Birth and clinical pregnancy from fresh and frozen oocytes fertilized with cryopreserved testicular spermatozoa

Zeke, József; Konc, János; Kanyó, Katalin; Kriston, Rita; Cseh, Sándor

doi:10.3109/19396368.2012.656797

Cited by 6 publications

(5 citation statements)

References 11 publications

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“…The results indicated that no additional DNA damage was produced during cryopreservation using Cryopiece. DNA fragmentation could lead to embryo development stop and abortion (20,21). In our previous work, it had been proved that cryopreservation of sperm using micro-straw or ministraw would not impair sperm DNA integrity (7).…”

Section: Discussionmentioning

confidence: 99%

Cryopiece, a novel carrier with faster cooling rate, high recovery rate and retrieval rate, for individual sperm cryopreservation

Zhu¹,

Zhai²,

Hu³

et al. 2021

Transl Androl Urol

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Background: Cryopreservation of extremely few spermatozoa is still a major challenge for male fertility preservation. This study aims to evaluate the cooling rate, recovery rate, and retrieval rate, along with other parameters of spermatozoa that cryopreserved using Cryopiece, a novel carrier, for individual sperm cryopreservation.Methods: Semen samples from 60 fertile donors were collected, and each semen sample was screened for motile sperm and mixed with cryoprotective agent (CPA), and then frozen using Cryopiece, micro-straw, and mini-straws. The cooling rate, retrieval rate, and recovery rate, morphology, DNA fragmentation index (DFI) and mitochondrial membrane potential (MMP), were compared among the un-frozen sperm and the sperm cryopreserved using these carriers.Results: Cryopiece possessed the fastest cooling rate. After freeze-thaw, the average retrieval rate of sperm cryopreserved using Cryopiece was 96.25%, and the average recovery rate was 64.40%, which were higher than that of sperm cryopreserved using the other two carriers (71.42% and 54.30% for micro-straw, and 63.54% and 58.04% for mini-straw, respectively). There was no significant impact on DFI after sperm cryopreservation, and no significant difference in morphology between sperm cryopreserved using these carriers was observed. Though MMP of sperm changed significantly after cryopreservation, micro-straw maintained sperm MMP better than Cryopiece and mini-straw did, while no significant difference was observed in MMP between sperm cryopreserved using Cryopiece and mini-straw.Conclusions: Cryopiece produced satisfying retrieval and recovery rates in sperm cryopreservation and should be an ideal carrier for cryopreservation of small number of sperm.

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Section: Discussionmentioning

confidence: 99%

Cryopiece, a novel carrier with faster cooling rate, high recovery rate and retrieval rate, for individual sperm cryopreservation

Zhu¹,

Zhai²,

Hu³

et al. 2021

Transl Androl Urol

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“…The stimulation protocols, oocyte recovery, and ET techniques were used as previously described [315]. Briefly, all patients were treated with controlled ovarian stimulation by a gonadotropin-releasing hormone antagonist protocol using a starting dose of 225 IU of recombinant follicle-stimulating hormone (rFSH) (Gonal F; Merck Serono, Darmstadt, Germany).…”

Section: Methodsmentioning

confidence: 99%

Thinning and drilling laser-assisted hatching in thawed embryo transfer: A randomized controlled trial

Lê

Nguyen

et al. 2018

Clin Exp Reprod Med

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ObjectiveIn frozen and thawed embryos, the zona pellucida (ZP) can be damaged due to hardening. Laser-assisted hatching (LAH) of embryos can increase the pregnancy rate. This study compared thinning and drilling of the ZP before frozen embryo transfer (FET).MethodsPatients were randomly allocated into two groups for LAH using thinning or drilling on day 2 after thawing. Twenty-five percent of the ZP circumference and 50% of the ZP thickness was removed in the thinning group, and a hole 40 µm in diameter was made in the drilling group.ResultsA total of 171 in vitro fertilization/intracytoplasmic sperm injection FET cycles, including 85 cycles with drilling LAH and 86 cycles with thinning LAH, were carried out. The thinning group had a similar β-human chorionic gonadotropin-positive rate (38.4% vs. 29.4%), implantation rate (16.5% vs. 14.4%), clinical pregnancy rate (36.0% vs. 25.9%), miscarriage rate (5.8% vs. 2.4%), ongoing pregnancy rate (30.2% vs. 23.5%), and multiple pregnancy rate (7.0% vs. 10.6%) to the drilling LAH group. There were no significant differences in pregnancy outcomes between subgroups defined based on age (older or younger than 35 years) or ZP thickness (greater or less than 17 µm) according to the LAH method.ConclusionThe present study demonstrated that partial ZP thinning or drilling resulted in similar outcomes in implantation and pregnancy rates using thawed embryos, irrespective of women's age or ZP thickness.

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“…Indications for IVF-ET were: male factor (40%), tubal factor (34%), endometriosis (15%) and unexplained infertility (11%). The reader is referred to our previous publications for stimulation protocols, oocyte recovery and ET techniques (Kanyo & Konc, 2003;Konc et al, 2005Konc et al, , 2010Zeke et al, 2012).…”

Section: Patient Selection Controlled Ovarian Stimulation Oocyte Rementioning

confidence: 99%

“…Pregnancy was defined as a spontaneous rise in a ␤-hCG concentration at least 10 days post-transfer. Clinical pregnancy implied the presence of an intrauterine gestational sac and fetal heart beat on an ultrasound performed at 7 weeks of gestation (Zeke et al, 2012). In case of spontaneous abortion, the patient lost her biochemical or clinical pregnancy.…”

Section: Endometrial Preparation and Transfer Of Frozen-thawed Embryosmentioning

confidence: 99%

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The impact of laser-assisted hatching on the outcome of frozen human embryo transfer cycles

Kanyó

Zeke

Kriston

et al. 2016

Zygote

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SummaryBiochemical modifications of zona pellucida (ZP) result in zona hardening. Zona hardening (ZH) is induced by several factors such as advancing maternal age, in vitro culture conditions and cryopreservation and adversely effects implantation. The objective of the clinical study was to determine whether or not laser-assisted hatching (LAH) applied on day 3 frozen embryos improves the outcome of frozen embryo transfer (FET) cycles in patients with recurrent implantation failure and/or advanced female age. In total, 413 patients of different ages with recurrent implantation failure (maximum three cycles) were involved into the study. Patients were allocated randomly into LAH and control groups. On the day of FET, after thawing and just before FET, the ZP was thinned using a laser system. In the control group no treatment was applied on frozen embryo before transfer. The main outcome measures were clinical pregnancy rate. Overall, the results indicate a tendency that LAH increased (P = 0.08) clinical pregnancy. However, for patients older than 37 years, LAH increased pregnancy rates significantly (P = 0.03). In the LAH and control groups, the age of patients and the number of transferred embryos influenced pregnancy rates (P = 0.01). For patients older than 37 years, no effect of number of transferred embryos was detected (P = 0.14). The incidence of multiple pregnancies also increased in the LAH group (P = 0.01). In conclusion, in older woman, to overcome the negative effect of zona hardening, LAH could be performed on frozen embryos as a routine strategy before FET in frozen cycles in order to increase the possibility of pregnancy formation.

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Birth and clinical pregnancy from fresh and frozen oocytes fertilized with cryopreserved testicular spermatozoa

Cited by 6 publications

References 11 publications

Cryopiece, a novel carrier with faster cooling rate, high recovery rate and retrieval rate, for individual sperm cryopreservation

Cryopiece, a novel carrier with faster cooling rate, high recovery rate and retrieval rate, for individual sperm cryopreservation

Thinning and drilling laser-assisted hatching in thawed embryo transfer: A randomized controlled trial

The impact of laser-assisted hatching on the outcome of frozen human embryo transfer cycles

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