and in a HA concentrationdependent manner (Kumar et al, 2017). This means that in addition to the different components of modified ECM to affect the cell behaviors in bone regeneration, different ECM contents also play different roles.…”

Section: Ecm-modified Biomaterials Scaffoldmentioning

confidence: 99%

The Bone Extracellular Matrix in Bone Formation and Regeneration

et al. 2020

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Bone regeneration repairs bone tissue lost due to trauma, fractures, and tumors, or absent due to congenital disorders. The extracellular matrix (ECM) is an intricate dynamic bio-environment with precisely regulated mechanical and biochemical properties. In bone, ECMs are involved in regulating cell adhesion, proliferation, and responses to growth factors, differentiation, and ultimately, the functional characteristics of the mature bone. Bone ECM can induce the production of new bone by osteoblast-lineage cells, such as MSCs, osteoblasts, and osteocytes and the absorption of bone by osteoclasts. With the rapid development of bone regenerative medicine, the osteoinductive, osteoconductive, and osteogenic potential of ECM-based scaffolds has attracted increasing attention. ECM-based scaffolds for bone tissue engineering can be divided into two types, that is, ECM-modified biomaterial scaffold and decellularized ECM scaffold. Tissue engineering strategies that utilize the functional ECM are superior at guiding the formation of specific tissues at the implantation site. In this review, we provide an overview of the function of various types of bone ECMs in bone tissue and their regulation roles in the behaviors of osteoblast-lineage cells and osteoclasts. We also summarize the application of bone ECM in bone repair and regeneration. A better understanding of the role of bone ECM in guiding cellular behavior and tissue function is essential for its future applications in bone repair and regenerative medicine.

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Section: Ecm-modified Biomaterials Scaffoldmentioning

confidence: 99%

The Bone Extracellular Matrix in Bone Formation and Regeneration

et al. 2020

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“…A 75 cm 2 adhesive flask containing 15 mL of prepared cell culturing medium was used to culture the osteoblast-like MG63 cells at 37 • C in a humidified atmosphere containing 5% CO 2 in the air. The cell culturing medium was changed every second day [40,80,81]. 4.8.2.…”

Section: Cell Culturing Methods For the Osteoblast-like Cell Linementioning

confidence: 99%

“…The 3D-printed biomaterial scaffolds were dehydrated by sequentially immersing in 50, 60, 70, 90, and 100% v/v ethanol series for 15 min each and dried in air. Finally, the samples were analyzed using SEM after being sputter coated with gold [39][40][41]83].…”

Section: Cell Viability Assay Assessment For the 3d-printed Biomateri...mentioning

confidence: 99%

“…PBS was used to wash these samples twice, and then, they were immersed in methylene blue solution for 5 min. The samples were finally washed with PBS; then, cell adhesion and proliferation were evaluated using optical microscopy [40,81]. The 3D-printed biomaterial scaffolds were sterilized by exposing them to UV light for 24 h. The scaffolds were immersed in a BMP-7 solution (5 µg/mL) at 37 • C for the BMP-7 to attach to the surface of the printed scaffold, since PEI, which is part of the biomaterial scaffold, has a strong interaction with proteins.…”

Section: Osteoblast-like Cell Staining Using Methylene Bluementioning

confidence: 99%

“…Furthermore, osteoblast-like cells were introduced into the 3D biomaterial scaffold in order to generate ex vivo tissue constructs to be used for biocompatibility and cell response (cell adhesion, cell proliferation, and cell viability) investigations [36][37][38][39][40]. Hence, osteoblastlike MG63 cells from human osteosarcoma were used in this study since they are reported to be anchorage-dependent.…”

Section: Introductionmentioning

confidence: 99%

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A 3D-Printed Biomaterial Scaffold Reinforced with Inorganic Fillers for Bone Tissue Engineering: In Vitro Assessment and In Vivo Animal Studies

Sithole

Kumar

Toit

et al. 2023

IJMS

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This research aimed to substantiate the potential practicality of utilizing a matrix-like platform, a novel 3D-printed biomaterial scaffold, to enhance and guide host cells’ growth for bone tissue regeneration. The 3D biomaterial scaffold was successfully printed using a 3D Bioplotter® (EnvisionTEC, GmBH) and characterized. Osteoblast-like MG63 cells were utilized to culture the novel printed scaffold over a period of 1, 3, and 7 days. Cell adhesion and surface morphology were examined using scanning electron microscopy (SEM) and optical microscopy, while cell viability was determined using MTS assay and cell proliferation was evaluated using a Leica microsystem (Leica MZ10 F). The 3D-printed biomaterial scaffold exhibited essential biomineral trace elements that are significant for biological bone (e.g., Ca-P) and were confirmed through energy-dispersive X-ray (EDX) analysis. The microscopy analyses revealed that the osteoblast-like MG63 cells were attached to the printed scaffold surface. The viability of cultured cells on the control and printed scaffold increased over time (p < 0.05); however, on respective days (1, 3, and 7 days), the viability of cultured cells between the two groups was not significantly different (p > 0.05). The protein (human BMP-7, also known as growth factor) was successfully attached to the surface of the 3D-printed biomaterial scaffold as an initiator of osteogenesis in the site of the induced bone defect. An in vivo study was conducted to substantiate if the novel printed scaffold properties were engineered adequately to mimic the bone regeneration cascade using an induced rabbit critical-sized nasal bone defect. The novel printed scaffold provided a potential pro-regenerative platform, rich in mechanical, topographical, and biological cues to guide and activate host cells toward functional regeneration. The histological studies revealed that there was progress in new bone formation, especially at week 8 of the study, in all induced bone defects. In conclusion, the protein (human BMP-7)-embedded scaffolds showed higher regenerative bone formation potential (week 8 complete) compared to the scaffolds without protein (e.g., growth factor; BMP-7) and the control (empty defect). At 8 weeks postimplantation, protein (BMP-7) significantly promoted osteogenesis as compared to other groups. The scaffold underwent gradual degradation and replacement by new bones at 8 weeks in most defects.

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Fiber length and concentration: Synergistic effect on mechanical and cellular response in wet‐laid poly(lactic acid) fibrous scaffolds

et al. 2018

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In the area of biomaterials, fibers not only offer increased mechanical response, but also serve as an extracellular matrix mimicking morphology to direct cellular attachment and proliferation. While biologically similar in morphology, soft, and flexible hydrogel materials have low mechanical properties. For applications in tissue engineering, the lack of directional cues and attachment regions within the biogels is undesired as cells require a guide for adequate attachment and organized proliferation. In this work, we have investigated the role of poly(lactic acid) (PLA) fiber length and concentration as a reinforcement phase in a gelatin hydrogel matrix and the resultant mechanical and cellular responses. With increasing fiber length and concentration, the ultimate tensile strength, modulus, and toughness increased for the samples. Similarly, for shorter fiber lengths, the loss and storage modulus increased with fiber concentration. After seeding human mesenchymal stem cells (hMSCs) onto the neat fibrous scaffolds it was found that the fabrication process imparted no cytotoxicity. Furthermore, it was the concentrations and lengths of fiber both caused discernable differences in cell viability at the extreme values. Fibers of all lengths, when in a 4.0 wt % concentration, had a decrease in cell viability after 10 days while the 12.7 mm fibers showed a similar response at 2.0 wt %, but all stayed about 90% viability. With increased incubation time, hMSCs became elongated with increased proliferation. These results indicate that the wet-lay process is a rapid and scalable method by which fibrous 3D-scaffolds can be produced to reinforce hydrogel matrices. © 2018 Wiley Periodicals, Inc. J Biomed Mater Res Part B: Appl Biomater, 2018.

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Biphasic organo-bioceramic fibrous composite as a biomimetic extracellular matrix for bone tissue regeneration

Cited by 3 publications

References 55 publications

The Bone Extracellular Matrix in Bone Formation and Regeneration

The Bone Extracellular Matrix in Bone Formation and Regeneration

A 3D-Printed Biomaterial Scaffold Reinforced with Inorganic Fillers for Bone Tissue Engineering: In Vitro Assessment and In Vivo Animal Studies

Fiber length and concentration: Synergistic effect on mechanical and cellular response in wet‐laid poly(lactic acid) fibrous scaffolds

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