Bisphenol A Exposure Impairs Epididymal Development during the Peripubertal Period of Rats: Inflammatory Profile and Tissue Changes

Ogo, Fernanda Mithie; Siervo, Gláucia Elóisa Munhoz de Lion; Staurengo‐Ferrari, Larissa; Mendes, Leonardo de Oliveira; Luchetta, Nicla Renata; Vieira, Henrique Rodrigues; Fattori, Victor; Verri, Waldiceu A.; Scarano, Wellerson Rodrigo; Fernandes, Glaura Scantamburlo Alves

doi:10.1111/bcpt.12894

Cited by 19 publications

(21 citation statements)

References 52 publications

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“…A previous study by our group showed that the increase in neutrophils and macrophages after bisphenol A (BPA) exposure, a toxicant and environmental contaminant, was associated with tissue changes during epididymal development in peripubertal rats. 1 Similarly, our study showed alterations in the epididymal epithelium after both malathion treatments, and the abnormalities found may be related to the inflammatory response, which leads to tissue damage. Although the lower dose of malathion did not show an increase in inflammatory cells, we observed abnormalities in the tissue of 10 mg kg À1 malathion-treated animals.…”

Section: Discussionsupporting

confidence: 72%

Impairment of postnatal epididymal development and immune microenvironment following administration of low doses of malathion during juvenile and peripubertal periods of rats

et al. 2020

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Malathion is an organophosphate pesticide widely used for agricultural crops and for vector control of Aedes aegypti. Humans are exposed to this environmental contaminant by ingesting contaminated food. The juvenile and peripubertal periods are critical for the postnatal development of the epididymis and are when animals are most vulnerable to toxic agents. Since juveniles and adolescents are developing under exposure to the insecticide malathion, the aim of the present study was to evaluate the effects of exposure to low doses of malathion on postnatal epididymal development in rats. Male Wistar rats were exposed to malathion daily via gavage at doses of 10 mg kg−1 (M10 group) or 50 mg kg−1 (M50 group) for 40 days (postnatal days (PNDs) 25–65). The control group received the vehicle (0.9% saline) under the same conditions. On PND 40, the epididymides were removed, weighed and used for histological analysis and determination of the inflammatory profile and sperm count. Sperm from the vas deferens were subjected to sperm motility analysis. The M50 group showed tissue remodelling in the caput and cauda epididymides and increased neutrophil and macrophage migration in the caput epididymis. The M10 group showed decreased motile spermatozoa and IL-6 levels in the caput epididymis. Both doses decreased the IL-1β level and altered the morphology of the same region. These results show that malathion exposure may impair postnatal epididymal development. Furthermore, alterations of the immune system in the epididymal environment are presented as new findings regarding the action of malathion on the epididymis.

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Section: Discussionsupporting

confidence: 72%

Impairment of postnatal epididymal development and immune microenvironment following administration of low doses of malathion during juvenile and peripubertal periods of rats

et al. 2020

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“…Izumi et al reported that the adverse effects of BPA on sperm autogenesis in the Sertoli cells of rat testis were possibly regulated via increasing levels of inflammatory factors, such as IL-1β and IL-6 [ 30 ]. Ogo et al further showed that exposure to 200 μg/mL of BPA from PND36 to PND66 increased the expression of IL-6 in the epididymis of male Wistar rats [ 31 ]. Our current results implied that the adverse effects of perinatal BPA exposure on spermatogenesis might be related to the activation of an inflammatory response in the testis of male offspring.…”

Section: Discussionmentioning

confidence: 99%

Decreased Capacity for Sperm Production Induced by Perinatal Bisphenol A Exposure Is Associated with an Increased Inflammatory Response in the Offspring of C57BL/6 Male Mice

Yuan

Lin

et al. 2018

IJERPH

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Many previous studies have indicated the adverse effects of bisphenol A (BPA) on sperm production and quality; however, the mechanisms underlying BPA male reproductive toxicity have yet to be elucidated. The main purpose of this study was to investigate the effect of perinatal exposure to BPA on the spermatogenic capacity of male offspring, and to explore the possible influence of inflammatory responses in BPA reproductive toxicity. Twenty-one pregnant C57BL/6mice were randomly divided into three groups: a control group, a group receiving 0.2 μg/mL (LBPA), and a group receiving 2 μg/mL of BPA (HBPA), all via drinking water from gestational day 6 to the end of lactation. After weaning, one male mouse was randomly selected from each group (n = 7/group); these three mice were fed a normal diet and drinking water for 1 month. Levels of serum testosterone (T) and tumor necrosis factor (TNF)-α were then measured in all mice. Sperm count and the proportion of sperm malformation were also determined. The levels of Toll-like receptor 4 (TLR4), nuclear factor (NF)-κB, and aryl hydrocarbon receptor (AhR) protein expression in the testis tissue were determined. Analysis showed that the proportion of sperm malformation increased in the LBPA and HBPA groups (p < 0.05). Sperm count significantly decreased only in the HBPA group (p < 0.05), while the levels of serum TNF-α increased in the LBPA and HBPA groups (p < 0.05). Levels of serum T decreased significantly in the HBPA group, compared with controls (p < 0.05). Levels of TLR4 and NF-κB protein expression in the testis were significantly higher in the LBPA and HBPA groups (p < 0.05 or p < 0.01), while AhR protein expression was higher and seminiferous tubules in the testis showed more damage in the HBPA group compared to controls (p < 0.05 and p < 0.01, respectively). Our results showed that perinatal exposure to low or high doses of BPA decreased the capacity for spermatogenesis in male offspring, which may be associated with an inflammatory response activated by the TLR4/ NF-κB and AhR signaling pathways in the testis.

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“…Thus, recent works deepened the previous studies, with a particular emphasis on mononuclear phagocytes and lymphocytes present at steady state. Unfortunately, only a few studies described the polymorphonuclear cells in the steady state rodent epididymis, despite their crucial role in the early steps of the pathogen recognition that initiate the immune response (Fritz & Pabst, ; Gaytan et al ., ; Majeed, ,; Hess, ; Serre & Robaire, ; Mendes et al ., ; Ogo et al ., ).…”

Section: Immune Cellsmentioning

confidence: 97%

Of vessels and cells: the spatial organization of the epididymal immune system

et al. 2019

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Background: One third of infertility cases in couples worldwide has an exclusive male origin and immune disorders, essentially due to repetitive infections, are emerging an cause of male infertility. As the place of sperm maturation, epididymis must be preserved from excessive immune responses that may arise following infections of the male genital tract. At the same time, epididymis must set and maintain a tolerogenic environment in order not to destroy sperm cells that enter the tissue at puberty, long after the immune system has been taught to recognize self pathogens. The immune cells that populate the epididymis have raised growing interest over the last thirty years but they may be not sufficient to understand the immune balance existing in this organ, between immune response to pathogens and tolerance to spermatozoa. Indeed, immune cells are the most motile cells in the organism and need blood and lymphatic vessels to traffic between lymphoid organs and sites of infection to induce efficient responses. Objectives: To review the literature on the blood and lymphatic vessels, and on the immune cells present at steady state in the rodent epididymis (rat and mouse). Materials and methods: PubMed database was searched for studies reporting on the spatial organization of the rodent epididymal vasculature and immune cell types at steady state. This search was combined with recent findings from our team. Results: At steady state, the rodent epididymis presents with dense blood and lymphatic networks, and a large panel of immune cells distributed across the interstitum and epithelium along the organ. Conclusions: The immune system of the rodent epididymis is highly organized. Exploring its functions, especially in an infectious context, is the essential coming step before any transposition to human.

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Bisphenol A Exposure Impairs Epididymal Development during the Peripubertal Period of Rats: Inflammatory Profile and Tissue Changes

Cited by 19 publications

References 52 publications

Impairment of postnatal epididymal development and immune microenvironment following administration of low doses of malathion during juvenile and peripubertal periods of rats

Impairment of postnatal epididymal development and immune microenvironment following administration of low doses of malathion during juvenile and peripubertal periods of rats

Decreased Capacity for Sperm Production Induced by Perinatal Bisphenol A Exposure Is Associated with an Increased Inflammatory Response in the Offspring of C57BL/6 Male Mice

Of vessels and cells: the spatial organization of the epididymal immune system

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