Bisphenol A Exposure During Early Development Induces Sex-Specific Changes in Adult Zebrafish Social Interactions

Abstract

Developmental bisphenol A (BPA) exposure is associated with adverse behavioral effects, although underlying modes of action remain unclear. Because BPA is a suspected xenoestrogen, the objective was to identify sex-based changes in adult zebrafish social behavior developmentally exposed to BPA (0.0, 0.1 or 1 μM) or one of two control compounds (0.1μM 17β-estradiol [E2], and 0.1 μM GSK4716, a synthetic estrogen-related receptor γ ligand). A test chamber was divided lengthwise so each arena held one fish unable to detect the presence of the other fish. A mirror was inserted at one end of each arena; baseline activity levels were determined without mirror. Arenas were divided into 3, computer-generated zones to represent different distances from mirror image. Circadian rhythm patterns were evaluated at 1–3 (= AM) and 5–8 (= PM) hr postprandial. Adult zebrafish were placed into arenas and monitored by digital camera for 5 min. Total distance traveled, % time spent at mirror image, and number of attacks on mirror image were quantified. E2, GSK4716, and all BPA treatments dampened male activity and altered male circadian activity patterns; there was no marked effect on female activity. BPA induced non-monotonic effects (response curve changes direction within range of concentrations examined) on male % time at mirror only in AM. All treatments produced increased % time at the mirror during PM. Male attacks on the mirror were reduced by BPA exposure only during AM. There were sex-specific effects of developmental BPA on social interactions and time-of-day of observation affected results.

Chronic bisphenol A exposure alters behaviors of zebrafish (Danio rerio)

“…The less obvious preference may associate with down-regulated of clock genes expression via BPA or E2 exposure in the pituitary, brain, muscle, and skin in fish (Rhee et al, 2014). BPA or E2 exposure may affect circulating pineal 5-hydroxytryptaming (5-HT) levels (Ho et al, 1985;Weber et al, 2015). As seen from Fig.…”

High Levels of Bisphenol A and Bisphenol S in Brazilian Thermal Paper Receipts and Estimation of Daily Exposure

“…Due to the adverse health effects of BPA in excessive doses noted in animals (Tsai 2006, Willhite et al 2008Rochester 2013;Robledo et al 2013;Kasper-Sonnenberg et al 2012, Yoon et al 2014Weber et al 2015) and the potential for certain human subpopulations to be exposed to high concentrations of this chemical, its use is now regulated in some countries. However, BPA is being replaced by a new analogue, "bisphenol S" (BPS, 4,4 -sulfonyldiphenol) (Figure 1) (Liao et al 2012a;.…”

Polluted Pathways: Mechanisms of Metabolic Disruption by Endocrine Disrupting Chemicals

“…For example, TF was shown to deleteriously alter normal circadian feeding patterns in mice [90•]. Additionally, population studies have associated higher urinary BPA levels with shorter sleep duration [116]; a finding supported by studies in male zebrafish demonstrating BPA-induced alterations in circadian activity [117]. Exposure to estradiol, tamoxifen, BPA, and 4-tert-octylphenol in mangrove killifish also altered expression of circadian clock genes [118•].…”